![Figure 1. CDK2/CycA kinase activity was measured in a radioisotopic filter binding assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20,000, Substrate: Histone H1, 1 µg /50 µl, recombinant CDK2/CycA: 50 ng/50 µl.](http://www.bioprodhub.com/system/product_images/ak_products/1/sub_1/1851_7521_ka_jp_050928.jpg)
Figure 1. CDK2/CycA kinase activity was measured in a radioisotopic filter binding assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20,000, Substrate: Histone H1, 1 µg /50 µl, recombinant CDK2/CycA: 50 ng/50 µl.
![Figure 3. Dose dependence curve of CDK2/CycA kinase activity: DELFIA® data generated using Phospho-Rb (Ser807/811) Antibody #9308 to detect phosphorylation of substrate peptide (#1144) by CDK2/CycA kinase. In a 50 µl reaction, increasing amounts of CDK2/CycA and 1.5 µM substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)](http://www.bioprodhub.com/system/product_images/ak_products/1/sub_1/1852_7522_de_enz_jp_050928.jpg)
Figure 3. Dose dependence curve of CDK2/CycA kinase activity: DELFIA® data generated using Phospho-Rb (Ser807/811) Antibody #9308 to detect phosphorylation of substrate peptide (#1144) by CDK2/CycA kinase. In a 50 µl reaction, increasing amounts of CDK2/CycA and 1.5 µM substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)
![Figure 5. Staurosporine inhibition of CDK2/CycA kinase activity: DELFIA® data generated using Phospho-(Ser807/811) Antibody #9308 to detect phosphorylation of substrate peptide (#1144) by CDK2/CycA kinase. In a 50 µl reaction, 50 ng CDK2/CycA, 1.5 µM substrate peptide, 20 µM ATP and increasing amounts of staurosporine were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)](http://www.bioprodhub.com/system/product_images/ak_products/1/sub_1/1853_7522_de_inhib_jp_050928.jpg)
Figure 5. Staurosporine inhibition of CDK2/CycA kinase activity: DELFIA® data generated using Phospho-(Ser807/811) Antibody #9308 to detect phosphorylation of substrate peptide (#1144) by CDK2/CycA kinase. In a 50 µl reaction, 50 ng CDK2/CycA, 1.5 µM substrate peptide, 20 µM ATP and increasing amounts of staurosporine were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)
![Figure 4. Peptide concentration dependence of CDK2/CycA kinase activity: DELFIA® data generated using Phospho-Rb (Ser807/811) Antibody #9308 to detect phosphorylation of substrate peptide (#1144) by CDK2/CycA kinase. In a 50 µl reaction, 50 ng of CDK2/CycA and increasing concentrations of substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)](http://www.bioprodhub.com/system/product_images/ak_products/1/sub_1/1854_7522_de_subst_jp_050928.jpg)
Figure 4. Peptide concentration dependence of CDK2/CycA kinase activity: DELFIA® data generated using Phospho-Rb (Ser807/811) Antibody #9308 to detect phosphorylation of substrate peptide (#1144) by CDK2/CycA kinase. In a 50 µl reaction, 50 ng of CDK2/CycA and increasing concentrations of substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)
![Figure 2. Time course of CDK2/CycA kinase activity: DELFIA® data generated using Phospho-Rb (Ser807/811) Antibody #9308 to detect phosphorylation of substrate peptide (#1144) by CDK2/CycA kinase. In a 50 µl reaction, 50 ng CDK2/CycA and 1.5 µM substrate peptide were used per reaction. (DELFIA® is a registered trademark of PerkinElmer, Inc.)](http://www.bioprodhub.com/system/product_images/ak_products/1/sub_1/1855_7522_de_time_jp_050928.jpg)
Figure 2. Time course of CDK2/CycA kinase activity: DELFIA® data generated using Phospho-Rb (Ser807/811) Antibody #9308 to detect phosphorylation of substrate peptide (#1144) by CDK2/CycA kinase. In a 50 µl reaction, 50 ng CDK2/CycA and 1.5 µM substrate peptide were used per reaction. (DELFIA® is a registered trademark of PerkinElmer, Inc.)