PathScan ® Total eIF2α Sandwich ELISA Kit
| Name | PathScan ® Total eIF2α Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7952 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human |
| Description | The PathScan ® Total eIF2α Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of eIF2α protein |
| Gene | EIF2S1 |
| Supplier Page | Shop |
Product images
Figure 2. The relationship between the protein concentration of lysates from Jurkat cells treated with calyculin A and pervanadate or LY294002 and the absorbance at 450 nm using PathScan ® Total eIF2α Sandwich ELISA Kit is shown.
Figure 1. Treatment of Jurkat cells with 100 nM Calyculin A #9902 and 1 mM pervanadate increases phosphorylation of eIF2α at Ser51, detected by the PathScan ® Phospho-eIF2α (Ser51) Sandwich ELISA Kit #7948, but does not affect the levels of total eIF2α detected by PathScan ® Total eIF2α Sandwich ELISA Kit #7952. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using eIF2α (L57A5) Mouse mAb #2103 (left panel) or Phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597 (right panel) are shown in the bottom figure.