Cell Cycle In-Cell ELISA Kit (Fluorescent)
| Name | Cell Cycle In-Cell ELISA Kit (Fluorescent) |
|---|---|
| Supplier | Abcam |
| Catalog | ab140363 |
| Category | ELISA Kit |
| Prices | $438.00 |
| Sizes | 1 x 96 tests |
| Assay Type | Cell-based (quantitative) |
| Sample Type | Adherent cells |
| Detection | Fluorescent |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Mouse, Human |
| Gene | CDK2 |
| Supplier Page | Shop |
Product images
Whole cell lysates from HeLa cells were analyzed by Western blot with the primary antibodies used in this assay kit. (A) Histone H3 pSer10 antibody: Lane 1 -Untreated, Lane 2- hydroxyurea = G1/S arrest, Lane 3- paclitaxel = G2/M arrest . (B) Cdk2 pTyr15 antibody: Lane 1 -Untreated,Lane 2-, thymidine = G1/S arrest, Lane 3- nocodazole = G2/M arrest.
Data shown is for 24 hour treatment with 1.6 – 5000 nM hydroxyurea. Cdk2 pTyr15 intensity increases with hydroxyurea treatment dose whereas Histone H3 pSer10 intensity decreases.
Data shown is for 24 hour treatment with 1 mM hydroxyurea, 333 nM paclitaxel and untreated (Control). (Normalized intensity is described in section 9.)