PARP1

Gene Symbol PARP1
Entrez Gene 142
Alt Symbol ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1
Species Human
Gene Type protein-coding
Description poly (ADP-ribose) polymerase 1
Other Description ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|ADP-ribosyltransferase diphtheria toxin-like 1|NAD(+) ADP-ribosyltransferase 1|poly (ADP-ribose) polymerase family, member 1|poly [ADP-ribose] polymerase 1|poly(ADP-ribose) polymerase|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase|poly[ADP-ribose] synthase 1
Swissprots B1ANJ4 P09874 Q8IUZ9
Accessions AAA51663 AAA60000 AAM75364 CAA34663 CAA39606 CBX74363 EAW69783 EAW69784 EAW69785 P09874 AF401218 AAL02174 AK125650 AK225654 AK303340 BAG64403 AK312339 BAG35260 BC008660 BC014206 AAH14206 BC018620 AAH18620 BC021045 BC037545 AAH37545 DB097441 DQ891636 ABM82562 DQ894826 ABM85752 J03473 AAB59447 M17081 AAA51599 M18112 AAA60137 M32721 AAA60155 NM_001618 NP_001609
Function Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. {ECO:0000269|PubMed:17177976, E
Subcellular Location Nucleus. Nucleus, nucleolus. Note=Localizes at sites of DNA damage.
Top Pathways Base excision repair

PathScan ® Cleaved PARP (Asp214) Sandwich ELISA Kit - 7262 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

PathScan ® Cleaved PARP (Asp214) Sandwich ELISA Antibody Pair - 7858 from Cell Signaling Technology

Category Antibody Pair

Cleaved PARP Human ELISA Kit - ab119690 from Abcam

Category ELISA Kit
Sensitivity 15 µg/ml
Range 8 µg/ml - 250 µg/ml
Figure 4B&C. Demonstration of assay specificity by inducing PARP cleavage with Staurosporine. (B) Western blot using the Cleaved PARP ELISA capture antibody. (C) Western blot using the Cleaved PARP ELISA detector antibody. The same extract preparations of vehicle-treated (lane1) or Staurosporine-treated (lane 2) HeLa cells, and of vehicle-treated (lane 3) or Staurosporine-treated (lane 4) Jurkat cells were used in both ELISA and Western blot analyses. The data demonstrate that the ELISA kit measures specifically the 89 kDa fragment of PARP-1. Note of complete cleavage of PARP in the Staurosporine-treated HeLa and Jurkat samples (in C).

Cleaved PARP Human In-Cell ELISA KIT (Colorimetric) - ab139410 from Abcam

Category ELISA Kit
Prices $438.00
Sizes 1 x 96 tests
Dynamic range of the ab139410 assay kit. HeLa cells were allowed attach overnight and treated for 4 hours with 1 µM staurosporine (ab120056). The data, presented as background-subtracted OD 450 nm values (mean +/-SEM), were obtained using this kit as described in the protocol.

Cleaved PARP Human In-Cell ELISA Kit (Fluorescent) - ab140362 from Abcam

Category ELISA Kit
Prices $438.00
Sizes 1 x 96 tests
Range 3000 cells/well - 100000 cells/well
HeLa cells, seeded at indicated densities, were allowed attach overnight and treated for 4 hours with drug vehicle (DMSO) or 1 µM staurosporine (ab120056) to induce apoptosis. The data (mean +/-SEM), presented as background-subtracted RFU values (A), or Janus green-normalized signals (B), were obtained using this kit as described in the protocol.

Cleaved PARP Human SimpleStep ELISA™ Kit - ab174441 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 5.8 µg/ml
Range 7.8 µg/ml - 500 µg/ml
Background-subtracted data values (mean +/- SD) are graphed.

Human PARP1 ELISA Kit (Sandwich ELISA) - LS-F8694 from LifeSpan Bioscience

Category ELISA Kit
Prices $650.00
Sizes 1 plate
Sensitivity 0.781 ng/ml
Range 0.781-50 ng/ml

Rat PARP1 ELISA Kit (Sandwich ELISA) - LS-F12265 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 78
Range 0.781-50 ng/ml

Human PARP1 ELISA Kit (Sandwich ELISA) - LS-F12266 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 0.625
Range 0.781-50 ng/ml

Rabbit anti-PARP1 Antibody, Affinity Purified - PLA0184 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

PARP Universal Colorimetric Assay Kit - 4677-096-K from R&D Systems

Category Assay Kit
Prices $489.00
Sizes 1 kit

HT PARP In Vivo Pharmacodynamic Assay II Kit - 4520-096-K from R&D Systems

Prices $1,250.00
Sizes 1 kit

PARP Enzyme High Specific Activity - 4668-500-01 from R&D Systems

Prices $935.00
Sizes 5000 un

PARP Enzyme High Specific Activity - 4668-100-01 from R&D Systems

Prices $269.00
Sizes 1000 un

Human PARP ELISA Kit - abx152650 from Abbexa

Category ELISA Kit
Sensitivity 0.25 ng/ml
Range 0.781 ng/ml - 50 ng/ml

Poly [ADP-ribose] polymerase 1, PARP1, Human, ELISA Kit - E0947h from ARP American Research Products

Category ELISA Kit
Prices $596.00
Sizes 96 t
Range 0.625-40 ng/mL

Poly ADP Ribose Polymerase (PARP), Human, ELISA Kit - SEA279Hu from ARP American Research Products

Category ELISA Kit
Prices $650.00
Sizes 96 t
Sensitivity 0.25ng/mL
Range 0.78 -50ng/mL

Poly ADP ribose polymerase, PARP, Human, ELISA Kit - CSB-E10705h from ARP American Research Products

Category ELISA Kit
Prices $550.00, $790.00, $791.00, $794.00
Sizes 48 t, 96 t, 5 x 96t, 10 x 96t
Sensitivity 3.9 pg/ml
Range 15.6 pg/ml-1000 pg/ml

Human PARP ELISA Kit - HP0031 from NeoScientific

Category ELISA Kit
Prices $495.00
Sizes 96 t

Anserine PARP ELISA Kit - AP0031 from NeoScientific

Category ELISA Kit
Prices $300.00, $480.00
Sizes 100 µl, 200 µl

Poly ADP Ribose Polymerase, ELISA Kit - MBS085965 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1ng/ml.
Range 0.5ng/ml-16ng/ml.

Poly ADP Ribose Polymerase, ELISA Kit - MBS091861 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1ng/ml.
Range 0.5ng/ml-16ng/ml.

Poly ADP Ribose Polymerase, ELISA Kit - MBS050919 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1ng/ml.
Range 0.5ng/ml-16ng/ml.

Poly ADP Ribose Polymerase, ELISA Kit - MBS057842 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1ng/ml.
Range 0.5ng/ml-16ng/ml.

Poly ADP Ribose Polymerase, ELISA Kit - MBS085246 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1ng/ml.
Range 0.5ng/ml-16ng/ml.
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