PPP2CA

Gene Symbol PPP2CA
Entrez Gene 5515
Alt Symbol PP2Ac, PP2CA, PP2Calpha, RP-C
Species Human
Gene Type protein-coding
Description protein phosphatase 2, catalytic subunit, alpha isozyme
Other Description PP2A-alpha|protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform|protein phosphatase 2A catalytic subunit, alpha isoform|replication protein C|serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform|serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Swissprots P67775
Accessions AAA36466 EAW62268 EAW62269 P67775 AB451240 BAG70054 AB451365 BAG70179 AK057424 BAG51913 AK097599 BAG53493 AV705728 BC000400 AAH00400 BC002657 AAH02657 BC019275 AAH19275 BC031696 AAH31696 BM786995 CR457417 CAG33698 J03804 AAB38019 X12646 CAA31176 NM_002715 NP_002706
Function PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. {ECO:0000250, ECO:0000269|PubMed:10801873, ECO:0000269|PubMed:22613722, ECO:0000269|PubMed:9920888}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:16541025}. Nucleus {ECO:0000269|PubMed:16541025}. Chromosome, centromere {ECO:0000269|PubMed:16541025}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:16541025}. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity). {ECO:0000250}.
Top Pathways Sphingolipid signaling pathway, Adrenergic signaling in cardiomyocytes, AMPK signaling pathway, Hippo signaling pathway, TGF-beta signaling pathway

PP2A C Subunit (52F8) Rabbit mAb - 2259 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 52F8
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using PP2A C (52F8D8) Rabbit mAb.

PP2A C Subunit Antibody - 2038 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells using PP2A C Subunit Antibody.

Nonmethylated PP2A C Subunit (4B7) Mouse mAb - 4957 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 4B7

PP2A-Cα (N-25) - sc-130237 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
PP2A-Cα (N-25): sc-130237. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining

PP2A-Cα/β (1D6) - sc-80665 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
PP2A-Cα/β (1D6): sc-80665. Western blot analysis of PP2A-C expression in Hep G2 (A), K-562 (B) and HeLa (C) whole cell lysates.

PP2A-Cα/β (C-1) - sc-376673 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PP2A-Cα/β (C-1): sc-376673. Western blot analysis of PP2A-Cα/β expression in MCF7 (A), T-47D (B) and KNRK (C) whole cell lysates.

PP2A-Cα/β (D-3) - sc-376824 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PP2A-Cα/β (D-3): sc-376824. Western blot analysis of PP2A-Cα/β expression in MCF7 (A) and T-47D (B) whole cell lysates.

PP2A-Cα/β (C-20) - sc-6110 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PP2A-Cα/β (C-20): sc-6110. Western blot analysis of PP2A-Cα/β expression in KNRK (A), MCF7 (B) and T-47D (C) whole cell lysates.

PP2A-Cα/β (FL-309) - sc-14020 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PP2A-Cα/β (FL-309): sc-14020. Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse uterus tissue (A) and immunofluorescence staining of methanol-fixed NIH/3T3 cells (B) showing cytoplasmic localization.

PP2A-Cα/β (G-4) - sc-166034 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PP2A-Cα/β (G-4): sc-166034. Western blot analysis of PP2A-Cβ expression in non-transfected 293T: sc-117752 (A), human PP2A-Cβ transfected 293T: sc-113470 (B) and MCF7 (C) whole cell lysates.

PP2A-Cα/β (0.T.118) - sc-56950 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
PP2A-Cα/β (0.T.118): sc-56950. Western blot analysis of PP2A-C expression in KNRK (A) and A-431 (B) whole cell lysates.

PP2A-Cα/β(7A6) - sc-81601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PP2A-Cα/β (7A6): sc-81601. Western blot analysis of PP2A-Cα/β expression in K-562 whole cell lysate.

demethylated-PP2A-C (4B7) - sc-13601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
demethylated-PP2A-C (4B7): sc-13601. Western blot analysis of demethylated-PP2A-C expression in Hep G2 (A), K-562 (B) and HeLa (C) whole cell lysates.

p-PP2A-Cα/β (F-8) - sc-271903 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PP2A-Cα/β phosphorylation in untreated (A,D), EGF treated (B,E) and EGF and Alkaline phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-PP2A-Cα/β (TF-8): sc-271903 (A,B,C) and PP2A-Cα/β (1D6): sc-80665 (D,E,F).

Anti-PP2A alpha + beta antibody - ab16450 from Abcam

Prices $370.00
Sizes 100 µl
Host Sheep
Clonality Polyclonal

Anti-PP2A alpha + beta antibody - ab135951 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PP2A alpha + beta antibody (ab135951) at 1/500 dilution + A549 cell extracts at 30 µg

Anti-PP2A alpha + beta antibody - ab1309 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-PP2A alpha + beta antibody (ab1309) at 0.05 µg/ml + Human Cerebellum lysates at 35 µgdeveloped using the ECL technique

Anti-PP2A alpha + beta antibody [Y119] - ab32141 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y119
Anti-PP2A alpha + beta antibody [Y119] (ab32141) at 1/10000 dilution + T47-D cell lysate

Anti-PP2A-alpha (methyl L309) antibody [2A10] - ab66597 from Abcam

Prices $400.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2A10
All lanes : Anti-PP2A-alpha (methyl L309) antibody [2A10] (ab66597) at 10 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PP2A-alpha (methyl L309) antibody [2A10] (HRP) - ab187948 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2A10

Anti-PP2A-alpha (phospho Y307) antibody - ab59222 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59222 (1/100 dilution) staining PP2A alpha in Human lung carcinoma tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunising peptide.ab59222 (1/100 dilution) staining PP2A alpha in human lung carcinoma tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunising peptide.

Anti-PP2A-alpha (phospho Y307) antibody [E155] - ab32104 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E155
All lanes : Anti-PP2A-alpha (phospho Y307) antibody [E155] (ab32104) at 1/5000 dilutionLane 1 : untreated A431 cell lysateLane 2 : EGF treated A431 cell lysate (the specific EGF concentration and incubation time is confidential information)

Anti-PP2A-alpha (phospho Y307) antibody [E155] (Biotin) - ab197706 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E155
IHC image of PP2A alpha staining in a section of formalin-fixed paraffin-embedded normal human pancreas*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab197706 at 1/100 dilution for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-PP2A-alpha (phospho Y307) antibody [E155] (HRP) - ab196979 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E155
Anti-PP2A-alpha (phospho Y307) antibody [E155] (HRP) (ab196979) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PP2A-alpha antibody - ab106262 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PP2A-alpha antibody (ab106262) at 0.5 µg/mlLane 1 : Rat kidney tissue LysateLane 2 : Rat Liver tissue LysateLane 3 : Human Placenta tissue LysateLane 4 : Mouse Kidney tissue LysateLane 5 : A431 Whole Cell LysateLane 6 : HeLa Whole Cell LysateLane 7 : 293T Whole Cell LysateLane 8 : NIH Whole Cell LysateLane 9 : PC12 Whole Cell LysateLysates/proteins at 40 µg per lane.
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