TAF1

Gene Symbol TAF1
Entrez Gene 6872
Alt Symbol BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, N-TAF1, NSCL2, OF, P250, TAF(II)250, TAF2A, TAFII-250, TAFII250, XDP
Species Human
Gene Type protein-coding
Description TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
Other Description TBP-associated factor 250 kDa|cell cycle gene 1 protein|cell cycle, G1 phase defect|complementation of cell cycle block, G1-to-S|transcription factor TFIID p250 polypeptide|transcription initiation factor TFIID subunit 1
Swissprots Q70T03 Q70T00 Q59FZ3 Q6IUZ1 A5CVC8 A5CVC9 P21675 Q70Q86 Q70T01 A5CVD0 Q70T02 A5CVD1 B1Q2X3 Q70Q87
Accessions AAT38105 EAX05291 EAX05292 EAX05293 EAX05294 EAX05295 P21675 AB209316 BAD92553 AB300418 BAG15901 AJ549245 CAD70488 AJ549246 CAD70489 AJ549247 CAD70490 AJ549248 CAD70491 AJ549249 CAD70492 AJ549250 CAD70493 AJ555148 CAD87527 AJ555149 CAD87528 AK310201 AM711892 CAM98555 AM711893 CAM98556 AM711894 CAM98557 AM711895 CAM98558 AW937788 BC172427 AAI72427 BP284702 BQ027170 D90359 BAA14374 M73444 R66536 X07024 CAA30073 XM_005262295 XP_005262352 XM_005262296 XP_005262353 XM_005262297 XP_005262354 XM_005262300 XP_005262357 XM_006724682 XP_006724745 XM_011531016 XP_011529318 XR_938407 NM_001286074 NP_001273003 NM_004606 NP_004597 NM_138923 NP_620278 NR_104387 NR_104388 NR_104389 NR_104390 NR_104391 NR_104392 NR_104393 NR_104394 NR_104395 NR_104396
Function Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA- binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4. {ECO:0000269|PubMed:11278496, ECO:0000269|PubMed:15053879, ECO:0000269|PubMed:2038334, ECO:0000269|PubMed:8450888, ECO:0000269|PubMed:8625415, ECO:0000269|PubMed:9660973, ECO:0000269|PubMed:9858607}.
Subcellular Location Nucleus {ECO:0000269|PubMed:2038334}.
Top Pathways Basal transcription factors

TAF1 (D6J8B) Rabbit mAb - 12781 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6J8B
Western blot analysis of extracts from Hep G2, SW620 and U-2 OS cells using TAF1 (D6J8B) Rabbit mAb.

TAF II p250 (6B3) - sc-735 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TAF II p250 (6B3): sc-735. Western blot analysis of hTAF II p250 expression in HeLa (A) and A-431 (B) whole cell lysates.

TAF II p250 (A-10) - sc-393981 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TAF II p250 (A-10): sc-393981. Western blot analysis of TAF II p250 expression in HeLa whole cell lysate (A) and K-562 nuclear extract (B).

TAF II p250 (C-19) - sc-46648 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TAF II p250 (L-20) - sc-17134 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TAF II p250 (L-20): sc-17134. Western blot analysis of TAF II p250 expression in K-562 nuclear extract.

TAF II p250 (N-12) - sc-46649 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-KAT4 / TBP Associated Factor 1 antibody - ab75656 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-KAT4 / TBP Associated Factor 1 antibody (ab75656) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-KAT4 / TBP Associated Factor 1 antibody - ab51540 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KAT4 / TBP Associated Factor 1 antibody [0.T.145] - ab14211 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 0.T.145

Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)] - ab168346 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7145(2)
All lanes : Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)] (ab168346) at 1/1000 dilutionLane 1 : 293T lysateLane 2 : SH-SY5Y lysateLane 3 : HepG2 lysateLane 4 : Human fetal brain lysateLane 5 : Jurkat lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade - ab28450 from Abcam

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

Anti-KAT4 / TBP Associated Factor 1 antibody - ChIP Grade - ab85533 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

Anti-TAF1 antibody - ab188427 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence staining of TAF1 in HeLa cells with ab188427 at a 1/5000 dilution. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100.

Anti-TAF1 Antibody (aa1379-1636, Biotin) - LS-C300033 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TAF1 Antibody (aa1379-1636, FITC) - LS-C302292 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TAF1 Antibody (C-Terminus) - LS-C29842 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TAF1 antibody LS-C29842 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-TAF1 Antibody (aa1800-1849) - LS-C101394 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TAF1 antibody LS-C101394 Western blot of Fetal liver lysate.

Anti-TAF1 Antibody (aa1860-1893) - LS-C325553 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TAF1 Antibody (clone 1E11) - LS-C198299 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1E11
Detection limit for recombinant GST tagged TAF1 is approximately 0.1 ng/ml as a capture antibody.

Anti-TAF1 Antibody - LS-C347721 from LifeSpan Bioscience

Prices $345.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-TAF1 Antibody (aa1863-1893) - LS-C161684 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
TAF1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The TAF1 antibody detected the TAF1 protein (arrow).

Anti-TAF1 Antibody (aa1750-1800) - LS-C290174 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human TAF1 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TAF1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-TAF1 Antibody (aa1379-1636) - LS-C296574 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant TAF1.

Anti-TAF1 Antibody (aa1131-1180) IHC-plus™ - LS-B8406 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TAF1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8406 dilution 5 ug/ml.

Anti-TAF1 Antibody IHC-plus™ - LS-B1907 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-TAF1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1907 dilution 1:50.
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