PTK2B

Gene Symbol PTK2B
Entrez Gene 2185
Alt Symbol CADTK, CAKB, FADK2, FAK2, PKB, PTK, PYK2, RAFTK
Species Human
Gene Type protein-coding
Description protein tyrosine kinase 2 beta
Other Description CAK-beta|FADK 2|PTK2B protein tyrosine kinase 2 beta|calcium-dependent tyrosine kinase|calcium-regulated non-receptor proline-rich tyrosine kinase|cell adhesion kinase beta|focal adhesion kinase 2|proline-rich tyrosine kinase 2|protein kinase B|protein-tyrosine kinase 2-beta|related adhesion focal tyrosine kinase
Swissprots Q6PID4 Q14290 Q13475 Q16709 D3DST0 Q14289
Accessions EAW63553 EAW63554 EAW63555 EAW63556 Q14289 AB209085 BAD92322 AK128371 BAC87404 AL120380 AW130888 AY429564 BC036651 AAH36651 BC042599 AAH42599 BF796371 BM712234 BQ009032 D45853 BAA08289 DA773624 DQ590775 L49207 AAB47217 U33284 AAC50203 U43522 AAC05330 XM_005273447 XP_005273504 XM_005273448 XP_005273505 XM_011544441 XP_011542743 XM_011544442 XP_011542744 NM_004103 NP_004094 NM_173174 NP_775266 NM_173175 NP_775267 NM_173176 NP_775268
Function Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T- cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activati
Subcellular Location Cytoplasm. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Cell projection, lamellipodium. Cytoplasm, cell cortex. Nucleus. Note=Interaction with NPHP1 induces the membrane-association of the kinase. Colocalizes with integrins at the cell periphery.
Tissue Specificity Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes. {ECO:0000269|PubMed:7544443, ECO:0000269|PubMed:9545257}.
Top Pathways Natural killer cell mediated cytotoxicity, Leukocyte transendothelial migration, GnRH signaling pathway, Calcium signaling pathway, Chemokine signaling pathway

Phospho-Pyk2 (Tyr402) Antibody - 3291 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Immunoprecipitation of Pyk2 from Ramos cells, untreated, H 2 O 2 -treated, or Pervanadate-treated, using Pyk2 Antibody #3292, followed by Western blot with Phospho-Pyk2 (Tyr402) Antibody (upper), or Pyk2 Antibody #3292 (lower).

Pyk2 (5E2) Mouse mAb - 3480 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 5E2
Western blot analysis of extracts from various cell lines using Pyk2 (5E2) Mouse mAb.

Pyk2 (H364) Antibody - 3090 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat and Ramos cells using Pyk2 (H364) Antibody.

Pyk2 Antibody - 3292 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SKW6.4 (lane 1), Jurkat (lane 2), Namalwa (lane 3) and LS 102.9 (lane 4) cells using Pyk2 Antibody.

PYK2 (E-3) - sc-393181 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PYK2 (E-3): sc-393181. Western blot analysis of PYK2 expression in non-transfected 293T: sc-117752 (A), human PYK2 transfected 293T: sc-115595 (B) and Ramos (C) whole cell lysates.

PYK2 (D-1) - sc-365201 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PYK2 (D-1): sc-365201. Western blot analysis of PYK2 expression in Ramos whole cell lysate.

PYK2 (C-19) - sc-1515 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PYK2 (C-19): sc-1515. Western blot analysis of PYK2 expression in mouse brain tissue extract.

PYK2 (F-6) - sc-74539 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PYK2 (F-6): sc-74539. Western blot analysis of PYK2 expression in non-transfected 293T: sc-117752 (A), human PYK2 transfected 293T: sc-115595 (B) and Ramos (C) whole cell lysates.

PYK2 (H-102) - sc-9019 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PYK2 (H-102): sc-9019. Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.

PYK2 (5E2D5) - sc-130077 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PYK2 (5E2D5): sc-130077. Western blot analysis of PYK2 expression in Raji (A), PMA stimulated THP-1 (B), Jurkat (C) and Ramos (D) whole cell lysates.

PYK2 (N-19) - sc-1514 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PYK2 (N-19): sc-1514. Western blot analysis of PYK2 expression in PC-12 whole cell lysate.

PYK2 (Y-129) - sc-100379 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
PYK2 (Y-129): sc-100379. Western blot analysis of PYK2 expression in non-transfected 293T: sc-117752 (A), human PYK2 transfected 293T: sc-115595 (B) and Ramos (C) whole cell lysates.

p-PYK2 (13.Tyr 402) - sc-293142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PYK2 phosphorylation in non-transfected: sc-117752 (A,D), untreated human PYK2 transfected: sc-115595 (B,E) and lambda protein phosphatase (sc-200312A) treated human PYK2 transfected: sc-115595 (C,F) 293T whole cell lysates. Antibodies tested include p-PYK2 (13.Tyr 402): sc-293142 (A,B,C) and PYK2 (N-19): sc-1514 (D,E,F).

p-PYK2 (Tyr 579) - sc-16822 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-PYK2 (Tyr 579): sc-16822. Western blot analysis of PYK2 phosphorylation in non-transfected: sc-117752 (A) and human PYK2 transfected: sc-115595 (B) 293T whole cell lysates.

p-PYK2 (Tyr 579/Tyr 580) - sc-16824 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-PYK2: sc-16824. Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear and cytoplasmic staining of glandular cells.

p-PYK2 (Tyr 580) - sc-16823 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-PYK2 (Tyr 580): sc-16823. Western blot analysis of PYK2 phosphorylation in non-transfected: sc-117752 (A) and human PYK2 transfected: sc-115595 (B) 293T whole cell lysates.

p-PYK2 (Tyr 881) - sc-16825 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of PYK2 phosphorylation in non-transfected: sc-117752 (A, D), untreated human PYK2 transfected: sc-115595 (B, E) and lambda protein phosphatase treated human PYK2 transfected: sc-115595 (C, F) 293T whole cell lysates. Antibodies tested include p-PYK2 (Tyr 881)-R: sc-16825-R (A, B, C) and PYK2 (N-19): sc-1514 (D, E, F).

p-PYK2 (14F6) - sc-81512 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-PYK2 (14F6): sc-81512. Western blot analysis of PYK2 phosphorylation in non-transfected: sc-117752 (A) and human PYK2 transfected: sc-115595 (B) 293T whole cell lysates.

p-PYK2 (Tyr 402) - sc-101790 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-PYK2 (Tyr 402): sc-101790. Western blot analysis of phosphorylated PYK2 expression in untreated (A) and PMA-treated (B) Jurkat whole cell lysates.

Anti-PYK2 (phospho Y402) antibody - ab131543 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PYK2 (phospho Y402) antibody (ab131543) at 1/500 dilutionLane 1 : Jurkat cell lysate, untreatedLane 2 : Jurkat cell lysate, treated with PMA

Anti-PYK2 (phospho Y579) antibody - ab78209 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PYK2 (phospho Y579) antibody (ab78209) at 1/500 dilutionLane 1 : Extracts from 3T3 cellsLane 2 : Extracts from 3T3 cells plus 5µg immunizing peptideLysates/proteins at 5 µg per lane.

Anti-PYK2 (phospho Y579) antibody - ab193674 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PYK2 (phospho Y579) antibody (ab193674) at 1/500 dilutionLane 1 : Extracts from 3T3 cells treated with antigen-specific peptideLane 2 : Extracts from 3T3 cellsLane 3 : Extracts from HepG2 cells

Anti-PYK2 (phospho Y579) antibody - ab4805 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PYK2 (phospho Y580) antibody - ab52209 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image shows human brain tissue stained with ab52209 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.

Anti-PYK2 (phospho Y881) antibody - ab53127 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PYK2 (phospho Y881) antibody (ab53127) at 1/300 dilutionLane 1 : mouse brain extractLane 2 : mouse brain extract with immunising peptide
Search more