ATG12

Gene Symbol ATG12
Entrez Gene 9140
Alt Symbol APG12, APG12L, FBR93, HAPG12
Species Human
Gene Type protein-coding
Description autophagy related 12
Other Description ATG12 autophagy related 12 homolog|Apg12 (autophagy, yeast) homolog|ubiquitin-like protein ATG12
Swissprots O94817 Q6PJV2
Accessions EAW48954 EAW48955 EAW48956 O94817 AB002440 AB002449 AB017507 BAA36493 AK098251 AK313320 BAG36125 AL161968 BC011033 AAH11033 BC012266 AAH12266 BM697565 BP335591 BX327373 CB995198 CN400030 CR978239 DA005347 DB048378 DB530852 EU554057 ACD74941 NR_073604 NM_001277783 NP_001264712 NM_004707 NP_004698 NR_033362 NR_033363 NR_073603
Function Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. {ECO:0000269|PubMed:12207896, ECO:0000269|PubMed:17709747, ECO:0000269|PubMed:17999726, ECO:0000269|PubMed:19074260, ECO:0000269|PubMed:19164948, ECO:0000269|PubMed:19666601, ECO:0000269|PubMed:23202584}.
Subcellular Location Cytoplasm {ECO:0000250}. Preautophagosomal structure membrane {ECO:0000269|PubMed:22342342}; Peripheral membrane protein {ECO:0000269|PubMed:22342342}. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:9852036}.
Top Pathways RIG-I-like receptor signaling pathway, Regulation of autophagy

Atg12 Antibody (Human Specific) - 2010 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Atg12 Antibody (Human Specific).

Atg12 (D88H11) Rabbit mAb - 4180 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D88H11
Western blot analysis of extracts from various cell lines using Atg12 (D88H11) Rabbit mAb.

Atg12 (C-6) - sc-271688 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Atg12 (C-6): sc-271688. Western blot analysis of Atg12 expression in HCT 116 whole cell lysate.

Atg12 (C-18) - sc-70128 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Atg12 (FL-140) - sc-68884 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Atg12 (FL-140): sc-68884. Western blot analysis of Atg12 expression in Hep G2 whole cell lysate.

Atg12 (P-19) - sc-70130 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Atg12 (P-19): sc-70130. Western blot analysis of Atg12 expression in non-transfected: sc-110760 (A) and human Atg12 transfected: sc-113050 (B) 293 whole cell lysates.

Anti-Apg12 antibody - ab155589 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Apg12 antibody (ab155589) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

Anti-Apg12 antibody - ab64213 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Apg12 antibody - ab69456 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Apg12 antibody - ab56465 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56465 Apg12 antibody at 1ug/ml.

Anti-Apg12 antibody - ab172232 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Apg12 antibody (ab172232) at 1 µg/mlLane 1 : Apg2 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateSecondaryGoat Anti-Mouse IgG at 1/2500 dilution

Anti-Apg12 antibody [EPR4799] - Autophagosome Marker - ab109492 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4799
Overlay histogram showing HepG2 cells stained with ab109492 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109492, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (Alexa Fluor® 488) - ab185028 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4799
Overlay histogram showing HeLa cells stained with ab185028 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab185028, 1/500 dilution) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Apg12 antibody [EPR4800] - ab109491 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4800
All lanes : Anti-Apg12 antibody [EPR4800] (ab109491) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : HepG2 lysateLane 3 : Raji lysateLysates/proteins at 10 µg per lane.

Anti-APG12 / ATG12 Antibody (aa84-101) - LS-C47479 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-APG12 / ATG12 Antibody - LS-C59542 from LifeSpan Bioscience

Prices $395.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-APG12 / ATG12 Antibody (N-Terminus) - LS-C69121 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-APG12 / ATG12 Antibody (N-Terminus) - LS-C69126 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-APG12 / ATG12 Antibody (N-Terminus) - LS-C69127 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-APG12 / ATG12 Antibody - LS-C69128 from LifeSpan Bioscience

Prices $485.00
Sizes 250 µg
Host Rabbit
Clonality Polyclonal

Anti-APG12 / ATG12 Antibody (N-Terminus) - LS-C69122 from LifeSpan Bioscience

Prices $415.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-APG12 / ATG12 Antibody - LS-C93899 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Sheep
Clonality Polyclonal

Anti-APG12 / ATG12 Antibody - LS-C125098 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-APG12 / ATG12 Antibody - LS-C126828 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal

Anti-APG12 / ATG12 Antibody - LS-C125099 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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