CAD

Gene Symbol CAD
Entrez Gene 790
Alt Symbol -
Species Human
Gene Type protein-coding
Description carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Other Description CAD protein|CAD trifunctional protein|multifunctional protein CAD
Swissprots P27708 Q6P0Q0 Q96CK3 D6W552
Accessions AAA51907 AAX93165 AAY24293 EAX00612 EAX00613 EAX00614 P27708 AB621827 BAK64162 AK226105 BC014178 AAH14178 BC065510 AAH65510 BG766243 BM927624 BQ447516 BU541191 BU558264 D78586 BAA11423 XM_005264555 XP_005264612 XM_005264556 XP_005264613 XM_005264557 XP_005264614 XM_005264558 XP_005264615 XM_006712101 XP_006712164 NM_001306079 NP_001293008 NM_004341 NP_004332
Function This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase). {ECO:0000269|PubMed:24332717}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:15890648}. Nucleus {ECO:0000269|PubMed:15890648}. Note=Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.
Top Pathways Alanine, aspartate and glutamate metabolism, Pyrimidine metabolism

Phospho-CAD (Ser1859) Antibody - 12662 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells untreated (-) and HeLa cells, untreated (-) or treated with Rapamycin #9904 (10 nM, 16 hr; +), using Phospho-CAD (Ser1859) Antibody (upper) or CAD Antibody #11933 (lower).

CAD Antibody - 11933 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SW620, MEF, and C6 cells using CAD Antibody.

p-CPS2 (F-4) - sc-377559 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of CPS2 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (A,C) HeLa nuclear extracts. Antibodies tested include p-CPS2 (F-4): sc-377559 (A,B) and CPS2 (F-6): sc-376072 (C,D).

CPS2 (F-6) - sc-376072 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CPS2 (F-6): sc-376072. Western blot analysis of CPS2 expression in Y79 whole cell lysate.

CPS2 (C-17) - sc-10522 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CPS2 (C-17): sc-10522. Western blot analysis of CPS2 expression in non-transfected: sc-117752 (A) and human CPS2 transfected: sc-116936 (B) 293T whole cell lysates.

CPS2 (H-180) - sc-30061 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

CPS2 (K-16) - sc-10521 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CPS2 (N-20) - sc-10519 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-CPS2 (Thr 456) - sc-12964 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of CPS2 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (A,C) HeLa nuclear extracts. Antibodies tested include p-CPS2 (Thr 456)-R: sc-12964-R (A,B) and CPS2 (F-6): sc-376072 (C,D).

Anti-CAD (phospho T456) antibody - ab111837 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111837 at 1/50 dilution staining CAD in paraffin-embedded Human Colon carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-CAD (phospho T456) antibody - ab194534 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling CAD (phospho T456) with ab194534 at 1/50 dilution. The image on the right has been pre-incubated with the blocking peptide.

Anti-CAD antibody - ab97340 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab97340 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CAD antibody - ab99311 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CAD with ab99311 at 1/200 (1µg/ml). Detection: DAB.

Anti-CAD antibody - ab99312 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CAD with ab99312 at 1/200 (1µg/ml). Detection: DAB.

Anti-CAD antibody - ab99313 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CAD with ab99313 at 1/200 (1µg/ml). Detection: DAB.

Anti-CAD antibody - ab84474 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma, using 1/250 ab84474 followed by DAB staining.

Anti-CAD antibody [EP710Y] - ab40800 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP710Y
Anti-CAD antibody [EP710Y] (ab40800) at 1/1000 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902) at 10 µg

Anti-CAD antibody [EP711Y] - ab40765 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP711Y
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab40765 at 1/250 dilution.

Anti-CAD Antibody IHC-plus™ - LS-B4205 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-CAD antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4205 concentration 10 ug/ml.

Anti-CAD Antibody (N-Terminus) IHC-plus™ - LS-B11936 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-CAD Antibody (aa787-816, Biotin) - LS-C253291 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CAD Antibody (aa787-816, FITC) - LS-C253292 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CAD Antibody (aa787-816, Azide-free, HRP) - LS-C253293 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CAD Antibody (aa422-471) - LS-C198710 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human colon carcinoma tissue, using CAD Antibody. The sample on the right was incubated with synthetic peptide.

Anti-CAD Antibody (phospho-Thr456) - LS-C198711 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human colon carcinoma, using CAD (Phospho-Thr456) Antibody. The sample on the right was incubated with synthetic peptide.
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