TOP3B

Gene Symbol TOP3B
Entrez Gene 8940
Alt Symbol TOP3B1
Species Human
Gene Type protein-coding
Description topoisomerase (DNA) III beta
Other Description DNA topoisomerase 3-beta-1|DNA topoisomerase III beta-1|topoisomerase III beta
Swissprots A0M8Q3 O95985 Q9BUP5
Accessions BAA20009 EAW59487 EAW59488 EAW59489 EAW59490 EAW59491 O95985 AF017146 AAD01614 AF053082 AAD15791 AF125216 AAD29670 AK096695 BAG53351 AK290997 BAF83686 AK304681 BAG65452 AK304709 BAG65474 AL833505 BC002432 AAH02432 BQ270362 CR456596 CAG30482 CU013196 CAK54627 CU013484 CAK54926 DA025312 XM_005261811 XP_005261868 XM_005261813 XP_005261870 XM_006724349 XP_006724412 XM_006724350 XP_006724413 XM_011530482 XP_011528784 XM_011530483 XP_011528785 XM_011530484 XP_011528786 XR_244395 XR_937945 XR_937946 NM_001282112 NP_001269041 NM_001282113 NP_001269042 NM_003935 NP_003926
Function Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity. {ECO:0000250}.
Tissue Specificity Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas. {ECO:0000269|PubMed:9927731}.
Top Pathways Homologous recombination, Fanconi anemia pathway

Topo IIIβ-1 (B-10) - sc-137238 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo IIIβ-1 (B-10): sc-137238. Western blot analysis of Topo IIIβ expression in non-transfected 293T: sc-117752 (A), mouse Topo IIIβ transfected 293T: sc-124216 (B), K-562 (C) and IMR-32 (D) whole cell lysates.

Topo IIIβ-1 (E-13) - sc-11266 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Topo IIIβ-1 (E-13): sc-11266. Western blot analysis of Topo IIIβ expression in non-transfected: sc-117752 (A) and mouse Topo IIIβ transfected: sc-124216 (B) 293T whole cell lysates.

Topo IIIβ-1 (H-300) - sc-13061 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Topo IIIβ-1 (H-300): sc-13061. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in glomeruli and tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Topo IIIβ-1,2,3 (D-16) - sc-11264 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Topo IIIβ-1,2,3 (D-16): sc-11264. Western blot analysis of Topo IIIβ expression in non-transfected: sc-117752 (A) and mouse Topo IIIβ transfected: sc-124216 (B) 293T whole cell lysates.

Topo IIIβ-2 (H-20) - sc-11268 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Topo IIIβ-1,2,3 (L-17) - sc-11265 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Topo III-1,2,3 (L-17): sc-11265. Western blot analysis of Topo III-1,2,3 expression in NTERA-2 cl.D1 (A), NIH/3T3 (B) and HeLa (C) whole cell lysates.

Anti-TOP3B antibody - ab129927 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-TOP3B antibody (ab129927) at 1/100 dilution + Mouse bladder tissue lysates at 35 µg

Anti-TOP3B antibody - ab65005 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TOP3B antibody (ab65005) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : COLO205 cell extractLane 3 : HUVEC extractLane 4 : HUVEC extract with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-TOP3B antibody - ab56445 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-TOP3B antibody [EP7779] - C-terminal - ab183520 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP7779
All lanes : Anti-TOP3B antibody [EP7779] - C-terminal (ab183520) at 1/2000 dilutionLane 1 : K562 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-TOP3B Antibody (aa826-854, APC) - LS-C224382 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOP3B Antibody (aa826-854, AP) - LS-C224381 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOP3B Antibody (aa826-854, Biotin) - LS-C224383 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOP3B Antibody (aa826-854, FITC) - LS-C224384 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOP3B Antibody (aa826-854, Azide-free, HRP) - LS-C224385 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOP3B Antibody (aa826-854, PE) - LS-C224386 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOP3B Antibody (aa825-854) - LS-C325863 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOP3B Antibody (aa1-863, Azide-free) - LS-C328914 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-TOP3B Antibody (Gln84) - LS-C176718 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of Topo III-1 (Q84) pAb in extracts from HepG2 cells.

Anti-TOP3B Antibody (aa51-100) - LS-C118743 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HepG2/COLO205/HUVEC cells, using TOP3B Antibody. The lane on the right is treated with the synthesized peptide.

Anti-TOP3B Antibody (aa826-854) - LS-C160117 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
TOP3B Antibody western blot of mouse bladder tissue lysates (35 ug/lane). The TOP3B antibody detected the TOP3B protein (arrow).

Topo III beta Antibody (4F11) - H00008940-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4F11

Topo III beta Antibody - NB100-92303 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Topo III beta Antibody [NB100-92303] - Extracts from HepG2 cells.

Topo III beta Antibody (4H7) - H00008940-M05 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4H7

Topo III beta Antibody (1H4) - H00008940-M04 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1H4
Western Blot: Topo III beta Antibody (1H4) [H00008940-M04] - Analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M04), clone 1H4. Lane 1: TOP3B transfected lysatE (96.662 KDa). Lane 2: Non-transfected lysate.
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