CHST3

Gene Symbol CHST3
Entrez Gene 9469
Alt Symbol C6ST, C6ST1, HSD
Species Human
Gene Type protein-coding
Description carbohydrate (chondroitin 6) sulfotransferase 3
Other Description C6ST-1|GST-0|carbohydrate sulfotransferase 3|chondroitin 6-O-sulfotransferase 1|galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0
Swissprots O75099 Q52M30 Q7LGC8
Accessions EAW54440 Q7LGC8 AB012192 BAA32576 AB017915 BAA36348 AI041015 AK025251 BC033183 BC093690 AAH93690 BC104856 AAI04857 BG325090 DA223095 DB289525 XM_006718075 XP_006718138 XM_011540369 XP_011538671 NM_004273 NP_004264
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N- acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen. {ECO:0000269|PubMed:9714738, ECO:0000269|PubMed:9883891}.
Subcellular Location Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.
Tissue Specificity Widely expressed in adult tissues. Expressed in heart, placenta, skeletal muscle and pancreas. Also expressed in various immune tissues such as spleen, lymph node, thymus and appendix. {ECO:0000269|PubMed:9714738}.
Top Pathways Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate

C6ST-1 (G-9) - sc-271696 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
C6ST-1 (G-9): sc-271696. Western blot analysis of C6ST-1 expression in MIA PaCa-2 whole cell lysate.

C6ST-1 (H-110) - sc-98917 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
C6ST-1 (H-110): sc-98917. Western blot analysis of C6ST-1 expression in human skeletal muscle (A), mouse kidney (B) and mouse heart (C) tissue extracts.

C6ST-1 (A-8) - sc-271961 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
C6ST-1 (A-8): sc-271961. Western blot analysis of C6ST-1 expression in PC-12 whole cell lysate.

C6ST-1 (E-15) - sc-48973 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

C6ST-1 (A-15) - sc-48971 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

C6ST-1 (N-12) - sc-48974 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CHST3 antibody - ab107093 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CHST3 antibody (ab107093) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

Anti-CHST3 antibody - ab56606 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CHST3 antibody [EPR15789] - ab193359 from Abcam

Prices $355.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15789
All lanes : Anti-CHST3 antibody [EPR15789] (ab193359) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-CHST3 Antibody (aa312-412, clone 10B2239, Azide-free) - LS-C183500 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10B2239

Anti-CHST3 Antibody (aa32-62, AP) - LS-C233831 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST3 Antibody (aa32-62, APC) - LS-C233832 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST3 Antibody (aa32-62, Biotin) - LS-C233833 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST3 Antibody (aa32-62, FITC) - LS-C233835 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST3 Antibody (aa32-62, PE) - LS-C233836 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST3 Antibody (aa32-62, Azide-free, HRP) - LS-C249672 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST3 Antibody (aa312-411) - LS-C307795 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CHST3 Antibody (aa32-62) - LS-C320384 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST3 Antibody (aa32-62) - LS-C168763 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
CHST3 antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Carbohydrate Sulfotransferase 3/CHST3 Antibody - NBP2-31611 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: Carbohydrate sulfotransferase 3 Antibody [NBP2-31611] - Staining of human placenta shows strong cytoplasmic positivity in decidua cells.

Carbohydrate Sulfotransferase 3/CHST3 Antibody (799011) - MAB7450 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 799011
Carbohydrate Sulfotransferase 3/CHST3 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Carbohydrate Sulfotransferase 3/CHST3 Monoclonal Antibody (Catalog # MAB7450) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of endocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Carbohydrate Sulfotransferase 3/CHST3 Antibody (1C4) - H00009469-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C4

Carbohydrate Sulfotransferase 3/CHST3 Antibody - H00009469-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

ANTI-CHST3(N-TERMINAL) antibody produced in rabbit - SAB1306994 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CHST3 (N-term): <B>Cat. No. SAB1306994</B>: CHST3 Antibody (N-term) western blot analysis in Mouse spleen and lung tissue lysates (35 μg/lane).

Monoclonal Anti-CHST3 antibody produced in mouse - SAB1401475 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D3
<B>Immunofluorescence</B><BR/>Monoclonal Anti-CHST3: <B>Cat. No. SAB1401475</B>: Antibody concentration: 10 μg/mL using HeLa cell.
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