RAC1

Gene Symbol RAC1
Entrez Gene 5879
Alt Symbol MIG5, Rac-1, TC-25, p21-Rac1
Species Human
Gene Type protein-coding
Description ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Other Description cell migration-inducing gene 5 protein|ras-like protein TC25|ras-related C3 botulinum toxin substrate 1
Swissprots Q9BTB4 Q5JAA8 Q3Y4D3 P15154 P63000 O95501
Accessions AAB22206 AAS07511 AAS07512 AAZ80485 CAA10733 CAB53579 CAI61762 CAI61874 EAL23718 EAL23719 EAL23720 EAW55039 EAW55040 EAW55041 EAW55042 EAW55043 EAW55044 P63000 AB209410 BAD92647 AB451238 BAG70052 AB451363 BAG70177 AF136373 AAD30547 AF498964 AAM21111 AJ132694 CAA10732 AK054993 BAG51447 AK122742 AK291806 BAF84495 AK291815 BAF84504 AY279384 AAQ16632 BC004247 AAH04247 BC050687 AAH50687 BC107748 AAI07749 BG437715 BQ689587 BT007121 AAP35785 D25274 EU831965 ACE87406 EU832058 ACE86720 EU832640 ACE87731 EU832717 ACE87049 M29870 AAA36537 M31467 AAA36544 NM_006908 NP_008839 NM_018890 NP_061485 NM_198829
Function Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent ph
Subcellular Location Cell membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Melanosome {ECO:0000269|PubMed:17081065, ECO:0000269|PubMed:19948726, ECO:0000269|PubMed:21693584}. Cytoplasm {ECO:0000250}. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity). {ECO:0000250}.
Tissue Specificity Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
Top Pathways Wnt signaling pathway, Sphingolipid signaling pathway, Osteoclast differentiation, Rap1 signaling pathway, Choline metabolism in cancer

Rac1/2/3 Antibody - 2465 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS, C6, NIH/3T3 and HEK293 cells, using Rac1/2/3 Antibody.

Rac1/2/3 (L129) Antibody - 2467 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Rac 1 (C-11) - sc-95 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rac 1 (C-11): sc-95. Western blot analysis of human recombinant Rac 1 fusion protein.

Rac 1 (C-14) - sc-217 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rac 1 (C-14): sc-217. Western blot analysis of human recombinant Rac 1.

Rac 1 (T-17) - sc-6084 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rac 1 (T-17): sc-6084. Western blot analysis of Rac 1 expression in mouse embryo tissue extract.

Rac 1 (H-61) - sc-366230 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rac 1 (H-61): sc-366230. Western blot analysis of Rac 1 expression in mouse brain tissue extract.

Rac 3 (F-20) - sc-16698 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rac 3 (F-20): sc-16698. Western blot analysis of Rac 3 expression in C6 whole cell lysate.

Rac 1/2/3/4 (S-18) - sc-34259 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rac 1/2/3/4 (S-18): sc-34259. Western blot analysis of Rac 1/2/3/4 expression in MIA PaCa-2 (A), PC-3 (B) and T84 (C) whole cell lysates.

Rac 1/2/3/4 (E-20) - sc-34260 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rac 1/2/3/4 (E-20): sc-34260. Western blot analysis of Rac expression in non-transfected: sc-117752 (A) and human Rac transfected: sc-172574 (B) 293T whole cell lysates.

p-Rac 1 (Ser 71) - sc-135641 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Rac 1 (Ser 71): sc-135641. Western blot analysis of phosphorylated Rac 1 expression in untreated (A) and PMA-treated (B) 293 cell extracts.

Anti-RAC antibody [23A8] - ab13048 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 23A8

Anti-RAC1 + Cdc42 (phospho S71) antibody - ab5482 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Peptide Competition and Phosphatase Treatment: Lysates prepared from A431 cells left unstimulated (1) or stimulated with EGF (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either not treated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5482 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2, 6), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5), After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that the peptide corresponding to ab5482 blocks the antibody signal. The data also shows that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] - ab76535 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP221Y
All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535) at 1/1000 dilutionLane 1 : A431 cell lysate treated with Lambda PhosphataseLane 2 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilutionObserved band size : 24 kDa (why is the actual band size different from the predicted?)

Anti-RAC1 + RAC2 antibody - ab68828 from Abcam

Host Rabbit
Clonality Polyclonal
IHC on rat spinal cord (ventral horn) using ab68828 at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Anti-Rac1 (phospho S71) antibody - ab129743 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rac1 (phospho S71) antibody (ab129743) at 1/500 dilutionLane 1 : Extract from 293 cells, untreatedLane 2 : Extract from 293 cells treated with PMA

Anti-Rac1 antibody - ab155938 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rac1 antibody (ab155938) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : A431 cell lysateLane 4 : Jurkat cell lysateLane 5 : 3T3L1 cell lysateLysates/proteins at 50 µg per lane.SecondaryGoat anti-rabbit-HRP secondary at 1/15000 dilution

Anti-Rac1 antibody - ab97732 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rac1 antibody (ab97732) at 1/500 dilutionLane 1 : A431 cell extracts with immunizing peptideLane 2 : A431 cell extracts,

Anti-Rac1 antibody - ab97568 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rac1 antibody (ab97568) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : Raji whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Rac1 antibody - ab195606 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Rac1 antibody (ab195606) at 1/500 dilution + Human fetal brain lysate

Anti-Rac1 antibody - ab71513 from Abcam

Prices $304.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rac1 antibody (ab71513) at 1/50 dilutionLane 1 : Nontransfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the Rac1 geneLysates/proteins at 2 µg per lane.

Anti-Rac1 antibody - ab84838 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rac1 antibody (ab84838) at 1 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Rac1 antibody - ab129758 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rac1 antibody (ab129758) at 1/500 dilutionLane 1 : Untreated A431 cell extractsLane 2 : A431 cell extracts treated with EGF (200ng/ml, 5min)

Anti-Rac1 antibody - ab78139 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rac1 antibody (ab78139) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateSecondaryGoat anti-Rabbit HRP-conjugated at 1/5000 dilutionObserved band size : 22 kDa (why is the actual band size different from the predicted?)Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

Anti-Rac1 antibody [0.T.127] - ab33186 from Abcam

Prices $395.00
Sizes 125 µg
Host Mouse
Clonality Monoclonal
Clone 0.T.127
Anti-Rac1 antibody [0.T.127] (ab33186) at 1/750 dilution (for 14 hours at 4°C) + Mouse brain whole tissue lysate at 15 µgSecondaryAn HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-RAC1 Antibody (phospho-Ser71) - LS-C9854 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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