KRT20

Gene Symbol KRT20
Entrez Gene 54474
Alt Symbol CD20, CK-20, CK20, K20, KRT21
Species Human
Gene Type protein-coding
Description keratin 20, type I
Other Description cytokeratin 20|cytokeratin-20|keratin, type I cytoskeletal 20|keratin-20|protein IT
Swissprots B2R6W7 P35900
Accessions CAA51913 EAW60686 P35900 AK312744 BAG35614 BC031559 AAH31559 DA426874 DB242980 DQ890783 ABM81709 DQ896754 ABM87753 X73502 CAA51914 NM_019010 NP_061883
Function Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:10973561, ECO:0000269|PubMed:1696264}.
Tissue Specificity Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. {ECO:0000269|PubMed:10973561, ECO:0000269|PubMed:1696264}.

Keratin 20 (D9Z1Z) XP ® Rabbit mAb - 13063 from Cell Signaling Technology

Prices $109.00, $282.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9Z1Z
Western blot analysis of extracts from various cell lines using Keratin 20 (D9Z1Z) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8547 (lower).

Cytokeratin 20 (G-20) - sc-17113 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cytokeratin 20 (G-20): sc-17113. Western blot analysis of human recombinant Cytokeratin 20 fusion protein.

Cytokeratin 20 (H-70) - sc-25725 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cytokeratin 20 (H-70): sc-25725. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.

Cytokeratin 20 (E-9) - sc-271183 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cytokeratin 20 (E-9): sc-271183. Western blot analysis of human recombinant Cytokeratin 20 fusion protein.

Cytokeratin 20 (N-13) - sc-17112 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Cytokeratin 20 (N-13): sc-17112. Immunofluorescence staining of methanol-fixed HeLa cells showing cyto-skeletal localization.

Cytokeratin 20 (IT-Ks20.8) - sc-52320 from Santa Cruz Biotechnology

Prices $279.00
Sizes 500 µl supernatant
Host Mouse
Clonality Monoclonal
Cytokeratin 20 (IT-Ks20.8): sc-52320. Western blot analysis of Cytokeratin 20 expression in T84 whole cell lysate.

Cytokeratin 20 (SPM140) - sc-56522 from Santa Cruz Biotechnology

Prices $279.00
Sizes 500 µl supernatant
Host Mouse
Clonality Monoclonal
Cytokeratin 20 (SPM140): sc-56522. Western blot analysis of Cytokeratin 20 expression in T84 whole cell lysate.

pan-Cytokeratin (PK110) - sc-53403 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
pan-Cytokeratin (PK110): sc-53403. Western blot analysis of pan-Cytokeratin expression in MCF7 (A), HeLa (B), A-431 (C), Hep G2 (D) and T24 (E) whole cell lysates.

pan-Cytokeratin (PK63) - sc-53406 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
pan-Cytokeratin (PK63): sc-53406. Western blot analysis of Cytokeratin 20 expression in non-transfected 293T: sc-117752 (A), human Cytokeratin 20 transfected 293T: sc-114300 (B) and HeLa (C) whole cell lysates.

pan-Cytokeratin (K20) - sc-53402 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
pan-Cytokeratin (K20): sc-53402. Western blot analysis of pan-Cytokeratin expression in LS1034 (A) and A-431 (B) whole cell lysates.

pan-Cytokeratin (HEP 111) - sc-53401 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
pan-Cytokeratin (HEP 111): sc-53401. Western blot analysis of pan-Cytokeratin expression in Hep G2 (A) and Caco-2 (B) whole cell lysates.

Anti-Cytokeratin 20 antibody - ab118574 from Abcam

Prices $394.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab118574, at 5 µg/ml, staining Cytokeratin 20 in formalin-fixed, paraffin-embedded Human Colon tissue by Immunohistochemistry.

Anti-Cytokeratin 20 antibody - ab97511 from Abcam

Prices $385.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Cytokeratin 20 antibody (ab97511) at 1/3000 dilution + HeLa whole cell lysate at 30 µg

Anti-Cytokeratin 20 antibody - ab53120 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cytokeratin 20 antibody (ab53120) at 1/300 dilutionLane 1 : HeLa cell extractLane 2 : HeLa cell extract with immunising peptide

Anti-Cytokeratin 20 antibody - ab192682 from Abcam

Prices $370.00
Sizes 50 µl
Clonality Polyclonal

Anti-Cytokeratin 20 antibody - ab52096 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Staining of Cytokeratin 20 in human colon carcinoma by immunohistochemistry, using ab52096 (1/200). (Formalin-fixed, Paraffin embedded section).

Anti-Cytokeratin 20 antibody - ab156828 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Cytokeratin 20 antibody (ab156828) at 0.03 µg/ml + Human colon lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-Cytokeratin 20 antibody - ab156830 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Cytokeratin 20 antibody (ab156830) at 0.03 µg/ml + Human colon lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-Cytokeratin 20 antibody [2G3-1C8] - ab189165 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2G3-1C8
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human small intestine tissue sections labeling Cytokeratin 20 using ab189165 at 10 µg/ml.

Anti-Cytokeratin 20 antibody [BV12], prediluted - ab73096 from Abcam

Prices $376.00
Sizes 6 ml
Host Rabbit
Clonality Monoclonal
Clone BV12
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, staining Cytokeratin 20 with ab73096.

Anti-Cytokeratin 20 antibody [EPR1621(2)] - ab109111 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1621(2)
All lanes : Anti-Cytokeratin 20 antibody [EPR1621(2)] (ab109111) at 1/10000 dilutionLane 1 : SW480 lysateLane 2 : Mouse small intestine lysateLane 3 : Rat small intestine lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Cytokeratin 20 antibody [EPR1622Y] - ab76126 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1622Y
Overlay histogram showing LoVo cells stained with ab76126 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76126, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Cytokeratin 20 antibody [ITKS2010] (FITC) - ab189087 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone ITKS2010
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine tissue, labeling Cytokeratin 20 using ab189087 at 1/50 dilution.

Anti-Cytokeratin 20 antibody [Ks20.8] - ab854 from Abcam

Prices $385.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone Ks20.8
ICC/IF image of ab854 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab854, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-Cytokeratin 20 antibody [Ks20.8], prediluted - ab962 from Abcam

Prices $376.00
Sizes 6 ml
Host Mouse
Clonality Monoclonal
Clone Ks20.8
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