CHEK2

Gene Symbol CHEK2
Entrez Gene 11200
Alt Symbol CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
Species Human
Gene Type protein-coding
Description checkpoint kinase 2
Other Description CHK2 checkpoint homolog|cds1 homolog|checkpoint-like protein CHK2|serine/threonine-protein kinase Chk2
Swissprots Q9UGF1 Q6QA07 Q6QA08 Q6QA06 Q6QA10 Q9HCQ8 Q6QA12 B7ZBF5 Q6QA05 B7ZBF4 Q9HBS5 B7ZBF3 A8K3Y9 Q9UGF0 O96017 Q6QA03 Q6QA13 Q6QA04 Q6QA11
Accessions AAV41895 EAW59754 EAW59755 EAW59756 EAW59757 O96017 AB040105 BAB17231 AB164374 AF086904 AAC83693 AF096279 AAD11784 AF174135 AAD48504 AF217975 AAG17218 AJ131197 CAA10319 AJ783839 CAH04270 AK289360 BAF82049 AK290754 BAF83443 AW778747 AY551295 AAS58456 AY551296 AAS58457 AY551297 AAS58458 AY551298 AAS58459 AY551299 AAS58460 AY551300 AAS58461 AY551301 AAS58462 AY551302 AAS58463 AY551303 AAS58464 AY551304 AAS58465 AY551305 AAS58466 BC004207 AAH04207 BM838597 CR456418 CAG30304 CU012979 CAK54410 CU013267 CAK54709 DQ893133 ABM84059 DQ896413 ABM87412 XM_006724114 XP_006724177 XM_006724116 XP_006724179 XM_011529839 XP_011528141 XM_011529840 XP_011528142 XM_011529841 XP_011528143 XM_011529842 XP_011528144 XM_011529843 XP_011528145 XM_011529844 XP_011528146 XM_011529845 XP_011528147 XR_937805 XR_937806 XR_937807 NM_001005735 NP_001005735 NM_001257387 NP_001244316 NM_007194 NP_009125 NM_145862 NP_665861
Function Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X- R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphor
Subcellular Location Isoform 12: Nucleus.
Tissue Specificity High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
Top Pathways Cell cycle, p53 signaling pathway, HTLV-I infection

Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb (PE Conjugate) - 12812 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C13C1
Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb (PE Conjugate).

Phospho-Chk2 (Thr68) Antibody - 2661 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Cos cells, untreated or UV-treated (100 mJ/cm 2 , 1 hr recovery), using Phospho-Chk2 (Thr68) Antibody

Phospho-Chk2 (Ser516) Antibody - 2669 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, untreated or UV-treated (50 mJ/cm2, 2hrs), using Phospho-Chk2 (Ser516) Antibody (upper) or Chk2 Antibody #2662 (lower).

Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb - 2197 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C13C1
Western blot analysis of extracts from HeLa cells, untreated or UV-treated, using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb.

Phospho-Chk2 (Ser33/35) Antibody - 2665 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, untransfected (lane 1) or transfected with Wild-type Chk2 (lane 2), Chk2 (S19A) (lane 3), Chk2 (T26S28A) (lane 4), Chk2 (S33S35A) (lane 5) or Chk2 (T68A) (lane 6), using Phospho-Chk2 (Ser33/35) Antibody.

Phospho-Chk2 (Ser19) Antibody - 2666 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, untransfected (lane 1) or transfected with Wild-type Chk2 (lane 2), Chk2 (S19A) (lane 3), Chk2 (T26S28A) (lane 4), Chk2 (S33S35A) (lane 5) or Chk2 (T68A) (lane 6), using Phospho-Chk2 (Ser19) Antibody.

Chk2 (D9C6) XP ® Rabbit mAb - 6334 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9C6
Western blot analysis of extracts from various cell lines using Chk2 (D9C6) XP ® Rabbit mAb.

Chk2 Antibody - 2662 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Chk2 Antibody.

Chk2 (1C12) Mouse mAb - 3440 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1C12
Western blot analysis of extracts from various cell types using Chk2 (1C12) Mouse mAb.

Phospho-Chk2 (Thr387) Antibody - 2668 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-Chk2 (Thr68) (80F5) Rabbit mAb - 2584 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 80F5

Phospho-Chk2 (Thr432) Antibody - 2667 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or UV-treated, using Phospho-Chk2 (Thr432) Antibody.

Chk2 (A-11) - sc-17747 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD2AP siRNA (h): sc-29984. Western blot analysis of CD2AP expression in non-transfected control (A) and CD2AP siRNA transfected (B) HeLa cells. Blot probed with CD2AP (H-290): sc-17747. Lamin A/C (H-110): sc-20681 used as specificity and loading control.

Chk2 (A-12) - sc-5278 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Chk2 (A-12): sc-5278. Western blot analysis of Chk2 expression in HeLa (A) and HL-60 (B) whole cell lysates.

Chk2 (B-4) - sc-17748 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Chk2 (B-4): sc-17748. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.

Chk2 (C-18) - sc-8813 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Chk2 (C-18): sc-8813. Western blot analysis of Chk2 expression in HeLa whole cell lysate.

Chk2 (DCS-270) - sc-56296 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Chk2 (DCS-270): sc-56296. Western blot analysis of Chk2 expression in HeLa whole cell lysate.

Chk2 (DCS-273) - sc-56297 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Chk2 (DCS-273): sc-56297. Western blot analysis of Chk2 expression in HeLa whole cell lysate.

Chk2 (H-300) - sc-9064 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Chk2 (H-300): sc-9064. Western blot analysis of Chk2 expression in HL-60 whole cell lysate.

Chk2 (N-17) - sc-8812 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Chk2 (N-17): sc-8812. Western blot analysis of Chk2 expression in heat-shocked HeLa whole cell lysate.

Chk2 (19) - sc-136251 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Chk2 (19): sc-136251. Western blot analysis of Chk2 expression in HL-60 whole cell lysate.

p-Chk2 (Ser 516) - sc-101658 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Chk2 (Ser 516): sc-101658. Western blot analysis of phosphorylated Chk2 expression in untreated (A) and UV-treated (B) HeLa whole cell lysates.

Anti-Chk2 (phospho S28) antibody - ab26340 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Chk2 (phospho S28) antibody (ab26340) at 1 µg/mlLane 1 : HeLa Gamma Irradiated Whole Cell Lysate Lane 2 : HeLa Gamma Irradiated Whole Cell Lysate with Human Chk2 (phospho S28) peptide (ab28701) at 1 µg/mlLane 3 : HeLa Gamma Irradiated Whole Cell Lysate with Human Chk2 peptide (ab28702) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Chk2 (phospho S33 + S35) antibody [EPR916(2)Y] - ab68267 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR916(2)Y
All lanes : Anti-Chk2 (phospho S33 + S35) antibody [EPR916(2)Y] (ab68267) at 1/500 dilutionLane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with irradiation.Lysates/proteins at 10 µg per lane.SecondaryHRP conjugated Goat anti-rabbit at 1/2000 dilution

Anti-Chk2 (phospho S516) antibody - ab36724 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Chk2 (phospho S516) antibody (ab36724) at 1/500 dilutionLane 1 : Hela cell extract treated with UV Lane 2 : Hela cell extract untreatedLysates/proteins at 30 µg per lane.
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