PSMB8

Gene Symbol PSMB8
Entrez Gene 5696
Alt Symbol ALDD, D6S216, D6S216E, JMP, LMP7, NKJO, PSMB5i, RING10
Species Human
Gene Type protein-coding
Description proteasome (prosome, macropain) subunit, beta type, 8
Other Description large multifunctional peptidase 7|low molecular mass protein 7|low molecular weight protein 7|macropain subunit C13|multicatalytic endopeptidase complex subunit C13|protease component C13|proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)|proteasome catalytic subunit 3i|proteasome component C13|proteasome subunit Y2|proteasome subunit beta 5i|proteasome subunit beta type-8|proteasome-related gene 7|really interesting new gene 10 protein
Swissprots Q96J48 B0UZC0 Q29824 Q5QNR8 Q5JNW6 P28062
Accessions AAA80234 AAA80235 AHW47925 AHW47942 AHW47959 AHW47976 CAA47026 CAA60786 CAA60787 CAA78705 CAA78706 EAX03644 EAX03645 P28062 AA807046 AK300962 BAH13383 AW242683 BC001114 AAH01114 BG547344 BG760674 BM547457 BM781620 CR541661 CAG46462 U17496 AAA56777 U17497 AAA56778 X62598 CAA44482 NM_004159 NP_004150 NM_148919 NP_683720
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes. {ECO:0000269|PubMed:16423992, ECO:0000269|PubMed:19443843, ECO:0000269|PubMed:21881205, ECO:0000269|PubMed:8163024}.
Subcellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809}. Nucleus {ECO:0000250}.
Top Pathways Proteasome

PSMB8/LMP7 (1A5) Mouse mAb - 13726 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1A5
Western blot analysis of extracts from various cell lines using PSMB8/LMP7 (1A5) Mouse mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). (The T2 (13) and RKO (8) cell lines are both deficient in PSMB8/LMP8 protein expression).

PSMB8/LMP7 (D1K7X) Rabbit mAb - 13635 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1K7X
Western blot analysis of extracts from various cell lines using PSMB8/LMP7 (D1K7X) Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (The T2 cell line contains a homozygous deletion of PSMB8/LMP7 (13)).

LMP7 (A-12) - sc-365699 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LMP7 (A-12): sc-365699. Western blot analysis of LMP7 expression in CCRF-CEM (A) and HuT 78 (B) whole cell lysates.

LMP7 (D-12) - sc-365698 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LMP7 (D-12): sc-365698. Western blot analysis of LMP7 expression in CCRF-CEM (A) and HuT 78 (B) whole cell lysates.

LMP7 (H-40) - sc-134503 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LMP7 (H-40): sc-134503. Western blot analysis of LMP7 expression in CCRF-CEM (A) and HuT 78 (B) whole cell lysates.

LMP7 (S-19) - sc-31313 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LMP7 (S-19): sc-31313. Immunoperoxidase staining of formalin fixed, paraffin-embedded human recturm tissue showing cytoplasmic and membrane staining of glandular cells.

LMP7 (K-15) - sc-17073 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LMP7 (K-15): sc-17073. Western blot analysis of LMP7 expression in CCRF-CEM (A), HuT 78 (B) and MOLT-4 (C) whole cell lysates.

LMP7 (D-2) - sc-374089 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LMP7 (D-2): sc-374089. Western blot analysis of LMP7 expression in CCRF-CEM (A) and HuT 78 (B) whole cell lysates.

LMP7 (56-T) - sc-100284 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
LMP7 (56-T): sc-100284. Immunofluorescence staining of paraformaldehyde-fixed HeLa cells showing nuclear localization.

LMP7A (E-20) - sc-17070 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LMP7A (E-20): sc-17070. Western blot analysis of LMP7A expression in CCRF-CEM (A), HuT 78 (B), MOLT-4 (C) and HeLa (D) whole cell lysates.

LMP7A (L-20) - sc-17069 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LMP7A (L-20): sc17069. Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.

Anti-Proteasome 20S LMP7 antibody - ab58094 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Proteasome 20S LMP7 antibody (ab58094) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.

Anti-Proteasome 20S LMP7 antibody - ab88820 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Proteasome 20S LMP7 antibody (ab88820) at 1/500 dilution + Human placenta lysate at 50 µg

Anti-Proteasome 20S LMP7 antibody [1A5] - ab119959 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1A5
Overlay histogram showing MCF7 cells stained with ab119959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119959, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Proteasome 20S LMP7 antibody [EPR14482(B)] - ab180606 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14482(B)
Western blot analysis of Raji lysate immunoprecipitated with ab180606 at 1/40 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG used at 1/1500 dilution.

Anti-PSMB8 / LMP7 Antibody - LS-C86224 from LifeSpan Bioscience

Prices $800.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSMB8 / LMP7 Antibody (aa259-274) - LS-C89601 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSMB8 / LMP7 Antibody (aa7-36, APC) - LS-C267293 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB8 / LMP7 Antibody (aa7-36, AP) - LS-C267292 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB8 / LMP7 Antibody (aa7-36, Biotin) - LS-C267294 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB8 / LMP7 Antibody (N-Terminus) - LS-C201504 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human HeLa. PSMB8 antibody dilution 1.0 ug/ml.

Anti-PSMB8 / LMP7 Antibody (aa7-36, FITC) - LS-C267295 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB8 / LMP7 Antibody (aa7-36, PE) - LS-C267297 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB8 / LMP7 Antibody (aa7-36, Azide-free, HRP) - LS-C267298 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB8 / LMP7 Antibody (aa7-36) - LS-C162235 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PSMB8 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The PSMB8 antibody detected the PSMB8 protein (arrow).
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