ARAF

Gene Symbol ARAF
Entrez Gene 369
Alt Symbol A-RAF, ARAF1, PKS2, RAFA1
Species Human
Gene Type protein-coding
Description A-Raf proto-oncogene, serine/threonine kinase
Other Description A-Raf proto-oncogene serine/threonine-protein kinase|Oncogene ARAF1|Ras-binding protein DA-Raf|proto-oncogene A-Raf-1|proto-oncogene Pks|serine/threonine-protein kinase A-Raf|v-raf murine sarcoma 3611 viral oncogene homolog 1
Swissprots Q5H9B2 P07557 Q5H9B3 P10398
Accessions AAA65219 AAB03517 EAW59310 EAW59311 EAW59312 P10398 AB158254 BAE66645 AB208831 BAD92068 AF052136 AK130043 AK297453 BAG59877 AK300975 BAG62597 AK307747 BC002466 AAH02466 BC007514 AAH07514 BT019864 AAV38667 CA424869 DA264472 DC362427 DQ892656 ABM83582 DQ895896 ABM86822 M13829 AAB08754 X04790 CAA28476 XM_006724529 XP_006724592 XM_011543906 XP_011542208 XM_011543907 XP_011542209 XM_011543908 XP_011542210 XM_011543909 XP_011542211 NM_001256196 NP_001243125 NM_001256197 NP_001243126 NM_001654 NP_001645
Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. {ECO:0000269|PubMed:22609986}.
Tissue Specificity Predominantly in urogenital tissues.
Top Pathways Proteoglycans in cancer, Vascular smooth muscle contraction, Hepatitis C, Alcoholism, Natural killer cell mediated cytotoxicity

Phospho-A-Raf (Ser299) Antibody - 4431 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Raji and HeLa cells, untreated or TPA-treated (30 minutes), using Phospho-A-Raf (Ser299) Antibody (upper) or A-Raf Antibody, #4432 (lower).

A-Raf Antibody - 4432 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT-29, NIH-3T3 and C6 cell lysates, using A-Raf Antibody.

A-Raf (A-5) - sc-166771 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
A-Raf (A-5): sc-166771. Western blot analysis of A-Raf expression in K-562 (A) and Hela (B) whole cell lysates.

A-Raf (C-20) - sc-408 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
A-Raf (C-20): sc-408. Western blot analysis of A-Raf expression in K-562 whole cell lysate.

A-Raf (H-70) - sc-28772 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
A-Raf (H-70): sc-28772. Western blot analysis of A-Raf expression in non-transfected: sc-117752 (A) and human A-Raf transfected: sc-159320 (B) 293T whole cell lysates.

A-Raf (T-15) - sc-30702 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
A-Raf (T-15): sc-30702. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

A-Raf (C-18) - sc-30704 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

A-Raf (1) - sc-135820 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
A-Raf (1): sc-135820. Western blot analysis of A-Raf expression in SK-N whole cell lysate.

p-A-Raf (Ser 299) - sc-109899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-A-Raf (Ser 299): sc-109899. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

Anti-A RAF (phospho S299) antibody - ab112053 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab112053 at 1/50 dilution staining A RAF in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phospho peptide.

Anti-A RAF (phospho Y301 + Y302) antibody - ab59439 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-A RAF (phospho Y301 + Y302) antibody (ab59439) at 1/500 dilutionLane 1 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins)Lane 2 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins) with phosphopeptide

Anti-A RAF antibody - ab96712 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-A RAF antibody (ab96712) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-A RAF antibody - ab170253 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed paraffin embedded Human breast carcinoma tissue labeling A RAF with ab170253 at 1/100 dilution.

Anti-A RAF antibody - ab59343 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-A RAF antibody (ab59343) at 1/500 dilutionLane 1 : Extracts from HeLa cells treated with PMA (125ng/ml, 30mins) Lane 2 : Extracts from HeLa cells treated with PMA (125ng/ml, 30mins), plus immunising peptideObserved band size : 68 kDa (why is the actual band size different from the predicted?)

Anti-A RAF antibody - ab136070 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-A RAF antibody (ab136070) at 1/500 dilution + HeLa cell lysate at 30 µg

Anti-A RAF antibody - ab54827 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-A RAF antibody - ab113271 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of A RAF by Western Blot of Immunprecipitate. anti-A RAF at 1µg/ml staining A RAF in HeLa whole cell lysate immunoprecipitated using ab113271 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-A RAF antibody [EPR16208] - ab200653 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16208
All lanes : Anti-A RAF antibody [EPR16208] (ab200653) at 1/10000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-ARAF / ARAF1 / A-RAF Antibody (aa151-251) - LS-C131791 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-ARAF / ARAF1 / A-RAF Antibody (aa248-277, Biotin) - LS-C251600 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARAF / ARAF1 / A-RAF Antibody (aa248-277, AP) - LS-C251598 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARAF / ARAF1 / A-RAF Antibody (aa248-277, APC) - LS-C251599 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARAF / ARAF1 / A-RAF Antibody (aa254-303) - LS-C81879 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-ARAF / ARAF1 / A-RAF Antibody (aa256-286) - LS-C97185 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-ARAF / ARAF1 / A-RAF Antibody (aa248-277, Azide-free, HRP) - LS-C251602 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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