NPM1

Gene Symbol NPM1
Entrez Gene 4869
Alt Symbol B23, NPM
Species Human
Gene Type protein-coding
Description nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Other Description nucleolar protein NO38|nucleophosmin|nucleophosmin/nucleoplasmin family, member 1
Swissprots A8K3N7 P06748 Q96EA5 Q8WTW5 B5BU00 Q5EU97 Q5EU98 Q5EU96 Q5EU99 P08693 Q12826 Q9UDJ7 Q96AT6 Q13440 Q5EU95 Q9BYG9 Q5EU94 Q14115 Q96DC4 D3DQL6 Q13441 Q6V962
Accessions AAB94739 ACA50454 EAW61443 EAW61444 EAW61445 EAW61446 EAW61447 EAW61448 EAW61449 P06748 AB042278 BAB40600 AB451236 BAG70050 AB451361 BAG70175 AI687394 AK000472 BAA91188 AK290652 BAF83341 AY347529 AAQ24860 AY740634 AAW67752 AY740635 AAW67753 AY740636 AAW67754 AY740637 AAW67755 AY740638 AAW67756 AY740639 AAW67757 AY740640 AAW67758 BC002398 AAH02398 BC003670 AAH03670 BC008495 AAH08495 BC009623 AAH09623 BC012566 AAH12566 BC014349 AAH14349 BC016716 AAH16716 BC016768 AAH16768 BC016824 AAH16824 BC020467 BC021668 AAH21668 BC021983 AAH21983 BC024716 BC050628 AAH50628 BC107754 AAI07755 BP291033 BT007011 AAP35657 BU661768 D28343 BAA05709 DQ303464 ABC40399 DQ892032 ABM82958 DQ895223 ABM86149 EF429251 ABP97432 J02590 AAA36473 M23613 AAA36380 M26697 AAA36385 M28699 AAA58386 M31004 AAA36474 X16934 CAA34809 XM_005265920 XP_005265977 XM_011534564 XP_011532866 NM_001037738 NP_001032827 NM_002520 NP_002511 NM_199185 NP_954654
Function Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylati
Subcellular Location Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.

Phospho-NPM (Ser4) (D19C1) XP ® Rabbit mAb - 3520 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D19C1
Western blot analysis of extracts of HeLa cells, either untreated or nocodazole-treated, using Phospho-NPM (Ser4) (D19C1) XP ® Rabbit mAb (upper). Lower panel shows total NPM using NPM Antibody #3542.

Phospho-NPM (Thr199) Antibody - 3541 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using Phospho-NPM (Thr199) Antibody (upper) or NPM Antibody #3542 (lower).

Phospho-NPM (Thr95) Antibody - 3517 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of HeLa cells, untreated or nocodazole-treated, using Phospho-NPM (Thr95) Antibody (upper) or NPM Antibody #3542 (lower).

NPM Antibody - 3542 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SW-13, NIH/3T3, Jurkat and PC12 cells, using NPM Antibody.

B23 (0412) - sc-47725 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B23 (0412): sc-47725. Western blot analysis of B23 expression in non-transfected: sc-110760 (A) and human B23 transfected: sc-111207 (B) 293 whole cell lysates.

B23 (E-3) - sc-271737 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B23 (E-3): sc-271737. Western blot analysis of B23 expression in 293T (A), HeLa (B) and HEL 92.1.7 (C) whole cell lysates.

B23 (FC-8791) - sc-32256 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B23 (FC-8791): sc-32256. Western blot analysis of B23 expression in non-transfected: sc-110760 (A) and human B23 transfected: sc-111207 (B) 293 whole cell lysates.

B23 (H-106) - sc-5564 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
B23 (H-106): sc-5564. Western blot analysis of B23 expression in HEL 92.1.7 (A), LNCaP (B) and HeLa (C) whole cell lysates.

B23 (FC82291) - sc-56622 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B23 (FC82291): sc-56622. Western blot analysis of B23 expression in non-transfected: sc-110760 (A) and human B23 transfected: sc-111207 (B) 293 whole cell lysates.

B23 (NA24) - sc-53175 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B23 (NA24): sc-53175. Western blot analysis of B23 expression in non-transfected: sc-110760 (A) and human B23 transfected: sc-111207 (B) 293 whole cell lysates.

B23 (7H10B9) - sc-65943 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
B23 (7H10B9): sc-65943. Western blot analysis of B23 expression in non-transfected: sc-110760 (A) and human B23 transfected: sc-111207 (B) 293 whole cell lysates.

B23 (3F291) - sc-70392 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
B23 (3F291): sc-70392. Western blot analysis of B23 expression in non-transfected: sc-110760 (A) and human B23 transfected: sc-111207 (B) 293 whole cell lysates.

Anti-Mutant Nucleophosmin (AML marker) antibody - Carboxyterminal end - ab65816 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Mutant Nucleophosmin localization by immunofluorescence in OCI-AML3 cells using 1/50 ab65816.

Anti-Nucleophosmin (phospho S125) antibody [EPR1856] - ab109546 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1856
Overlay histogram showing HeLa cells stained with ab109546 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109546, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Nucleophosmin (phospho T199) antibody - ab59353 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59353, at a 1/50 dilution, staining human Nucleophosmin in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and in the presence of the immunizing peptide (right image).

Anti-Nucleophosmin (phospho T199) antibody - ab118641 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab118641, at a 1/50 dilution, staining Nucleophosmin (phospho T199) in formalin fixed, paraffin embedded Human thymus tissue by Immunohistochemistry. Detection utilized biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Nucleophosmin (phospho T199) antibody - ab192181 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nucleophosmin (phospho T199) antibody (ab192181) at 1/500 dilutionLane 1 : Jurkat whole cell lysate with the antigen-specific peptideLane 2 : Jurkat whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] - ab81551 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EP1857Y
All lanes : Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab81551) at 1/2500 dilutionLane 1 : lysate from untreated HeLa cells Lane 2 : lysate from HeLa cells treated with CALysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution

Anti-Nucleophosmin (phospho T234 + T237) antibody - ab118637 from Abcam

Prices $407.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab118637 at 1/50 dilution staining Nucleophosmin (phospho T234 + T237) in Formalin-Fixed, Paraffin-Embedded Human Skin tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Nucleophosmin (phospho T234) antibody - ab138673 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Nucleophosmin (phospho T234) antibody (ab138673) at 1/500 dilution + HeLa cell lysate (from cells treated with 1ug/ml nocodazole for 18h) at 30 µg

Anti-Nucleophosmin (phospho T95) antibody [EPNCIR117] - ab133453 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR117
All lanes : Anti-Nucleophosmin (phospho T95) antibody [EPNCIR117] (ab133453) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Anti-Nucleophosmin antibody - ab15440 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab15440 detecting Nucleophosmin in interphase mouse primary embryonic fibroblasts. Cells were also counterstained with anti gamma tubulin antibody and DAPI in order to highlight the centrossomes and the nucleus (respectively). Secondary antibodies: goat anti-rabbit IgG conjugated to Alexa 488 (for ab15440) and goat anti-mouse IgG conjugated to Alexa 594 (for gamma-tubulin antibody).

Anti-Nucleophosmin antibody - ab37659 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nucleophosmin antibody (ab37659) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Nucleophosmin antibody - ab24412 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Nucleophosmin antibody (ab24412) at 1 µg/ml + HepG2 cell lysate (10ug).SecondaryHRP conjugated Anti rabbit IgG at 1/50,000 - 1/100,000.

Anti-Nucleophosmin antibody - ab55708 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Nucleophosmin antibody (ab55708) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma tissue.
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