| Gene Symbol | SMAD3 |
|---|---|
| Entrez Gene | 4088 |
| Alt Symbol | HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3 |
| Species | Human |
| Gene Type | protein-coding |
| Description | SMAD family member 3 |
| Other Description | MAD homolog 3|MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|hMAD-3|hSMAD3|mad homolog JV15-2|mad protein homolog|mad3|mothers against DPP homolog 3|mothers against decapentaplegic homolog 3 |
| Swissprots | B7Z9Q2 B7Z4Z5 P84022 A8K4B6 B7Z6M9 F5H383 |
| Accessions | AAL68976 BAA22032 EAW77788 EAW77789 EAW77790 EAW77791 P84022 AA493306 AB209616 BAD92853 AF151027 AAF36113 AI762628 AI991957 AK026690 AK290881 BAF83570 AK298139 BAH12731 AK300614 BAH13315 AK301348 BAH13461 AK313812 BAG36548 AK316017 BAH14388 AL110265 BC000414 BC007496 BC050743 AAH50743 BE905995 BF058230 BG251847 BM551682 BM722372 BQ917888 BX506233 CR457386 CAG33667 CR749287 CAH18142 DA453132 DA755047 DA977882 EU016552 EU016553 EU446487 ABZ92016 U68019 AAB80960 U76622 AAB18967 XM_011521559 XP_011519861 XM_011521560 XP_011519862 NM_001145102 NP_001138574 NM_001145103 NP_001138575 NM_001145104 NP_001138576 NM_005902 NP_005893 |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. {ECO:0000269|PubMed:10995748, ECO:0000269|PubMed:15241418, ECO:00 |
| Subcellular Location | Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta. |
| Top Pathways | Cell cycle, Signaling pathways regulating pluripotency of stem cells, Inflammatory bowel disease (IBD), TGF-beta signaling pathway, Adherens junction |
Smad3 CRISPR/Cas9 KO Plasmid (h) - sc-400069 from Santa Cruz Biotechnology
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Smad3 HDR Plasmid (h) - sc-400069-HDR from Santa Cruz Biotechnology
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Smad3 Double Nickase Plasmid (h) - sc-400069-NIC from Santa Cruz Biotechnology
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Smad3 Double Nickase Plasmid (h2) - sc-400069-NIC-2 from Santa Cruz Biotechnology
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Smad3 CRISPR Activation Plasmid (h) - sc-400069-ACT from Santa Cruz Biotechnology
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Smad3 CRISPR Activation Plasmid (h2) - sc-400069-ACT-2 from Santa Cruz Biotechnology
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Smad3 Lentiviral Activation Particles (h) - sc-400069-LAC from Santa Cruz Biotechnology
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Smad3 Lentiviral Activation Particles (h2) - sc-400069-LAC-2 from Santa Cruz Biotechnology
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Smad3 siRNA (h) - sc-38376 from Santa Cruz Biotechnology
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Smad3 shRNA Plasmid (h) - sc-38376-SH from Santa Cruz Biotechnology
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Smad3 shRNA (h) Lentiviral Particles - sc-38376-V from Santa Cruz Biotechnology
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Smad3 RNAi - H00004088-R01 from Novus Biologicals
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MISSION® esiRNA - EHU037241 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN19751 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND19751 from Sigma-Aldrich
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SMAD3 siRNA (Human) - CRH2771 from Cohesion Biosciences
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SMAD3 - MBS8235225 from MyBioSource
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shRNA set against Human SMAD3 (NM_005902.3) - SHH412972 from Creative biogene
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shRNA set against Human SMAD3(NM_005902.3) - SHL015174 from Creative biogene
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Smad3 CRISPR/Cas9 KO Plasmid (h2) - sc-400069-KO-2 from Santa Cruz Biotechnology
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Smad3 HDR Plasmid (h2) - sc-400069-HDR-2 from Santa Cruz Biotechnology
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SMAD3 shRNA Plasmid (Human) - CHH2771 from Cohesion Biosciences
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SMAD3 miRNA 3'UTR clone - MiUTR1H-09636 from Creative biogene
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