ARF1

Gene Symbol ARF1
Entrez Gene 338058
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description ADP-ribosylation factor 1
Other Description -
Swissprots A5D7F1 Q3SZG1 P84080
Accessions DAA28323 P84080 BC102874 AAI02875 BC140532 AAI40533 J03275 AAA30361 XM_005208399 XP_005208456 XM_005208400 XP_005208457 NM_176653 NP_788826
Function GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Subcellular Location Golgi apparatus. Cytoplasm, perinuclear region {ECO:0000250}. Cell junction, synapse, synaptosome {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}.
Top Pathways Endocytosis, Legionellosis

Anti-ADP Ribosylation Factor antibody [1D9] - ab2806 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1D9
Immunocytochemistry/Immunofluorescence analysis of ADP Ribosylation Factor shows staining in HeLa cells. ADP Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2806 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Anti-ARF1 antibody - ab58578 from Abcam

Prices $392.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-ARF1 antibody (ab58578) at 1 µg/ml + HepG2 cell lysate at 35 µgdeveloped using the ECL technique

Anti-ARF1 antibody - ab183576 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ARF1 antibody (ab183576) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : U2 OS cell lysateLane 3 : 3T3 cell lysateLane 4 : NRK cell lysateLane 5 : MDCK cell lysateLysates/proteins at 25 µg per lane.Secondarygoat anti-rabbit IgG-HRP at 1/1500 dilutiondeveloped using the ECL technique

Anti-ARF1 antibody - ab151194 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-ARF1 antibody [3F1] - C-terminal (FITC) - ab187824 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3F1

Anti-ARF1 antibody [ARFS 3F1] - ab90356 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone ARFS 3F1
All lanes : Anti-ARF1 antibody [ARFS 3F1] (ab90356) at 1/250 dilutionLane 1 : Rat brain tissue extractLane 2 : Mouse brain tissue extractLane 3 : MDCKdeveloped using the ECL technique

Rabbit Anti-Human ARF1 Polyclonal antibody - DPABH-00140 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal
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