DNMT1

Gene Symbol DNMT1
Entrez Gene 1786
Alt Symbol ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT
Species Human
Gene Type protein-coding
Description DNA (cytosine-5-)-methyltransferase 1
Other Description CXXC-type zinc finger protein 9|DNA (cytosine-5)-methyltransferase 1|DNA MTase HsaI|DNA methyltransferase HsaI|m.HsaI
Swissprots P26358 A0AV63 Q9ULA2 Q9UHG5 B7ZLW6 Q9UMZ6
Accessions AAD54507 EAW84079 EAW84080 P26358 AB209413 BAD92650 AF180682 AAF23609 AF290894 AK122759 BAG53712 AY927518 BC092517 AAH92517 BC126227 AAI26228 BC144093 AAI44094 DA653750 X63692 CAA45219 XM_011527772 XP_011526074 XM_011527773 XP_011526075 XM_011527774 XP_011526076 NM_001130823 NP_001124295 NM_001379 NP_001370
Function Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. {ECO:0000269|PubMed:16357870, ECO:0000269|PubMed:18413740, ECO:0000269|PubMed:18754681}.
Subcellular Location Nucleus {ECO:0000269|PubMed:12145218}.
Tissue Specificity Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. {ECO:0000269|PubMed:10325416}.
Top Pathways Cysteine and methionine metabolism, MicroRNAs in cancer

DNMT1 (D63A6) XP ® Rabbit mAb - 5032 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D63A6
Western blot analysis of extracts from 293 and F9 cells using DNMT1 (D63A6) XP ® Rabbit mAb.

DNMT1 (D59A4) Rabbit mAb - 5119 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D59A4
Western blot analysis of extracts from various cell lines using DNMT1 (D59A4) Rabbit mAb.

Dnmt1 (H-12) - sc-271729 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dnmt1 (H-12): sc-271729. Western blot analysis of Dnmt1 expression in Saos-2 (A) and HeLa (B) whole cell lysates.

Dnmt1 (H-300) - sc-20701 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Dnmt1 (H-300): sc-20701. Western blot analysis of Dnmt1 isoform expression in HeLa (A) and A549 (B) whole cell lysates.

Dnmt1 (K-18) - sc-10221 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dnmt1 (K-18): sc-10221. Western blot analysis of Dnmt1 expression in F9 whole cell lysate.

Dnmt1 (C-17) - sc-10222 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dnmt1 (C-17): sc-10222. Western blot analysis of Dnmt1 expression in HeLa (A) and A549 (B) whole cell lysates.

Dnmt1 (N-16) - sc-10219 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dnmt1 (N-16): sc-10219. Western blot analysis of Dnmt1 expression in HeLa whole cell lysate.

Dnmt1 (18) - sc-135887 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Dnmt1 (18): sc-135887. Western blot analysis of Dnmt1 expression in Jurkat whole cell lysate.

p-Dnmt1 (Ser 714) - sc-130595 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

p-Dnmt1 (Tyr 399) - sc-130596 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

p-Dnmt1 (Tyr 969) - sc-130597 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-Dnmt1 antibody - ab19905 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Dnmt1 antibody (ab19905) at 1 µg/mlLane 1 : HeLa nuclear lysateLane 2 : HeLa nuclear lysate with Human Dnmt1 peptide (ab21999) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Dnmt1 antibody - ab87654 from Abcam

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.

Anti-Dnmt1 antibody - ab2849 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Dnmt1 antibody - ab11892 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Dnmt1 antibody - ab45281 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Dnmt1 antibody [2B5] - ab54759 from Abcam

Prices $390.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2B5
Dnmt1 antibody (ab54759) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human placenta.

Anti-Dnmt1 antibody [60B12201] - ChIP Grade - ab176145 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 60B12201
Anti-Dnmt1 antibody [60B12201] - ChIP Grade (ab176145) at 2 µg/ml + Mouse embryonic Cell lysate at 10 µg

Anti-Dnmt1 antibody [7C11A2] - C-terminal - ab181462 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7C11A2
Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Dnmt1 with ab181462 at 1/200 dilution.

Anti-Dnmt1 antibody [EPR3521(2)] - ab134148 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3521(2)
All lanes : Anti-Dnmt1 antibody [EPR3521(2)] (ab134148) at 1/1000 dilutionLane 1 : Jurkat cell lysatesLane 2 : 293T cell lysatesLane 3 : HeLa cell lysatesLane 4 : HuT-78 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Dnmt1 antibody [EPR3521(2)] (Alexa Fluor® 488) - ab197272 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3521(2)
Overlay histogram showing HeLa cells stained with ab197272 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197272, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Dnmt1 antibody [EPR3521(2)] (HRP) - ab196927 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3521(2)
All lanes : Anti-Dnmt1 antibody [EPR3521(2)] (HRP) (ab196927) at 1/3000 dilutionLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Dnmt1 antibody [EPR3522] - ab92314 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3522
Overlay histogram showing HeLa cells stained with ab92314 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92314, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Dnmt1 antibody - ChIP Grade - ab87656 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Dnmt1 antibody - ChIP Grade (ab87656) at 1 µg/mlLane 1 : lysate from Mouse erythrolekemia cells MEL11A2 Lane 2 : lysate from untransfected Mouse myoblast cells C2C12 Lane 3 : lysate from C2C12 cells transfected with Dnmt1 overexpressing plasmidLane 4 : lysate from C2C12 cells transfected with negative control plasmid

Anti-Dnmt1 antibody - N-terminal - ab152153 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Dnmt1 antibody - N-terminal (ab152153) at 1/1000 dilution + Raji whole cell lysate at 30 µg
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