PDPK1

Gene Symbol PDPK1
Entrez Gene 5170
Alt Symbol PDK1, PDPK2, PDPK2P, PRO0461
Species Human
Gene Type protein-coding
Description 3-phosphoinositide dependent protein kinase 1
Other Description 3-phosphoinositide dependent protein kinase-1|3-phosphoinositide-dependent protein kinase 1|3-phosphoinositide-dependent protein kinase 2 pseudogene|PkB kinase like gene 1
Swissprots Q6FI20 Q8IV52 Q9BRD5 Q59EH6 H0Y4Z0 O15530
Accessions AAC33797 CAC10873 EAW85495 O15530 Q6A1A2 AB209835 BAD93072 AF017995 AAC51825 AF111845 AAF16685 AJ785968 CAH05056 AK095507 AK131060 AK222581 BAD96301 AK293900 BAG57287 AL157446 BC000789 BC006339 AAH06339 BC012103 AAH12103 BC012540 BC033494 AAH33494 BC040324 BI830733 CR536517 CAG38755 DQ892297 ABM83223 DQ895496 ABM86422 EU832784 ACE87795 GQ129441 ACT64575 Y15056 CAA75341 XM_005255356 XP_005255413 XM_011522521 XP_011520823 XM_011522522 XP_011520824 XM_011522523 XP_011520825 XM_011522524 XP_011520826 NM_001261816 NP_001248745 NM_002613 NP_002604 NM_031268 NP_112558
Function Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translo
Subcellular Location Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion. Note=Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non- neuronal cells it is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resulting in its translocation to other cellular compartments. The tyrosine-phosphorylated form colocalizes with PTK2B in focal adhesions after angiotensin II stimulation.
Tissue Specificity Appears to be expressed ubiquitously. The Tyr- 9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast. {ECO:0000269|PubMed:18024423}.
Top Pathways Proteoglycans in cancer, AMPK signaling pathway, PI3K-Akt signaling pathway, Choline metabolism in cancer, Sphingolipid signaling pathway

Phospho-PDK1 (Ser241) Antibody - 3061 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells (starved for 16 hours) treated with PDGF (50 ng/ml), using Phospho-PDK1 (Ser241) Antibody (upper) or control PDK1 antibody (lower). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer.

Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb - 3438 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C49H2
Western blot analysis of extracts from PC3 cells, HCT116 wild-type and HCT116 PDK1 -/- cells using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT116 wild-type and HCT116 PDK1 -/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

PDK1 (D4Q4D) Rabbit mAb - 13037 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4Q4D
Western blot analysis of extracts from HCT 116 wild-type and HCT 116 PDK1-/- cells using PDK1 (D4Q4D) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT 116 wild-type and HCT 116 PDK1-/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD).

PDK1 (D37A7) Rabbit mAb - 5662 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D37A7
Western blot analysis of extracts from various cell lines using PDK1 (D37A7) Rabbit mAb.

PDK1 Antibody - 3062 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SW-13, NIH/3T3, Jurkat and PC12 cells, using PDK1 Antibody.

Phospho-PDK1 (Tyr373/376) Antibody - 3065 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

PKB Kinase (D-5) - sc-376586 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKB Kinase (D-5): sc-376586. Western blot analysis of PKB Kinase expression in MCF7 whole cell lysate.

PKB Kinase (C-19) - sc-7686 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PKB Kinase (C-19): sc-7686. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic and membrane staining.

PKB Kinase (A-10) - sc-17766 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKB Kinase (A-10): sc-17766. Western blot analysis of PKB Kinase expression in MCF7 (A), U-937 (B), A-431 (C), CCD-1064Sk (D) and KNRK (E) whole cell lysates.

PKB Kinase (E-3) - sc-17765 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKB Kinase (E-3): sc-17765. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and membrane staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

PKB Kinase (H-328) - sc-9118 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKB Kinase (H-328): sc-9118. Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic and membrane staining.

PKB Kinase (5) - sc-136243 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
PKB Kinase (5): sc-136243. Western blot analysis of PKB Kinase expression in SW13 whole cell lysate.

p-PKB Kinase (Ser 241) - sc-32960 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-PKB Kinase (Ser 241): sc-32960. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing membrane, cytoplasmic and nuclear staining of decidual cells.

p-PKB Kinase (Ser 241) - sc-101775 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-PKB Kinase (Ser 241): sc-101775. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.

Anti-PDPK1 (phospho S241) antibody - ab32800 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDPK1 (phospho S241) antibody (ab32800) at 1/1000 dilutionLane 1 : MDA MB 435 cell extract treated with EGF.Lane 2 : COS7 cell extract treated with TNF.

Anti-PDPK1 (phospho S241) antibody - ab131098 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDPK1 (phospho S241) antibody (ab131098) at 1/500 dilutionLane 1 : 293 cell extract, untreatedLane 2 : 293 cell extract treated with EGFLane 3 : PC12 cell extract, untreatedLane 4 : PC12 cell extract treated with EGF

Anti-PDPK1 (phospho S241) antibody [EPR336(2)] - ab109460 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR336(2)
All lanes : Anti-PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460) at 1/1000 dilutionLane 1 : 293T cell lysate Lane 2 : 293T cell lysate treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.SecondaryHRP-labeled goat anti-rabbit at 1/2000 dilution

Anti-PDPK1 (phospho S410) antibody [EPR6157] - ab133461 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6157
All lanes : Anti-PDPK1 (phospho S410) antibody [EPR6157] (ab133461) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : 293T cell lysate treated with Okadaic Acid and Calyculin A.Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution

Anti-PDPK1 (phospho Y9) antibody - ab111863 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111863 at 1/50 dilution staining PDPK1 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-PDPK1 (phospho Y9) antibody - ab45375 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PDPK1 antibody - ab186870 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186870. Blue DAPI for nuclear staining.

Anti-PDPK1 antibody - ab168406 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PDPK1 antibody (ab168406) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

Anti-PDPK1 antibody - ab19034 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PDPK1 antibody - ab71421 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDPK1 antibody (ab71421) at 1/50 dilutionLane 1 : Nontransfected 293 cell lysatesLane 2 : 293 cell lysates transiently transfected with the PDPK1 geneLysates/proteins at 2 µg per lane.

Anti-PDPK1 antibody - ab45374 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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