LGALS3

Gene Symbol LGALS3
Entrez Gene 3958
Alt Symbol CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2
Species Human
Gene Type protein-coding
Description lectin, galactoside-binding, soluble, 3
Other Description 35 kDa lectin|IgE-binding protein|MAC-2 antigen|carbohydrate-binding protein 35|galactose-specific lectin 3|galectin-3|laminin-binding protein|lectin L-29
Swissprots B2RC38 P17931 Q96J47 Q6IBA7 Q16005
Accessions AAB86584 BAC45249 CBX53892 EAW80658 EAW80660 P17931 AB006780 BAA22164 AB209391 BAD92628 AF266280 AAG44701 AK314929 BAG37435 BC001120 AAH01120 BC053667 AAH53667 BC068068 AAH68068 BC128253 BC128403 BG332134 BQ645629 BU681070 BX161455 CAD61918 BX641090 CAE46042 CD518618 CR456897 CAG33178 CR542097 CAG46894 DQ892594 ABM83520 EU176706 ABW03507 M35368 AAA88086 M36682 AAA36163 M57710 AAA35607 M64303 S59012 AAB26229 XM_011536759 XP_011535061 NM_001177388 NP_001170859 NM_002306 NP_002297 NR_003225
Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. {ECO:0000250, ECO:0000269|PubMed:15181153, ECO:0000269|PubMed:19594635, ECO:0000269|PubMed:19616076}.
Subcellular Location Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface.
Tissue Specificity A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. {ECO:0000269|PubMed:19497882}.

Galectin-3/LGALS3 Antibody - 12733 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Galectin-3/LGALS3 Antibody.

galectin-3 (H-5) - sc-374253 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
galectin-3 (H-5): sc-374253. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

galectin-3 (H-160) - sc-20157 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
galectin-3 (H-160): sc-20157. Immunofluorescence staining of methanol-fixed RAW 264.7 cells (A) and immunoperoxidase staining of formalin fixed, paraffin-embedded mouse colon tissue (B) showing nuclear and cytoplasmic localization.

galectin-3 (B2C10) - sc-32790 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
galectin-3 (B2C10): sc-32790. Western blot analysis of galectin-3 expression in HeLa (A) and SW480 (B) nuclear extracts and A-431 (C) and T84 (D) whole cell lysates.

galectin-3 (D-20) - sc-19283 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
galectin-3 (D-20): sc-19283. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

galectin-3 (N-20) - sc-19280 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
galectin-3 (N-20): sc-19280. Western blot analysis of galectin-3 expression in HeLa (A), MCF7 (B), SW480 (C) and WEHI-231 (D) nuclear extracts.

galectin-3 (B-2) - sc-25279 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
galectin-3 (B-2): sc-25279. Western blot analysis of galectin-3 expression in A-431 nuclear extract (A) and Hs 294T (B), T84 (C) and SW480 (D) whole cell lysates.

galectin-3 (M3/38) - sc-23938 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
galectin-3 (M3/38): sc-23938. Western blot analysis of galectin-3 expressin in HeLa (A), SW480 (B) and A-431 (C) nuclear extracts and Hs 294T (D) and T84 (E) whole cell lysates.

galectin-3 (A3A12) - sc-53127 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
galectin-3 (A3A12): sc-53127. Western blot analysis of galectin-3 expression in HeLa (A), MCF7 (B), SW480 (C), WEHI-231 (D) and A-431 (E) nuclear extracts and Hs 294T (F) and T98G (G) whole cell lysates.

galectin-3 (M3/38.1.2.8 HL.2) - sc-81728 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
galectin-3 (M3/38.1.2.8 HL.2): sc-81728. Western blot analysis of galectin-3 expression in non-transfected 293T: sc-117752 (A), human galectin-3 transfected 293T: sc-116566 (B) and HeLa (C) whole cell lysates.

Anti-Galectin 3 antibody - ab53082 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53082 at 1/50 dilution, with and without blocking peptide (ab53082 immunogen).

Anti-Galectin 3 antibody - ab118851 from Abcam

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Galectin 3 antibody (ab118851) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HeLA cell extract with synthesized peptide

Anti-Galectin 3 antibody - ab31707 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Galectin 3 antibody (ab31707) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-Galectin 3 antibody - ab190167 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling Galectin 3 with ab190167 at 10 µg/ml.

Anti-Galectin 3 antibody - ab116708 from Abcam

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human colon tissue (formalin fixed, paraffin embedded) stained with ab116708 at a 1/50 dilution, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Galectin 3 antibody - ab109782 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Galectin 3 antibody - ab2473 from Abcam

Prices $372.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Galectin 3 antibody [9] - ab188166 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9
Anti-Galectin 3 antibody [9] (ab188166) at 1/1000 dilution + HeLa whole cell lysate

Anti-Galectin 3 antibody [9C4] - ab7278 from Abcam

Prices $378.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone 9C4
ICC/IF image of ab7278 stained PANC-1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7278 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- mouse (ab96879) IgG (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Galectin 3 antibody [A3A12] - ab2785 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone A3A12
ab2785 labelling Galectin 3 (green) in the cytoplasm and nucleus of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

Anti-Galectin 3 antibody (Biotin) - ab84238 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Galectin 3 antibody [EP2775Y] - ab76245 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2775Y
ICC/IF image of ab76245 stained Panc-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab76245 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Galectin 3 antibody [EPR2774] - ab76466 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2774
Overlay histogram showing THP1 cells stained with ab76466 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76466, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Galectin 3 antibody [M3/38] (Biotin) - ab95679 from Abcam

Prices $376.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone M3/38
ab95679 at 0.125µg dilution staining Galectin 3 in C57BL/6 mouse bone marrow cells by Flow Cytometry (shown in blue) followed by Streptavidin PE.

Anti-Galectin 3 antibody [M3/38] (Phycoerythrin) - ab95680 from Abcam

Prices $376.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone M3/38
ab95680 at 0.03µg dilution staining Galectin 3 in C57BL/6 bone marrow cells and anti-Mouse CD11b FITC by Flow Cytometry.
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