PRKDC

Gene Symbol PRKDC
Entrez Gene 5591
Alt Symbol DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350
Species Human
Gene Type protein-coding
Description protein kinase, DNA-activated, catalytic polypeptide
Other Description DNA-PK catalytic subunit|DNA-dependent protein kinase catalytic subunit|hyper-radiosensitivity of murine scid mutation, complementing 1|p460
Swissprots Q9UME3 Q7Z611 Q13327 Q59H99 Q96SE6 P78528 Q14175 P78527 Q13337
Accessions AAA79244 AAB51722 AAC52019 AAK40350 AAP69525 BAB79635 EAW86680 EAW86681 EAW86682 EAW86683 P78527 AB208860 BAD92097 AK296833 BAG59403 BC017494 BC024963 BC037968 U34994 AAC50210 U35835 AAA79184 U47077 AAB39925 XM_011517567 XP_011515869 XM_011517568 XP_011515870 NM_001081640 NP_001075109 NM_006904 NP_008835
Function Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylat
Subcellular Location Nucleus. Nucleus, nucleolus.
Top Pathways Cell cycle, Non-homologous end-joining

Phospho-DNA-PK (Ser2056) Antibody - 4215 from Cell Signaling Technology

Prices $279.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HCT 116 cells, untreated (-) or treated (+) with neocarzinostatin (NCS, 1 hr, 10 μM), using Phospho-DNA-PK (Ser2056) Antibody (upper) and DNA-PK Antibody #4602 (lower).

DNA-PKcs (3H6) Mouse mAb - 12311 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3H6
Western blot analysis of extracts from various cell lines using DNA-PKcs (3H6) Mouse mAb.

DNA-PKcs Antibody - 4602 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from M059K (DNA-PK wildtype) and M059J (DNA-PK deficient) cells, using DNA-PK Antibody.

DNA-PK CS (6) - sc-135886 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
DNA-PK<sub>CS</sub> (6): sc-135886. Western blot analysis of DNA-PK<sub>CS</sub> expression in HeLa whole cell lysate.

DNA-PK CS (E-3) - sc-390495 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DNA-PK<sub>CS</sub> (E-3): sc-390495. Western blot analysis of DNA-PK<sub>CS</sub> expression in HeLa whole cell lysate.

DNA-PK CS (E-6) - sc-390698 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DNA-PK<sub>CS</sub> (E-6): sc-390698. Western blot analysis of DNA-PK<sub>CS</sub> expression in CCRF-CEM whole cell lysate.

DNA-PK CS (G-4) - sc-5282 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DNA-PK<sub>CS</sub> (G-4): sc-5282. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.

DNA-PK CS (G-12) - sc-390849 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DNA-PK<sub>CS</sub> (G-12): sc-390849. Western blot analysis of DNA-PK<sub>CS</sub> expression in K-562 (A), MOLT-4 (B), HeLa (C) and CCRF-CEM (D) whole cell lysates.

DNA-PK CS (C-19) - sc-1552 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DNA-PK<sub>CS</sub> (C-19): sc-1552. Western blot analysis of DNA-PK<sub>CS</sub> expression in HeLa (A), MOLT-4 (B) and K-562 (C) whole cell lysates.

DNA-PK CS (H-163) - sc-9051 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DNA-PK<sub>CS</sub> (H-163): sc-9051. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing nuclear localization.

p-DNA-PK CS (Thr 2609) - sc-101664 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-DNA-PK<sub>CS</sub> (Thr 2609): sc-101664. Western blot analysis of phosphorylated DNA-PK<sub>CS</sub> expression in untreated (A) and hydroxyurea-treated (B) K-562 whole cell lysates.

Anti-DNA PKcs (phospho S2056) antibody - ab111399 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111399 at 1/50 dilution staining DNA PKcs in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with synthesized phosphopeptide.

Anti-DNA PKcs (phospho S2056) antibody [EPR5670] - ab124918 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5670
All lanes : Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918) at 1/1000 dilution (unpurified)Lane 1 : Jurkat cell lysate, untreatedLane 2 : Jurkat cell lysate, treated with alkaline phosphataseLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-DNA PKcs (phospho S2056) antibody - ChIP Grade - ab18192 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DNA PKcs (phospho S2056) antibody - ChIP Grade (ab18192) at 1 µg/mlLane 1 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) at 20 µgLane 2 : 20ug of untreated HeLa cell extractLane 3 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) at 20 µg with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/mlLane 4 : 20ug of untreated HeLa cell extract with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/mlLane 5 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) at 20 µg with Human DNA PKcs peptide (ab20407) at 1 µg/mlLane 6 : 20ug of untreated HeLa cell extract with Human DNA PKcs peptide (ab20407) at 1 µg/mlSecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DNA PKcs (phospho S2612) antibody - ab111605 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111605, at a 1/50 dilution, staining DNA PKcs in paraffin embedded Human brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-DNA PKcs (phospho S2612) antibody [EPR2251] - ab133441 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2251
All lanes : Anti-DNA PKcs (phospho S2612) antibody [EPR2251] (ab133441) at 1/1000 dilutionLane 1 : Lysate of LNCaP cells, untreated Lane 2 : Lysate of LNCaP cells treated with alkaline phosphataseLysates/proteins at 10 µg per lane.SecondaryHRP conjugated goat anti-rabbit antibody at 1/2000 dilution

Anti-DNA PKcs (phospho T2609) antibody - ab4194 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of DNA PKcs staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4194, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DNA PKcs (phospho T2609) antibody - ab97611 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DNA PKcs (phospho T2609) antibody (ab97611) at 1/1000 dilutionLane 1 : extract from K562 cells - untreatedLane 2 : extract from K562 cells treated with hydroxyurea

Anti-DNA PKcs (phospho T2609) antibody [10B1] - ab18356 from Abcam

Prices $392.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 10B1

Anti-DNA PKcs (phospho T2638) antibody - ab61046 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
1/500-1/1000 ab61046 staining phosphorilated DNA PKcs on HuvEc cells treated with 20% serum for 30 minutes.

Anti-DNA PKcs (phospho T2647) antibody - ab61045 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
1/500-1/1000 ab61045 staining phosphorilated DNA PKcs on HuvEc cells treated with 20% serum for 30 minutes.

Anti-DNA PKcs antibody - ab230 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab230 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab230, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DNA PKcs antibody - ab69527 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DNA PKcs antibody - ab133423 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Kidney tissue labelling DNA PKcs with ab133423 antibody at 5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-DNA PKcs antibody - ab53701 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab53701 at 1/50 dilution staining DNA PKcs in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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