GFAP

Gene Symbol GFAP
Entrez Gene 2670
Alt Symbol ALXDRD
Species Human
Gene Type protein-coding
Description glial fibrillary acidic protein
Other Description -
Swissprots Q7Z5J6 Q7Z5J7 Q53H98 E9PAX3 B2RD44 P14136 Q6ZQS3 Q96P18 D3DX59 Q9UFD0 Q5D055 Q96KS4
Accessions AAN87903 AAN87904 AAN87907 CAI99149 EAW51567 EAW51568 EAW51569 EAW51570 EAW51571 P14136 AF419299 AAL16662 AJ306447 CAC69881 AK098380 AK098758 AK124465 AK128790 BAC87610 AK222683 BAD96403 AK295734 BAG58573 AK296259 BAG58972 AK303728 BAG64704 AK315398 BAG37791 AL133013 CAB61354 BC012228 BC013596 AAH13596 BC041765 AAH41765 BC062609 AAH62609 BC127871 AAI27872 BF341765 BF526347 BM687259 BM931499 DA254392 DA315956 DQ979832 ABL14186 J04569 AAA52528 JF432461 ADZ15678 M26638 AAA52529 S40719 AAB22581 U92979 NM_001131019 NP_001124491 NM_001242376 NP_001229305 NM_002055 NP_002046
Function GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:12058025}. Note=Associated with intermediate filaments.
Tissue Specificity Expressed in cells lacking fibronectin. {ECO:0000269|PubMed:1847665}.

GFAP (GA5) Mouse mAb (Alexa Fluor ® 488 Conjugate) - 3655 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (100 tests)
Host Mouse
Clonality Monoclonal
Clone GA5
Confocal immunofluorescent analysis of rat brain using GFAP (GA5) Mouse mAb (Alexa Fluor ® 488 Conjugate) (green) and Neurofilament-L (DA2) Mouse mAb #2835 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

GFAP (GA5) Mouse mAb (Alexa Fluor ® 647 Conjugate) - 3657 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone GA5
Confocal immunofluorescent analysis of rat brain using GFAP (GA5) Mouse mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor) and Neurofilament-L (DA2) Mouse mAb #2835 (green).

GFAP (GA5) Mouse mAb (Alexa Fluor ® 555 Conjugate) - 3656 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone GA5
Confocal immunofluorescent analysis of normal rat cerebellum using GFAP (GA5) Mouse mAb (Alexa Fluor ® 555 Conjugate) (red) and Neurofilament-L (C28E10) Rabbit mAb #2837 (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

GFAP (GA5) Mouse mAb (Alexa Fluor ® 594 Conjugate) - 8152 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone GA5
Confocal Immunofluorescent analysis of rat cerebellum using GFAP (GA5) Mouse mAb (Alexa Fluor ® 594 Conjugate) (red) and Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor ® 488 Conjugate) #8024 (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

GFAP (D1F4Q) XP ® Rabbit mAb - 12389 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1F4Q
Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using GFAP (D1F4Q) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

GFAP (GA5) Mouse mAb - 3670 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone GA5
Western blot analysis of extracts from mouse and rat brain, using GFAP (GA5) Mouse mAb.

GFAP (2E1) - sc-33673 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GFAP (2E1): sc-33673. Western blot analysis of GFAP expression in non-transfected: sc-110760 (A) and human GFAP transfected: sc-111038 (B) 293 whole cell lysates.

GFAP (C-19) - sc-6170 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GFAP (C-19): sc-6170. Western blot analysis of GFAP expression in non-transfected: sc-110760 (A) and human GFAP transfected: sc-111038 (B) 293 whole cell lysates.

GFAP (F-2) - sc-166481 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GFAP (F-2): sc-166481. Western blot analysis of GFAP expression in non-transfected: sc-117752 (A) and human GFAP transfected: sc-115582 (B) 293T whole cell lysates.

GFAP (F-7) - sc-166458 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GFAP (F-7): sc-166458. Western blot analysis of GFAP expression in non-transfected: sc-117752 (A) and human GFAP transfected: sc-115582 (B) 293T whole cell lysates.

GFAP (GA-5) - sc-58766 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GFAP (GA-5): sc-58766. Western blot analysis of GFAP expression in non-transfected: sc-110760 (A) and human GFAP transfected: sc-111038 (B) 293 whole cell lysates.

GFAP (H-50) - sc-9065 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GFAP (H-50): sc-9065. Western blot analysis of GFAP expression in mouse brain tissue extract.

GFAP (N-18) - sc-6171 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GFAP (N-18) PE: sc-6171 PE. Intracellular FCM analysis of fixed and permeabilized SK-N-SH cells. Black line histogram represents the isotype control, normal goat IgG: sc-3992.

GFAP (SPM248) - sc-56395 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GFAP (SPM248): sc-56395. Western blot analysis of GFAP expression in C6 whole cell lysate.

GFAP (GF5) - sc-51908 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
GFAP (GF5): sc-51908. Western blot analysis of GFAP expression in non-transfected: sc-110760 (A) and human GFAP transfected: sc-111038 (B) 293 whole cell lysates.

GFAP (52) - sc-135921 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
GFAP (52): sc-135921. Western blot analysis of GFAP expression in SW-13 whole cell lysate.

GFAP (1.BB.807) - sc-71143 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
GFAP (1.BB.807): sc-71143. Western blot analysis of GFAP expression in non-transfected: sc-110760 (A) and human GFAP transfected: sc-111038 (B) 293 whole cell lysates.

GFAP (2A5) - sc-65343 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
GFAP (2A5): sc-65343. Western blot analysis of GFAP expression in non-transfected: sc-110760 (A) and human GFAP transfected: sc-111038 (B) 293 whole cell lysates.

p-GFAP (Ser 13) - sc-32956 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-GFAP (Ser 13): sc-32956. Western blot analysis of GFAP phosphorylation in non-transfected: sc-117752 (A) and human GFAP transfected: sc-115582 (B) 293T whole cell lysates.

Anti-GFAP (phospho S38) antibody - ab62479 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62479, at 1/50 dilution, staining GFAP in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

Anti-GFAP antibody - ab50738 from Abcam

Prices $379.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal
ICC/IF image of ab50738 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab50738 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GFAP antibody [6A6] - ab106509 from Abcam

Host Mouse
Clonality Monoclonal
Clone 6A6
Overlay histogram showing SHSY-5Y cells stained with ab106509 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab106509, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-GFAP antibody [6F2] - ab8975 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6F2
GFAP immunogold labelling TEM of corticobasal degeneration brain tissue showing heavy and highly-specific labelling over a glial process. Bar = 100 nm.This picture was kindly supplied as part of the review submitted by Dr Julian Thorpe (University of Sussex).

Anti-GFAP antibody [6F2], prediluted - ab86144 from Abcam

Prices $372.00
Sizes 6 ml
Host Mouse
Clonality Monoclonal
Clone 6F2

Anti-GFAP antibody [EP672Y] - ab33922 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP672Y
ICC/IF image of ab33922 stained Rat primary mixed astrocytes culture. The cells were 100% Paraformaldehyde fixed and then incubated in 10% Serum / 0.1M PBS with 10% Donkey serum for 4h. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution.
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