POLR2A

Gene Symbol POLR2A
Entrez Gene 5430
Alt Symbol POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
Species Human
Gene Type protein-coding
Description polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Other Description DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1
Swissprots A6NN93 Q6NX41 B9EH88 P24928
Accessions CAA52862 EAW90181 EAW90182 EAW90183 P24928 AK293777 BAG57192 AK304882 BAG65619 AW589665 BC067295 AAH67295 BC107140 BC137231 AAI37232 BQ353795 BQ891728 BU730469 BU739765 CV355924 DA923649 M21610 AAA60135 X63564 CAA45125 NM_000937 NP_000928
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA h
Subcellular Location Nucleus {ECO:0000269|PubMed:9852112}.
Top Pathways Huntington's disease, Herpes simplex infection, Epstein-Barr virus infection, Purine metabolism, RNA polymerase

Phospho-Rpb1 CTD (Ser2/Ser5) (D1G3K) Rabbit mAb - 13546 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1G3K
Western blot analysis of extracts from C2C12, H-4-II-E, and COS-7 cells using Phospho-Rpb1 CTD (Ser2/Ser5) (D1G3K) Rabbit mAb.

Phospho-Rpb1 CTD (Ser5) (D9N5I) Rabbit mAb - 13523 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9N5I
Western blot analysis of extracts from C2C12, H-4-II-E, and COS-7 cells using Phospho-Rpb1 CTD (Ser5) (D9N5I) Rabbit mAb.

Phospho-Rpb1 CTD (Ser2/5) Antibody - 4735 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MCF-7 cells, untreated or treated with doxorubicin (0.5 μM, 36 h), doxorubicin followed by λ Phosphatase NEB#P0753 (10,000 Units/ml, 1 h), nocodazole (50 ng/ml, 36 h) or nocodazole followed by λ Phosphatase, using Phospho-Rpb1 CTD (Ser2/5) Antibody (upper) or p53 Antibody #9282 (lower).

Phospho-Rpb1 CTD (Ser2) (E1Z3G) Rabbit mAb - 13499 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1Z3G
Western blot analysis of extracts from C2C12, H-4-II-E, and COS-7 cells using Phospho-Rpb1 CTD (Ser2) (E1Z3G) Rabbit mAb.

Phospho-Rpb1 CTD (Ser7) (E2B6W) Rabbit mAb - 13780 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E2B6W
Western blot analysis of extracts from various cell lines using Phospho-Rpb1 CTD (Ser7) (E2B6W) Rabbit mAb.

Rpb1 CTD (4H8) Mouse mAb - 2629 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 4H8
Western blot analysis of extracts from various cell lines using Rpb1 CTD (4H8) Mouse mAb.

Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred) - 8860 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred).

Phospho-Rpb1 CTD (Ser5) Antibody - 8807 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Phospho-Rpb1 CTD antibody specificity is demonstrated using peptide dot blot. Antibody binding to pre-coated Rpb1 CTD peptides is shown using Phospho-Rpb1 CTD (Ser2) Antibody #8798, Phospho-Rpb1 CTD (Ser5) Antibody #8807, a phospho-Rpb1 CTD (Ser7) antibody, and Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred) #8860. As expected, the Phoshpo-Rpb1 CTD (Ser5) Antibody #8807 only binds to the phospho-Rpb1 CTD peptide that is phosphorylated at Ser5.

Phospho-Rpb1 CTD (Ser2) Antibody - 8798 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Phospho-Rpb1 CTD antibody specificity is demonstrated using peptide dot blot. Antibody binding to pre-coated Rpb1 CTD peptides is shown using Phospho-Rpb1 CTD (Ser2) Antibody #8798, Phospho-Rpb1 CTD (Ser5) Antibody #8807, a phospho-Rpb1 CTD (Ser7) antibody, and Phospho-Rpb1 CTD (Ser2/Ser5) Antibody (IP Preferred) #8860. As expected, the Phospho-Rpb1 CTD (Ser2) Antibody #8798 only binds to the phospho-Rpb1 CTD peptide that is phosphorylated at Ser2.

Pol II (1.BB.61) - sc-71917 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Pol II (1.BB.61): sc-71917. Western blot analysis of Pol II expression in A-431 (A), A-673 (B), HeLa (C) and NIH/3T3 (D) nuclear extracts and HeLa whole cell lysate (E).

Pol II (3WG2) - sc-21750 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pol II (3WG2): sc-21750. Western blot analysis of Pol II expression in A-431 (A), U-2 OS (B), NIH/3T3 (C) and PC-12 (D) whole cell lysates.

Pol II (8WG16) - sc-56767 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Pol II (8WG16): sc-56767. Western blot analysis of Pol II expression in A-431 (A), A-673 (B), HeLa (C) and NIH/3T3 (D) nuclear extracts and HeLa whole cell lysate (E).

Pol II (A-10) - sc-17798 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pol II siRNA (h): sc-36290. Western blot analysis of Pol II expression in non-transfected control (A) and Pol II siRNA transfected (B) HeLa cells. Blot probed with Pol II (A-10): sc-17798. α-actinin (H-2): sc-17829 used as specificity and loading control.

Pol II (C-18) - sc-5943 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pol II (C-18): sc-5943. Western blot analysis of Pol II expression in A-673 (A), U-2 0S (B), A-431 (C) and NIH/3T3 (D) whole cell lysates.

Pol II (C-21) - sc-900 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of Pol II expression in A-431 (A,D), AT-3 (B,E) and A-673 (C,F) nuclear extracts. Antibodies tested include Pol II (C-21): sc-900 (A-C) and Pol II (N-20): sc-899 (D-F).

Pol II (CTD4H8) - sc-47701 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pol II (CTD4H8): sc-47701. Western blot analysis of Pol II expression in HeLa (A), A-431 (B) and PC-12 (C) whole cell lysates.

Pol II (F-12) - sc-55492 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pol II (F-12): sc-55492. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Pol II (H-224) - sc-9001 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ChIP analysis of transcription factor binding to the Interferon β promoter before (A) and six hours after (B) Sendai virus infection of HeLa cells. Antibodies tested included NFκB p65 (A): sc-109, ATF-2 (N-96): sc-6233, GCN5 (N-18): sc-6303, Pol II (H-224): sc-9001, TFIID (TBP)(SI-1): sc-273 and CBP (A-22): sc-369. Data kindly provided by G. Mosialos.

Pol II (mara-3) - sc-65884 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pol II (mara-3): sc-65884. Western blot analysis of Pol II expression in PC-12 (A) and HeLa (B) whole cell lysates.

Pol II (N-20) - sc-899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Pol II (N-20): sc-899. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

Pol III RPC39 (C39-1) - sc-21753 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pol III (C39-1): sc-21753. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon tissue showing nuclear localization.

Pol III RPC39 (C39-2) - sc-23913 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pol III RPC39 siRNA (h): sc-36292. Western blot analysis of Pol III RPC39 expression in non-transfected control (A) and Pol III RPC39 siRNA transfected (B) HeLa cells. Blot probed with Pol III RPC39 (C39-2): sc-23913. GAPDH (FL-335): sc-25778 used as specificity and loading control.

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S1801) antibody [EP1510Y] - ab76292 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1510Y
All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S1801) antibody [EP1510Y] (ab76292) at 1/200000 dilutionLane 1 : Human brain lysates, untreatedLane 2 : Human brain lysates treated with APLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/1000 dilution

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S1801) antibody [EP1510Y] (HRP) - ab195374 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1510Y
IHC image of RNA polymerase II CTD repeat YSPTSPS staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195374 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ab52208 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image shows human breast carcinoma tissue stained with ab52208 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.
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