GATA1

Gene Symbol GATA1
Entrez Gene 2623
Alt Symbol ERYF1, GATA-1, GF-1, GF1, NF-E1, NFE1, XLANP, XLTDA, XLTT
Species Human
Gene Type protein-coding
Description GATA binding protein 1 (globin transcription factor 1)
Other Description GATA-binding factor 1|NF-E1 DNA-binding protein|erythroid transcription factor|erythroid transcription factor 1|globin transcription factor 1|nuclear factor, erythroid 1|transcription factor GATA1
Swissprots Q96GB8 P15976
Accessions AGN75067 EAW50749 EAW50750 EAW50751 P15976 AI057349 BC009797 AAH09797 M30601 AAA35885 X17254 CAA35120 XM_011543897 XP_011542199 XM_011543898 XP_011542200 NM_002049 NP_002040
Function Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. {ECO:0000269|PubMed:22235304}.
Subcellular Location Nucleus.
Tissue Specificity Erythrocytes. {ECO:0000269|PubMed:8524811}.

GATA-1 (D52H6) XP ® Rabbit mAb (PE Conjugate) - 13353 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D52H6
Flow cytometric analysis of HeLa (blue) and K-562 (green) cells using GATA-1 (D52H6) XP ® Rabbit mAb (PE Conjugate).

GATA-1 (D24E4) XP ® Rabbit mAb - 4589 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D24E4
Western blot analysis of lysates from K-562, HEL and HeLa cells using GATA-1 (D24E4) XP ® Rabbit mAb. * putative GATA-1 fragment.

GATA-1 (D52H6) XP ® Rabbit mAb - 3535 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D52H6
Western blot analysis of lysates from various cell lines using GATA-1 (D52H6) XP ® Rabbit mAb.

GATA-1 Antibody - 4591 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of total lysates from K562 and HEL cells using GATA-1 Antibody.

GATA-1 (C-20) - sc-1233 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GATA-1 (C-20): sc-1233. Western blot analysis of GATA-1 expression in K-562 nuclear extract (A) and HEL 92.1.7 whole cell lysate (B).

GATA-1 (H-200) - sc-13053 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GATA-1 (H-200): sc-13053. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic staining of poietic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

GATA-1 (M-20) - sc-1234 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GATA-1 (M-20): sc-1234. Western blot analysis of GATA-1 expression in K-562 nuclear extract (A) and HEL 92.1.7 whole cell lysate (B).

GATA-1 (N1) - sc-266 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
GATA-1 (N1): sc-266. Western blot analysis of GATA-1 expression in K-562 nuclear extract.

GATA-1 (N6) - sc-265 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
GATA-1 (N6): sc-265. Western blot analysis of GATA-1 expression in NIH/3T3 nuclear extract.

p-GATA-1 (Ser 142) - sc-101686 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-GATA-1 (Ser 142): sc-101686. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.

p-GATA-1 (Ser 310) - sc-101687 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-GATA-1 (Ser 310): 101687. Western blot analysis of phosphorylated GATA-1 expression in untreated (A) and EPO-treated (B) COS7 whole cell lysates.

Anti-GATA1 (phospho S142) antibody - ab28816 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab28816 at (1:50-1:100), staining paraffin embedded human breast carcinoma. Left: Using GATA1 antibody (ab28816); Right: antibody preincubated with synthesized phosphopeptide.

Anti-GATA1 (phospho S310) antibody - ab194912 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GATA1 (phospho S310) antibody (ab194912) at 1/500 dilutionLane 1 : HT29 cell extract, untreatedLane 2 : HT29 cell extract, treated with Anisomycin

Anti-GATA1 (phospho S310) antibody - ab36719 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab36719 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36719, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GATA1 (phospho S310) antibody - ab192185 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GATA1 (phospho S310) antibody (ab192185) at 1/500 dilutionLane 1 : EPO treated COS7 whole cell lysate with the antigen-specific peptideLane 2 : EPO treated COS7 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-GATA1 antibody - ab28839 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using GATA-1(Ab-1) Antibody (ab28839); Right: The same antibody preincubated with synthesized peptide.

Anti-GATA1 antibody - ab131456 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence staining of methanol fixed HeLa cells labelling GATA1 with ab131456 antibody at a dilution of 1/100.

Anti-GATA1 antibody - ab47490 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GATA1 antibody (ab47490) at 1/500 dilutionLane 1 : extracts from COS7 cells, untreatedLane 2 : extracts from COS7 cells with synthesized peptide at 1 µg/mlLysates/proteins at 30 µg per lane.SecondaryAlkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)

Anti-GATA1 antibody - ab89505 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-GATA1 antibody (ab89505) at 5 µg/ml + PC12 cell lysate at 50 µg

Anti-GATA1 antibody - ab85466 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GATA1 antibody (ab85466) at 1 µg/mlLane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissueLane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-GATA1 antibody - ab82899 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GATA1 antibody (ab82899) at 1 µg/ml + MCF7 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-GATA1 antibody - ab92891 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GATA1 antibody (ab92891) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : Jurkat cell lysateLane 4 : THP1 cell lysateLane 5 : 3T6 cell lysateLysates/proteins at 20 µg per lane.

Anti-GATA1 antibody - ab40847 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-GATA1 antibody (ab40847) at 0.3 µg/ml + Human PBMC lysate (35µg protein in RIPA buffer).

Anti-GATA1 antibody [4F5] - ab98953 from Abcam

Host Mouse
Clonality Monoclonal
Clone 4F5
Anti-GATA1 antibody [4F5] (ab98953) at 1/500 dilution + K562 cell lysate

Anti-GATA1 antibody [EP2819Y] - ab76121 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2819Y
Anti-GATA1 antibody [EP2819Y] (ab76121) at 1/2000 dilution + K562 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/1000 dilution
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