POLH

Gene Symbol POLH
Entrez Gene 5429
Alt Symbol RAD30, RAD30A, XP-V, XPV
Species Human
Gene Type protein-coding
Description polymerase (DNA directed), eta
Other Description DNA polymerase eta|RAD30 homolog A|xeroderma pigmentosum variant type protein
Swissprots Q9Y253 Q9BX13 Q96BC4 Q7L8E3
Accessions AAQ81300 ACQ91143 BAB18601 EAX04209 EAX04210 EAX04211 EAX04212 Q9Y253 AB024313 BAA81666 AF158185 AAD43810 AF241258 AJ420484 AK023893 BAG51237 AK025631 AK122807 BAG53738 AK294430 BAG57675 AK307660 AK316151 BAH14522 BC015742 AAH15742 DC416114 XM_005249186 XP_005249243 XM_011514698 XP_011513000 NM_001291969 NP_001278898 NM_001291970 NP_001278899 NM_006502 NP_006493
Function DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. {ECO:0000269|PubMed:10385124, ECO:0000269|PubMed:11376341, ECO:0000269|PubMed:11743006, ECO:0000269|PubMed:14630940, ECO:0000269|PubMed:14734526}.
Subcellular Location Nucleus {ECO:0000269|PubMed:12606586}. Note=Accumulates at replication forks after DNA damage.
Top Pathways Fanconi anemia pathway

DNA Polymerase η (E1I7T) Rabbit mAb - 13848 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1I7T
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human POLH protein (hPOLH-Myc/DDK; +), using DNA Polymerase η (E1I7T) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

POL H (C-17) - sc-5938 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
POL H (C-17): sc-5938. Western blot analysis of C-terminal fragments of human recombinant POL H fusion protein.

POL H (B-7) - sc-17770 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
POL H (B-7): sc-17770. Western blot analysis of POL H expression in HeLa whole cell lysate.

POL H (H-300) - sc-5592 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
POL H (H-300): sc-5592. Western blot analysis of POL H expression in HeLa whole cell lysate.

Anti-DNA polymerase eta antibody - ab17725 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lanes 1, 2, 5 & 6 : Anti-DNA polymerase eta antibody (ab17725) at 1/400 dilutionLanes 3 - 4 : Anti-DNA polymerase eta antibody (ab17725) at 1/2000 dilutionLane 1 : XP30RO cell lysate (lacking endogenous DNA polymerase eta)Lane 2 : MRC5 cell lysateLane 3 : XP30RO cell lysate (lacking endogenous DNA polymerase eta)Lane 4 : MRC5 cell lysateLane 5 : XP30RO cell lysate(lacking endogenous DNA polymerase eta) with Human DNA polymerase eta peptide (ab19077) at 5 µlLane 6 : MRC5 cell lysate with Human DNA polymerase eta peptide (ab19077) at 5 µlSecondaryGoat anti-rabbit HRP at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DNA polymerase eta antibody - ab180703 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labelling DNA polymerase eta with ab180703 at 1/200. Magnification: 400x.

Anti-DNA polymerase eta antibody - ab154330 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft tissue, labeling DNA polymerase eta with ab154330 at 1/100 dilution.

Anti-DNA polymerase eta antibody - ab167307 from Abcam

Host Mouse
Clonality Polyclonal
All lanes : Anti-DNA polymerase eta antibody (ab167307) at 1 µg/mlLane 1 : DNA polymerase eta transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-DNA polymerase eta antibody - ab186677 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of U251MG cells (PFA-fixed/Triton X-100 permeabilized) labeling DNA polymerase eta with ab186677 at 4μg/ml.

Anti-DNA polymerase eta antibody - ab74223 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-POLH / DNA Polymerase Eta Antibody - LS-C192864 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of A431 cell lines, using POLH antibody.

Anti-POLH / DNA Polymerase Eta Antibody (aa68-92) - LS-C344594 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
POLH Antibody western blot of CEM cell lysate (35 ug/lane). This demonstrates that the POLH antibody detected the POLH protein (arrow).

Anti-POLH / DNA Polymerase Eta Antibody - LS-C335185 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded rat brain using POLH antibody at dilution of 1:200 (400x lens).

Anti-POLH / DNA Polymerase Eta Antibody (aa640-690) - LS-C64802 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-POLH / DNA Polymerase Eta Antibody (aa1-713) - LS-C128623 from LifeSpan Bioscience

Prices $445.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-POLH / DNA Polymerase Eta Antibody (aa690-713) - LS-C288040 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-POLH / DNA Polymerase Eta Antibody (aa640-690) - LS-C288038 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-POLH / DNA Polymerase Eta Antibody (aa690-713) - LS-C64803 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

XPV/DNA polymerase eta Antibody - AF3417 from Novus Biologicals

Prices $99.00, $329.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysates of MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPV Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3417) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPV at approximately 80 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.

XPV/DNA polymerase eta Antibody - NB100-60424 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

XPV/DNA polymerase eta Antibody - NBP1-87166 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

XPV/DNA polymerase eta Antibody - NBP1-87165 from Novus Biologicals

Prices $129.00, $419.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: XPV/DNA polymerase eta Antibody [NBP1-87165] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

XPV/DNA polymerase eta Antibody - H00005429-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: XPV/DNA polymerase eta Antibody [H00005429-B01P] - Analysis of POLH expression in transfected 293T cell line by POLH polyclonal antibody. Lane 1: POLH transfected lysate(78.43 KDa). Lane 2: Non-transfected lysate.

XPV/DNA polymerase eta Antibody - NB100-60423 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60423] - Detection of Human PolH by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PolH antibody NB100-60423 used for IP at 3 mcg/mg lysate. PolH was also immunoprecipitated by rabbit anti-PolH antibody NB100-60424, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

XPV/DNA polymerase eta Antibody - NBP2-32340 from Novus Biologicals

Prices $299.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
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