CD27

Gene Symbol CD27
Entrez Gene 939
Alt Symbol S152, S152. LPFS2, T14, TNFRSF7, Tp55
Species Human
Gene Type protein-coding
Description CD27 molecule
Other Description CD27 antigen|CD27L receptor|T cell activation antigen S152|T-cell activation antigen CD27|tumor necrosis factor receptor superfamily, member 7
Swissprots P26842 B2RDZ0
Accessions AAR84239 CBX47585 CBX53820 CCA78935 CDH61611 EAW88800 P26842 AK315732 BAG38087 BC012160 AAH12160 BI225100 DQ892296 ABM83222 EU176659 ABW03460 M63928 AAA58411 XM_011521042 XP_011519344 NM_001242 NP_001233
Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Subcellular Location Membrane; Single-pass type I membrane protein.
Tissue Specificity Found in most T-lymphocytes.
Top Pathways Cytokine-cytokine receptor interaction

CD27 (B-8) - sc-25289 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD27 (B-8): sc-25289. Western blot analysis of CD27 expression in Ramos (A), Caki-1 (B), SUP-T1 (C), TK-1 (D), IB4 (E) and KNRK (F) whole cell lysates.

CD27 (M-20) - sc-1743 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD27 (M-20): sc-1743. Immunofluorescence staining of methanol-fixed Ramos cells showing membrane localization.

CD27 (H-260) - sc-20923 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

CD27 (F-8) - sc-390079 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD27 (F-8): sc-390079. Western blot analysis of CD27 expression in TK-1 whole cell lysate.

CD27 (N-19) - sc-1740 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD27 (N-19): sc-1740. Western blot analysis of CD27 expression in Ramos whole cell lysate.

CD27 (M-T271) - sc-19653 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD27 (M-T271) FITC: sc-19653 FITC. FCM analysis of human peripheral blood leukocytes. Quadrant markers were set based on the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

Anti-CD27 antibody - ab175403 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling CD27 with ab175403 at 1/500. Magnification: 200x.

Anti-CD27 antibody - ab70103 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CD27 antibody (ab70103) at 1 µg/mlLane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 3 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-CD27 antibody - ab116560 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CD27 antibody (ab116560) at 1/100 dilution + Ramos cell lysate at 35 µg

Anti-CD27 antibody [137B4] - ab49518 from Abcam

Prices $379.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone 137B4
Human peripheral blood lymphocytes stained with ab49518 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab49518, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral

Anti-CD27 antibody [137B4], prediluted - ab75297 from Abcam

Prices $376.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone 137B4
Human tonsil tissue stained with ab75297 using a peroxidase-conjugate and AEC chromogen: Note membrane staining of T cells.

Anti-CD27 antibody [EPR8568] - ab133761 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8568
Anti-CD27 antibody [EPR8568] (ab133761) at 1/1000 dilution + Ramos cell lysate at 10 µgSecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-CD27 antibody [EPR8569] - ab131254 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8569
All lanes : Anti-CD27 antibody [EPR8569] (ab131254) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : Human lymph node cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Anti-CD27 antibody [LG.3A10] - ab80777 from Abcam

Prices $372.00
Sizes 125 µg
Clonality Monoclonal
Clone LG.3A10

Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free - ab185776 from Abcam

Prices $376.00
Sizes 50 µg
Clonality Monoclonal
Clone LG.3A10
Flow cytometric analysis of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.125 µg of ab185776 followed by Anti-Armenian Hamster IgG PE. Total viable cells were analyzed.

Anti-CD27 antibody [LG.7F9] - Low endotoxin, Azide free - ab171204 from Abcam

Prices $376.00
Sizes 500 µg
Clonality Monoclonal
Clone LG.7F9
Flow cytometric analysis of  BALB/c splenocytes with 0.25 µg Armenian Hamster IgG Isotype Control (open histogram) or 0.25 µg ab171204 (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were analyzed.

Anti-CD27 antibody [LT27] - ab1091 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone LT27

Anti-CD27 antibody [LT27] (FITC) - ab30366 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone LT27

Anti-CD27 antibody [LT27] (Phycoerythrin) - ab30367 from Abcam

Prices $378.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone LT27

Anti-CD27 antibody [LT27] (Phycoerythrin) - ab69773 from Abcam

Prices $376.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone LT27
ab69773 at 20µg/106 cells staining CD27 in human peripheral blood cells by flow cytometry.

Anti-CD27 antibody [MM0165-7H21] - ab89258 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM0165-7H21

Anti-CD27 antibody [O323] - ab134493 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone O323

Anti-CD27 antibody [O323] (Allophycocyanin) - ab134496 from Abcam

Prices $376.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone O323
Flow Cytometric analysis of normal Human peripheral blood cells labelling CD27 with either Mouse IgG1 K Isotype Control APC (open histogram) or ab134496 (5µl) (filled histogram). Cells in the lymphocyte gate were used for analysis.

Anti-CD27 antibody [O323] (Biotin) - ab95597 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone O323

Anti-CD27 antibody [O323] (FITC) - ab134494 from Abcam

Prices $376.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone O323
Flow Cytometric analysis of Human peripheral blood cells labelling CD27 with anti-Human CD19 APC and either Mouse IgG1 K Isotype Control FITC (left) or ab134494 (5µl) (right). Cells in the lymphocyte gate were used for analysis.
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