BTD

Gene Symbol	BTD
Entrez Gene	686
Alt Symbol	-
Species	Human
Gene Type	protein-coding
Description	biotinidase
Other Description	biotinase
Swissprots	F8W1Q3 B4DLJ9 P43251 B4DFX1 B2R865 A6NHF2 Q96EM9 B7Z7C9
Accessions	AAC21679 CCO13687 EAW64254 EAW64255 EAW64256 P43251 AI391654 AK294301 BAG57582 AK297033 BAG59561 AK301838 BAH13565 AK313252 BAG36062 AL573841 BC012099 AAH12099 BE814688 BE814713 BM565546 BM839350 BM919775 BP326502 U03274 AAC04318 XM_006713314 XP_006713377 XM_011534041 XP_011532343 NM_000060 NP_000051 NM_001281723 NP_001268652 NM_001281724 NP_001268653 NM_001281725 NP_001268654 NM_001281726 NP_001268655
Function	Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.
Subcellular Location	Secreted, extracellular space.
Top Pathways	Biotin metabolism, Vitamin digestion and absorption

biotinidase (H-97) - sc-368558 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

biotinidase (H-97): sc-368558. Western blot analysis of biotinidase expression in MOLT-4 (A), Jurkat (B), HeLa (C), NCI-H292 (D) and ACHN (E) whole cell lysates and mouse liver tissue extract (F).

biotinidase (K-17) - sc-102344 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

biotinidase (S-12) - sc-102346 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

Anti-BTD antibody - ab166934 from Abcam

Prices	$370.00
Sizes	200 µl
Host	Goat
Clonality	Polyclonal

Anti-BTD antibody (ab166934) at 0.5 µg/ml + Human liver lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-BTD antibody - ab54886 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Western blot against tagged recombinant protein immunogen using ab54886 BTD antibody at 1ug/ml.

Anti-BTD antibody - ab126140 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-BTD antibody (ab126140) at 1/1000 dilution + Jurkat lysate at 30 µg

Anti-BTD / Biotinidase Antibody - LS-C309643 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-BTD / Biotinidase Antibody - LS-C152808 from LifeSpan Bioscience

Prices	$365.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-BTD / Biotinidase Antibody - LS-C335648 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various tissues, using BTD antibody.

Anti-BTD / Biotinidase Antibody (aa372-385) - LS-C186449 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

BTD antibody (0.5 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BTD / Biotinidase Antibody (clone 3B10-2B3) - LS-C196774 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3B10-2B3

Western Blot analysis of BTD expression in transfected 293T cell line by BTD monoclonal antibody (M01), clone 3B10-2B3.Lane 1: BTD transfected lysate(61.1 KDa).Lane 2: Non-transfected lysate.

Biotinidase/BTD Antibody - NBP2-14891 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Biotinidase/BTD Antibody [NBP2-14891] - A: Jurkat.

Biotinidase/BTD Antibody - NBP2-33644 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry-Paraffin: BTD Antibody [NBP2-33644] - Staining of human colon shows strong cytoplasmic positivity in endothelial cells.

Biotinidase/BTD Antibody (3B10-2B3) - H00000686-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3B10-2B3

Western Blot: Biotinidase/BTD Antibody (3B10-2B3) [H00000686-M01] - Analysis of BTD expression in transfected 293T cell line by BTD monoclonal antibody (M01), clone 3B10-2B3.Lane 1: BTD transfected lysate(61.1 KDa).Lane 2: Non-transfected lysate.

Biotinidase/BTD Antibody - H00000686-D01P from Novus Biologicals

Prices	$399.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: Biotinidase/BTD Antibody [H00000686-D01P] - Analysis of BTD expression in transfected 293T cell line by BTD polyclonal antibody.Lane 1: BTD transfected lysate(61.10 KDa).Lane 2: Non-transfected lysate.

Biotinidase/BTD Antibody - H00000686-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: BTD Antibody [H00000686-B01] - analysis of BTD expression in transfected 293T cell line by BTD MaxPab polyclonal antibody (B01). Lane 1: BTD transfected lysate (61.10 KDa). Lane 2: Non-transfected lysate.

Biotinidase/BTD Antibody - NBP2-26186 from Novus Biologicals

Prices	$299.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Western Blot: Biotinidase/BTD Antibody [NBP2-26186] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Biotinidase/BTD Antibody - H00000686-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Monoclonal Anti-BTD antibody produced in mouse - WH0000686M1 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3B10-2B3

<B>Immunoblotting</B><BR/>Monoclonal Anti-BTD, <B>Cat. No. WH0000686M1</B> used at the antibody concentration: 1 μg/mL. Specific band of ~85.47 kDa using immunogen protein lysate.

Anti-BTD (506-520) antibody produced in rabbit - B7812 from Sigma-Aldrich

Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>Anti-BTD (506-520): <B>Cat. No. B7812</B>: Immunofluorescence of HUVEC cells using BTD (506-520) (RB),<B>Cat. No. B7812</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-BTD antibody produced in rabbit - SAB1411322 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-BTD: <B>Cat. No. SAB1411322</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~61.1 kDa using BTD transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-BTD (316-320) antibody produced in rabbit - B7687 from Sigma-Aldrich

Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>Anti-BTD (316-330): <B>Cat. No. B7687</B>: Immunofluorescence of HUVEC cells using BTD (316-330) (RB),<B>Cat. No. B7687</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-BTD antibody produced in goat - SAB2502164 from Sigma-Aldrich

Prices	$376.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-BTD<B>Cat. No. SAB2502164</B>: Immunoblotting (0.5 μg/mL) staining of Human Liver lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

biotinidase-aa372-385-Antibody-internal-region - AF3930a from Abgent, a WuXi AppTec company

Prices	$335.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

AF3930a (0.5 µg/ml) staining of Human Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti -BTD (Biotinidase, Biotinase, BTD) - 221119 from USBiological Life Science

Host	Rabbit
Clonality	Polyclonal

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