CDKN1C

Gene Symbol CDKN1C
Entrez Gene 1028
Alt Symbol BWCR, BWS, KIP2, WBS, p57, p57Kip2
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Other Description cyclin-dependent kinase inhibitor 1C|cyclin-dependent kinase inhibitor p57
Swissprots P49918
Accessions AAB05896 BAA11014 BAA11015 EAX02524 EAX02525 P49918 BC039188 BC067842 AAH67842 BM673714 CD676249 EU570054 ACB69757 U22398 AAA85095 XM_005252732 XP_005252789 XM_006725126 XP_006725189 NM_000076 NP_000067 NM_001122630 NP_001116102 NM_001122631 NP_001116103
Function Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non- proliferative state throughout life.
Subcellular Location Nucleus {ECO:0000250}.
Tissue Specificity Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver. {ECO:0000269|PubMed:22634751}.
Top Pathways Cell cycle

Phospho-p57 Kip2 (Thr310) Antibody - 2558 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated and dexamethasone-treated (50 nM, 16 h) HeLa cells, using Phospho-p57 Kip2 (Thr310) Antibody (upper) and p57 Kip2 Antibody #2557 (lower). Lysates were treated with lambda phosphatase NEB#P0753 (10,000 U/ml for 30 mins) to confirm the phospho-specificity of the phospho-antibody.

p57 Kip2 Antibody - 2557 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or treated with dexamethasone (50 nM, 16h) alone or with λ phosphatase, using p57 Kip2 Antibody #2557.

p57 (C-20) - sc-1040 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p57 (C-20)-G: sc-1040-G. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human fetal kidney showing nuclear staining.

p57 (H-91) - sc-8298 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p57 (KP39) - sc-56341 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p57 (KP39): sc-56341. Western blot analysis of p57 expression in A-673 whole cell lysate.

p57 (M-20) - sc-1039 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p57 (3F245) - sc-71823 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p57 (3F245): sc-71823. Western blot analysis of p57 expression in A-673 whole cell lysate.

p57 (SPM308) - sc-56456 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p57 (SPM308): sc-56456. Western blot analysis of p57 expression in A-673 whole cell lysate.

p57 (3F244) - sc-71824 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p57 (3F244): sc-71824. Western blot analysis of p57 expression in A-673 whole cell lysate.

Anti-p57 Kip2 (acetyl K278) antibody - ab190603 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-p57 Kip2 (acetyl K278) antibody (ab190603) at 1/500 dilution + 4T1 cell extract

Anti-p57 Kip2 (phospho T310) antibody - ab61064 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab61064 at 1/50-1/100 dilution with or without addition of Phospho-peptide.

Anti-p57 Kip2 antibody - ab61180 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61180 at 1/500 - 1/1000 dilution staining p57 Kip2 in HuvEc cells, treated with serum (20%, 15mins) by Immunofluorescence.

Anti-p57 Kip2 antibody - ab99888 from Abcam

Prices $376.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
Staining of Human p57 Kip2 in a formalin fixed, paraffin embedded sections using ab99888 at a dilution of 1/100.

Anti-p57 Kip2 antibody [3E3] - ab107224 from Abcam

Host Mouse
Clonality Monoclonal
Clone 3E3
All lanes : Anti-p57 Kip2 antibody [3E3] (ab107224) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : p57 Kip2-hIgGFc transfected HEK293 cell lysate

Anti-p57 Kip2 antibody [EP2515Y] - ab75974 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2515Y
Anti-p57 Kip2 antibody [EP2515Y] (ab75974) at 1/500 dilution (purified) + Human brain at 20 µgSecondaryHRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Anti-p57 Kip2 antibody [EP2515Y] (Alexa Fluor® 488) - ab199069 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2515Y
Overlay histogram showing HeLa cells stained with ab199069 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab199069, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-p57 Kip2 antibody [EP2515Y] (Alexa Fluor® 647) - ab199253 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2515Y
Overlay histogram showing HeLa cells stained with ab199253 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab199253, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

Anti-p57 Kip2 antibody [EP2515Y] (HRP) - ab199254 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2515Y
All lanes : Anti-p57 Kip2 antibody [EP2515Y] (HRP) (ab199254) at 1/5000 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue LysateLane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-p57 Kip2 antibody [EP2516] - ab119989 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2516
All lanes : Anti-p57 Kip2 antibody [EP2516] (ab119989) at 1/1000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate, treated with Dexamethasone Lysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-p57 Kip2 antibody [EP2718(2)] - ab133531 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2718(2)
All lanes : Anti-p57 Kip2 antibody [EP2718(2)] (ab133531) at 1/1000 dilutionLane 1 : Lysate of HeLa cells treated with dexamethasone Lane 2 : HeLa cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.

Anti-p57 Kip2 antibody [KP39] - ab3223 from Abcam

Prices $372.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone KP39

Anti-p57 Kip2 antibody [KP39] (Biotin) - ab79468 from Abcam

Prices $372.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone KP39

Anti-p57 Kip2 antibody, prediluted - ab99886 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Staining of Human p57 Kip2 in a Formalin/PFA-fixed paraffin-embedded sections using ab99886.

Anti-p57 Kip2 antibody [SP118] - ab119699 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP118
ab119699 at 1/100 dilution staining p57 Kip2 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry.

Anti-p57 Kip2 antibody [SPM308] - ab125272 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone SPM308
Overlay histogram showing HeLa cells stained with ab125272 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125272, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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