SMPD1

Gene Symbol SMPD1
Entrez Gene 6609
Alt Symbol ASM, ASMASE, NPD
Species Human
Gene Type protein-coding
Description sphingomyelin phosphodiesterase 1, acid lysosomal
Other Description acid sphingomyelinase|sphingomyelin phosphodiesterase
Swissprots Q16837 A8K8M3 Q16841 P17406 E9PKS3 P17405 Q13811
Accessions AAA58378 AAA75008 AAA75009 AAL79527 CAA45145 CDM61370 EAW68726 EAW68727 EAW68728 EAW68729 P17405 AA746054 AB209775 BAD93012 AK292388 BAF85077 AY649987 AAV68198 BC041164 AAH41164 BI599208 BM686561 BU845962 HQ132745 ADW08999 HQ132746 ADW09000 HQ132747 ADW09001 HQ132748 ADW09002 M59916 AAA58377 X52678 CAA36901 X52679 CAA36902 X59960 CAA42584 XM_005253075 XP_005253132 XM_011520303 XP_011518605 XM_011520304 XP_011518606 XR_930886 NM_000543 NP_000534 NM_001007593 NP_001007594 NR_027400
Function Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity.
Subcellular Location Lysosome.
Top Pathways Lysosome, Sphingolipid signaling pathway, Sphingolipid metabolism

ASM Antibody - 3687 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from THP-1 and HL-60 cells, untreated (-) or treated overnight with TPA #9905 (+), using ASM Antibody.

ASM (H-181) - sc-11352 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ASM (H-181): sc-11352. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.

ASM (A-19) - sc-9815 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ASM (A-19): sc-9815. Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.

ASM (N-17) - sc-9816 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ASM (N-17): sc-9816. Western blot analysis of human recombinant ASM fusion protein.

Anti-Acid sphingomyelinase antibody [mAbcam74281] - ab74281 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam74281
All lanes : Anti-Acid sphingomyelinase antibody [mAbcam74281] (ab74281) at 5 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Acid sphingomyelinase antibody [mAbcam74281] (FITC) - ab150251 from Abcam

Host Mouse
Clonality Monoclonal
Clone mAbcam74281

Anti-Acid sphingomyelinase antibody - C-terminal - ab170579 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Flow cytometric analysis of K562 cells labeling Acid sphingomyelinase with ab170579 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-Sphingomyelinase antibody - C-terminal - ab200088 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-Sphingomyelinase antibody - C-terminal (ab200088) at 1/500 dilutionLane 1 : Total cell extracts from HeLaLane 2 : Total cell extracts from Jurkat

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa62-341, clone 3H7) - LS-C338823 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3H7
IHC of paraffin-embedded Human endometrium tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa390-419, PE) - LS-C269714 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa390-419, Azide-free, HRP) - LS-C269715 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD1 / Acid Sphingomyelinase Antibody (clone 4H2) - LS-C198226 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4H2
Western Blot analysis of SMPD1 expression in transfected 293T cell line by SMPD1 monoclonal antibody (M01), clone 4H2.Lane 1: SMPD1 transfected lysate(39.6 KDa).Lane 2: Non-transfected lysate.

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa319-579, Biotin) - LS-C297968 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa319-579, FITC) - LS-C305327 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa79-128) - LS-C205675 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SMPD1 / Acid Sphingomyelinase antibody Western Blot of THP-1. Antibody dilution: 1 ug/ml.

Anti-SMPD1 / Acid Sphingomyelinase Antibody - LS-C314059 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa390-419) - LS-C330162 from LifeSpan Bioscience

Prices $435.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa319-579) - LS-C292595 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant SMPD1 / Acid Sphingomyelinase.

Anti-SMPD1 / Acid Sphingomyelinase Antibody - LS-C334919 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using SMPD1 antibody.

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa62-341, clone 10C5) - LS-C339172 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10C5
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMPD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMPD1.

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa391-419) IHC-plus™ - LS-B10421 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-SMPD1 / Acid Sphingomyelinase antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-SMPD1 / Acid Sphingomyelinase Antibody - LS-C125890 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-SMPD1 / Acid Sphingomyelinase Antibody - LS-C144184 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SMPD1 / Acid Sphingomyelinase Antibody (aa390-419, AP) - LS-C269710 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD1 / Acid Sphingomyelinase Antibody - LS-C26834 from LifeSpan Bioscience

Prices $460.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of acid sphingomyelinase antibody on normal human brain lysate (7 ug/lane). Antibody used at 1 ug/ml (1) and 0.5 ug/ml (2) and detected using mouse anti-rabbit antibody at 1:75k dilution and visualized using Pierce West Femto substrate.
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