RPS27A

Gene Symbol RPS27A
Entrez Gene 6233
Alt Symbol CEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80
Species Human
Gene Type protein-coding
Description ribosomal protein S27a
Other Description 40S ribosomal protein S27a|epididymis luminal protein 112|ubiquitin C|ubiquitin and ribosomal protein S27a|ubiquitin carboxyl extension protein 80|ubiquitin-40S ribosomal protein S27a|ubiquitin-CEP80
Swissprots P62979
Accessions BAA25826 BAB79488 EAX00106 EAX00107 EAX00108 EAX00109 EAX00110 P62979 AB362574 BAF81539 AK093614 AK225079 AK225718 AK315695 BAG38058 AY952889 BC001392 AAH01392 BC042362 AAH42362 BC053371 AAH53371 BC066293 AAH66293 BF678026 BM907473 BQ276758 BQ421613 CK004647 CN344335 CR746519 DA527860 DB505702 EU794671 ACJ13725 HQ448720 ADQ33198 M10939 AAA36788 S79522 AAB21188 X63237 CAA44911 NM_001135592 NP_001129064 NM_001177413 NP_001170884 NM_002954 NP_002945
Function Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription fac
Subcellular Location Ubiquitin: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
Top Pathways Ribosome

Ubiquitin (P4D1) Mouse mAb (HRP Conjugate) - 14049 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone P4D1
Western blot analysis of HeLa cells, untreated (-) or treated with the 26S proteasome inhibitor MG-132 #2194 (50 µM, 90 min; +), using Ubiquitin (P4D1) Mouse mAb (HRP Conjugate).

Ubiquitin Antibody - 3933 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with the proteasome inhibitor MG132 (10 µM for 90 minutes), using Ubiquitin Antibody.

Ubiquitin (P4D1) Mouse mAb - 3936 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone P4D1
Western blot analysis of 293 and HeLa cells, untreated or treated with the 26S proteasome inhibitor MG132 (50 µM, 90 minutes), using Ubiquitin (P4D1) Mouse mAb.

Ub (P4G7) - sc-53509 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ub (P4G7): sc-53509. Western blot analysis of Ub expression in non-transfected: sc-117752 (A) and human Ub transfected: sc-111402 (B) 293T whole cell lysates.

Ub (1B4-UB) - sc-58449 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Ub (1B4-UB): sc-58449. Western blot analysis of Ub expression in Jurkat whole cell lysate (A) and human recombinant Ub fusion protein (B).

Ub (10C2-2) - sc-58448 from Santa Cruz Biotechnology

Host Mouse
Clonality Monoclonal

Ub (FL-76) - sc-9133 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Ub (FL-76): sc-9133. Western blot analysis of lysates from control Jurkat cells (A), Jurkat cells treated with the proteasome inhibitor Lactacystin (sc-3575) showing accumulation of ubiquitinated proteins (B) and human recombinant Ub (C).

Ub (L-14) - sc-34870 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ub (L-14): sc-34870. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

Ub+1 (40B3) - sc-58450 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Ub+1 (40B3): sc-58450. Western blot analysis of Ub+1 expression in non-transfected (A), human Ub transfected (B) and human Ub+1 transfected (C) Bosc23 whole cell lysates.

Ub-RPS27A (K-17) - sc-324463 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ub-RPS27A (K-17): sc-324463. Western blot analysis of Ub-RPS27A expression in K-562 (A) and HL-60 (B) whole cell lysates.

Anti-RPS27A antibody - ab57646 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab57646 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57646, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-RPS27A Antibody (clone 3E2-E6) - LS-C198130 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E2-E6
RPS27A monoclonal antibody (M01), clone 3E2-E6 Western Blot analysis of RPS27A expression in HL-60.

Anti-RPS27A Antibody - LS-C193038 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of HL-60 cell lines, using RPS27A antibody.

Anti-RPS27A Antibody - LS-C331829 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of HL-60 cell lines, using RPS27A antibody.

Anti-RPS27A Antibody (aa73-122) - LS-C135519 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RPS27A antibody LS-C135519 Western blot of 293T cell lysate.

Ubiquitin Antibody - NB300-129 from Novus Biologicals

Prices $329.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using NB300-129.

Ubiquitin Antibody (EP296Y) - NB110-57638 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP296Y
Western Blot: Ubiquitin Antibody (EP296Y) [NB110-57638] Hela cell lysate using a 1:2,000 dilution.

Ubiquitin Antibody (P4G7-H11) - NBP1-19306 from Novus Biologicals

Prices $279.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone P4G7-H11
Western Blot: Ubiquitin Antibody (P4G7-H11) [NBP1-19306] - Western blot analysis of Ubiquitin: Lane 1: MW marker, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5: 3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK

Ubiquitin Antibody - NBP2-30132 from Novus Biologicals

Prices $99.00, $349.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal

Ubiquitin Antibody (10C2-2) - NB100-1650 from Novus Biologicals

Prices $279.00
Sizes 1 ml
Host Mouse
Clonality Monoclonal
Clone 10C2-2
Western Blot: Ubiquitin Antibody (10C2-2) [NB100-1650] - Detection of Ubiquitin protein (10 and free (20 on cell lysate from Rat prostate.

Ubiquitin Antibody - H00006233-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Ubiquitin Antibody [H00006233-D01P] - Analysis of RPS27A expression in transfected 293T cell line by RPS27A polyclonal antibody.Lane 1: RPS27A transfected lysate(18.00 KDa).Lane 2: Non-transfected lysate.

Ubiquitin Antibody - NB100-92119 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: Ubiquitin Antibody [NB100-92119] - Paraffin-embedded human breast carcinoma tissue.

Ubiquitin Antibody (6C11.B3) - NBP1-18942 from Novus Biologicals

Prices $309.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6C11.B3

Ubiquitin Antibody (3E2-E6) - H00006233-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E2-E6

Ubiquitin Antibody (FPM1) - NBP1-22628 from Novus Biologicals

Prices $329.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone FPM1
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