Fib

Gene Symbol Fib
Entrez Gene 37662
Alt Symbol CG9888, Dmel\CG9888, Fibri, GCR-6, GCR6, Pen59C5, fib, pen59C5
Species Drosophila
Gene Type protein-coding
Description Fibrillarin
Other Description CG9888-PA|Fib-PA|fibrillarin
Swissprots Q8IGK5 Q24348 Q9W1V3
Accessions AAF46950 Q9W1V3 BT125830 ADR83715 NM_079093 NP_523817
Function S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L- methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity). {ECO:0000250}.
Subcellular Location Nucleus, nucleolus {ECO:0000250}. Note=Fibrillar region of the nucleolus. {ECO:0000250}.
Top Pathways Ribosome biogenesis in eukaryotes

Anti-Fibrillarin antibody [38F3] - ab4566 from Abcam

Prices $401.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone 38F3
Rat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixation and staining procedure (in protocol section).

Anti-Fibrillarin antibody - Nucleolar Marker - ab5821 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
The cells were 100% methanol fixed (5 min) and thenincubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37266, 1µg/ml, red) and (ab5821, 1µg/ml, blue) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. 

Anti-FIB monoclonal antibody, clone 5D4 - CABT-BL1517 from Creative Diagnostics

Host Mouse
Clonality Monoclonal
Clone 5D4

Fibrillarin, Polyclonal Antibody - MBS5304852 from MyBioSource

Prices $310.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066103 from MyBioSource

Prices $900.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066104 from MyBioSource

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066105 from MyBioSource

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066106 from MyBioSource

Prices $605.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066107 from MyBioSource

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066108 from MyBioSource

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066109 from MyBioSource

Prices $975.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066110 from MyBioSource

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066111 from MyBioSource

Prices $500.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066112 from MyBioSource

Prices $655.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066113 from MyBioSource

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Fibrillarin (FBL), Polyclonal Antibody - MBS2066114 from MyBioSource

Prices $440.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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