ANPEP

Gene Symbol ANPEP
Entrez Gene 290
Alt Symbol APN, CD13, GP150, LAP1, P150, PEPN
Species Human
Gene Type protein-coding
Description alanyl (membrane) aminopeptidase
Other Description AP-M|AP-N|alanyl aminopeptidase|aminopeptidase M|aminopeptidase N|hAPN|microsomal aminopeptidase|myeloid plasma membrane glycoprotein CD13
Swissprots P15144 Q16728 Q8IUK3 Q8IVH3 Q9UCE0 Q6GT90
Accessions AAA83399 AAQ09003 CAD19098 CAD19802 CAD19822 CAD20931 CDM22281 EAX02067 EAX02068 P15144 AB209918 BAD93155 AK298134 BAG60413 AK298242 BAG60508 AK298339 BAG60587 AK300946 BAG62575 BC058928 AAH58928 BQ879097 M22324 AAA51719 X13276 CAA31640 XM_005254892 XP_005254949 XM_011521473 XP_011519775 XM_011521474 XP_011519776 NM_001150 NP_001141
Function Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. {ECO:00
Subcellular Location Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytosol {ECO:0000305}. Note=A soluble form has also been detected.
Tissue Specificity Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood- brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
Top Pathways Hematopoietic cell lineage, Glutathione metabolism, Renin-angiotensin system

CD13/APN (E1Y7U) Rabbit mAb - 13721 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1Y7U
Western blot analysis of extracts from various tissues and cell lines using CD13/APN (E1Y7U) Rabbit mAb.

CD13 (B-F10) Mouse mAb - 3566 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone B-F10
Flow cytometric analysis of whole blood, using CD13 (B-F10) Mouse mAb.

CD13 (A-5) - sc-166270 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD13 (A-5): sc-166270. Western blot analysis of CD13 expression in CCD-1064Sk whole cell lysate.

CD13 (BR2) - sc-53970 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD13 (BR2): sc-53970. Indirect FCM analysis of human peripheral blood leukocytes stained with CD13 (BR2), followed by PE-conjugated goat anti-mouse IgG: sc-3738. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-3877.

CD13 (H-300) - sc-15360 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CD13 (H-300): sc-15360. Western blot analysis of CD13 expression in CCD-1064Sk whole cell lysate.

CD13 (C-17) - sc-6995 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD13 (C-17): sc-6995. Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.

CD13 (12) - sc-136484 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD13 (12): sc-136484. Western blot analysis of CD13 expression in rat kidney tissue extract.

CD13 (3D8) - sc-13536 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of CD13 expression in GBB whole cell lysate immunoprecipitated with CD13 (BR2): sc-53970 and detected with CD13 (3D8): sc-13536.

CD13 (B-F10) - sc-65292 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD13 (B-F10): sc-65292. Western blot analysis of CD13 expression in CCD-1064Sk whole cell lysate.

CD13 (H-8) - sc-166105 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD13 (H-8): sc-166105. Western blot analysis of CD13 expression in non-transfected 293T: sc-117752 (A), human CD13 transfected 293T: sc-116664 (B) and CCD-1064Sk (C) whole cell lysates.

CD13 (SJ1D1) - sc-18899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD13 (SJ1D1) FITC: sc-18899 FITC. FCM analysis of human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-2855.

CD13 (WM15) - sc-51522 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
CD13 (WM15): sc-51522. Indirect FCM analysis of human peripheral blood leukocytes stained with CD13 (WM15), followed by PE-conjugated goat anti-mouse IgG<sub>1</sub>: sc-3764. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-3877.

Anti-CD13 antibody - ab119940 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CD13 antibody (ab119940) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal Liver lysate

Anti-CD13 antibody - ab154116 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CD13 antibody (ab154116) at 1/500 dilution + Raji whole cell lysate at 30 µg

Anti-CD13 antibody - ab85254 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CD13 antibody (ab85254) at 1/50 dilution + SK-Br-3 cell line lysate at 35 µg

Anti-CD13 antibody - ab88649 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-CD13 antibody (ab88649) at 1 µg/ml + human liver tissue lysate at 50 µgdeveloped using the ECL technique

Anti-CD13 antibody - ab105623 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CD13 antibody (ab105623) at 1 µg/ml + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutionPerformed under reducing conditions.

Anti-CD13 antibody - ab32824 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CD13 antibody [22A5] - ab20136 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 22A5
Ab20136 staining Human normal colon. Staining is localized to the membrane.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-CD13 antibody [22A5] (Biotin) - ab25723 from Abcam

Prices $186.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 22A5

Anti-CD13 antibody [22A5] (FITC) - ab79151 from Abcam

Prices $186.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone 22A5

Anti-CD13 antibody [38C12] - ab86907 from Abcam

Host Mouse
Clonality Monoclonal
Clone 38C12
ab86907 staining CD13 in human small intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with Bouins and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 3% peroxidase for 5 minutes at 20°C followed by incubation with the primary antibody at a 1/250 dilution for 1 hour at 20°C. A undiluted HRP-conjugated goat anti-mouse polyclonal was used as the secondary antibody.See Abreview

Anti-CD13 antibody [B-F10] - ab46881 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone B-F10
A typical staining pattern with ab46881 monoclonal antibody of monocytes.

Anti-CD13 antibody [B-F10] - ab47203 from Abcam

Prices $376.00
Sizes 1 ml
Host Mouse
Clonality Monoclonal
Clone B-F10
A typical staining pattern using ab47203 of monocytes.

Anti-CD13 antibody [B-F10] (FITC) - ab46882 from Abcam

Prices $188.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone B-F10
A typical staining pattern with ab46882 monoclonal antibody of monocytes.
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