HACD1
| Gene Symbol | HACD1 |
|---|---|
| Entrez Gene | 9200 |
| Alt Symbol | CAP, PTPLA |
| Species | Human |
| Gene Type | protein-coding |
| Description | 3-hydroxyacyl-CoA dehydratase 1 |
| Other Description | cementum attachment protein|cementum-attachment protein|protein tyrosine phosphatase-like (proline instead of catalytic arginine), member A|very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1|very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 1 |
| Swissprots | B0YJ81 Q9UHX2 Q96FW7 Q6JIC5 B0YJ80 Q9HB93 |
| Accessions | AAG10713 ACA06058 ACA06059 ACA06060 ACA06061 B0YJ81 CAY55982 EAW86210 EAW86211 AF114494 AAF21976 AW022173 AY455942 AAR22554 BC010353 AAH10353 BC027709 HQ447343 ADQ31829 XM_005252641 XP_005252698 XR_428651 NM_014241 NP_055056 |
| Function | Isoform 1: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. {ECO:0000269|PubMed:18554506}. |
| Subcellular Location | Isoform 1: Endoplasmic reticulum membrane {ECO:0000269|PubMed:18554506}; Multi-pass membrane protein {ECO:0000269|PubMed:18554506}. |
| Tissue Specificity | Isoform 1 is highly expressed in the myocardium, and to a lesser extent in skeletal and smooth muscular tissues including those from stomach, jejunum, and bladder. Also detected in gingival fibroblasts, periodontal ligament cells, osteoblasts and cementoblasts (PubMed:11054553, PubMed:22067203). Isoform 2 is specifically expressed by cementoblasts but also detected in periodontal ligament cells, heart, liver and kidney (at protein level) (PubMed:22067203). {ECO:0000269|PubMed:11054553, ECO:0000269|PubMed:22067203}. |
| Top Pathways | Fatty acid elongation, Biosynthesis of unsaturated fatty acids, Fatty acid metabolism |
PTPLA CRISPR/Cas9 KO Plasmid (h) - sc-404833 from Santa Cruz Biotechnology
|
||||||||
PTPLA HDR Plasmid (h) - sc-404833-HDR from Santa Cruz Biotechnology
|
||||||||
PTPLA Double Nickase Plasmid (h) - sc-404833-NIC from Santa Cruz Biotechnology
|
||||||||
PTPLA Double Nickase Plasmid (h2) - sc-404833-NIC-2 from Santa Cruz Biotechnology
|
||||||||
PTPLA CRISPR Activation Plasmid (h) - sc-404833-ACT from Santa Cruz Biotechnology
|
||||||||
PTPLA CRISPR Activation Plasmid (h2) - sc-404833-ACT-2 from Santa Cruz Biotechnology
|
||||||||
PTPLA Lentiviral Activation Particles (h) - sc-404833-LAC from Santa Cruz Biotechnology
|
||||||||
PTPLA Lentiviral Activation Particles (h2) - sc-404833-LAC-2 from Santa Cruz Biotechnology
|
||||||||
PTPLA siRNA (h) - sc-90324 from Santa Cruz Biotechnology
|
||||||||
PTPLA shRNA Plasmid (h) - sc-90324-SH from Santa Cruz Biotechnology
|
||||||||
PTPLA shRNA (h) Lentiviral Particles - sc-90324-V from Santa Cruz Biotechnology
|
||||||||
PTPLA RNAi - H00009200-R01 from Novus Biologicals
|
||||||||
MISSION® esiRNA - EHU061291 from Sigma-Aldrich
|
||||||||
MISSION® Human Phosphatase - SIHP0709 from Sigma-Aldrich
|
||||||||
MISSION® Human Phosphatase - SIHP0710 from Sigma-Aldrich
|
||||||||
MISSION® Human Phosphatase - SIHP0711 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN17691 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17691 from Sigma-Aldrich
|
||||||||
MISSION® 3′UTR Lenti GoClone™ - HUTR03215 from Sigma-Aldrich
|
||||||||
PTPLA siRNA (Human) - CRH6087 from Cohesion Biosciences
|
||||||||
shRNA set against Human PTPLA (NM_014241.3) - SHH386364 from Creative biogene
|
||||||||
shRNA set against Human PTPLA(NM_014241.3) - SHR151608 from Creative biogene
|
||||||||
Human PTPLA siRNA - orb266589 from Biorbyt
|
||||||||
PTPLA shRNA Plasmid (Human) - CHH6087 from Cohesion Biosciences
|
||||||||
PTPLA miRNA 3'UTR clone - MiUTR1H-08443 from Creative biogene
|