AP2A2

Gene Symbol AP2A2
Entrez Gene 515216
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description adaptor-related protein complex 2, alpha 2 subunit
Other Description 100 kDa coated vesicle protein C|AP-2 complex subunit alpha-2|adapter-related protein complex 2 alpha-2 subunit|adapter-related protein complex 2 subunit alpha-2|adaptor protein complex AP-2 subunit alpha-2|adaptor-related protein complex 2 subunit alpha-2|alpha-adaptin C|alpha2-adaptin|clathrin assembly protein complex 2 alpha-C large chain|plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
Swissprots Q0VCK5
Accessions DAA13547 Q0VCK5 BC120121 AAI20122 NM_001075702 NP_001069170
Function Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to p
Subcellular Location Cell membrane {ECO:0000250}. Membrane, coated pit {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. {ECO:0000250}.
Top Pathways Huntington's disease, Endocytosis, Endocrine and other factor-regulated calcium reabsorption, Synaptic vesicle cycle

Anti-alpha Adaptin antibody [100/2] - ab11326 from Abcam

Host Mouse
Clonality Monoclonal
Clone 100/2

Anti-alpha Adaptin antibody [AC1-M11] - ab2807 from Abcam

Prices $388.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone AC1-M11
Immunohistochemistry was performed on normal biopsies of deparaffinized Human heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing alpha Adaptin (ab2807) or without primary antibody (negative control) overnight at 4�C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-alpha Adaptin antibody [AP6] - ab2730 from Abcam

Prices $403.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AP6
Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in U251 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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