FASN

Gene Symbol FASN
Entrez Gene 2194
Alt Symbol FAS, OA-519, SDR27X1
Species Human
Gene Type protein-coding
Description fatty acid synthase
Other Description short chain dehydrogenase/reductase family 27X, member 1
Swissprots Q13479 Q969R1 P49327 Q4LE83 Q96C68 Q6P4U5 Q6SS02 Q16702 Q96IT0
Accessions AAC50536 EAW89745 P49327 AB209988 BAE06070 AY451392 AAS09886 BC007267 AAH07267 BC007305 BC007909 AAH07909 BC014631 AAH14631 BC014634 AAH14634 BC021544 BC063242 AAH63242 S80437 AAB35516 U26644 AAC50259 U29344 AAA73576 XM_011523538 XP_011521840 NM_004104 NP_004095
Function Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:17081065}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity Ubiquitous. Prominent expression in brain, lung, and liver. {ECO:0000269|PubMed:7567999, ECO:0000269|PubMed:7595075}.
Top Pathways Insulin signaling pathway, AMPK signaling pathway, Fatty acid biosynthesis, Fatty acid metabolism

Fatty Acid Synthase Antibody - 3189 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, using Fatty Acid Synthase Antibody.

Fatty Acid Synthase (C20G5) Rabbit mAb - 3180 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C20G5
Western blot analysis of extracts from various cell types using Fatty Acid Synthase (C20G5) Rabbit mAb.

Fatty Acid Synthase (A-5) - sc-55580 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fatty Acid Synthase (A-5): sc-55580. Western blot analysis of Fatty Acid Synthase expression in c4 (A) and AT-3 (B) whole cell lysates.

Fatty Acid Synthase (C-20) - sc-16147 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of human recombinant Fatty Acid Synthase fusion protein (A,B). Antibodies tested include Fatty Acid Synthase (C-20): sc-16147 (A) and Fatty Acid Synthase (H-300): sc-20140 (B).

Fatty Acid Synthase (H-300) - sc-20140 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Fatty Acid Synthase (H-300): sc-20140. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer showing cytoplasmic staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Fatty Acid Synthase (G-11) - sc-48357 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fatty Acid Synthase (G-11): sc-48357. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Anti-Fatty Acid Synthase antibody - ab189090 from Abcam

Prices $384.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-Fatty Acid Synthase antibody (ab189090) at 1/1000 dilution + Ramos whole cell lysate

Anti-Fatty Acid Synthase antibody - ab96866 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Fatty Acid Synthase antibody (ab96866) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg

Anti-Fatty Acid Synthase antibody - ab99358 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Fatty Acid Synthase with ab99358 at 1/200 (1µg/ml). Detection: DAB.

Anti-Fatty Acid Synthase antibody - ab82370 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Fatty Acid Synthase antibody (ab82370) at 1/50 dilutionLane 1 : non transfected 293 cell lysateLane 2 : transiently transfected 293 cell lysateLysates/proteins at 2 µg per lane.

Anti-Fatty Acid Synthase antibody - ab71645 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Fatty Acid Synthase antibody (ab71645) at 1/500 dilution + Immunising protein at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

Anti-Fatty Acid Synthase antibody - ab110954 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab110954, at 3.75µg/ml staining Fatty Acid Synthase in formalin-fixed, paraffin-embedded Human Brain cerebellum by Immunohistochemistry.

Anti-Fatty Acid Synthase antibody [EPR7465] - ab128856 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7465
Overlay histogram showing A549 cells stained with ab128856 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128856, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Fatty Acid Synthase antibody [EPR7466] - ab128870 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7466
Overlay histogram showing A549 cells stained with ab128870 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128870, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Fatty Acid Synthase antibody [EPR7466] (Alexa Fluor® 488) - ab197485 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7466
Overlay histogram showing A549 cells stained with ab197485 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197485, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in A549 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Fatty Acid Synthase antibody [EPR7466] (Alexa Fluor® 647) - ab196853 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7466
ab196853 staining Fatty Acid Synthase in A549 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196853 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Fatty Acid Synthase antibody [EPR7466] (HRP) - ab196854 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7466
All lanes : Anti-Fatty Acid Synthase antibody [EPR7466] (HRP) (ab196854) at 1/5000 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Fatty Acid Synthase antibody [GT237] - ab184619 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT237
All lanes : Anti-Fatty Acid Synthase antibody [GT237] (ab184619) at 1/5000 dilutionLane 1 : PC-12 whole cell lysate extractLane 2 : Rat-2 whole cell lysate extractLysates/proteins at 30 µg per lane.

Anti-Fatty Acid Synthase antibody [GT5210] - ab184632 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT5210
All lanes : Anti-Fatty Acid Synthase antibody [GT5210] (ab184632) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-FASN / Fatty Acid Synthase Antibody (aa942-973) IHC-plus™ - LS-B3636 from LifeSpan Bioscience

Prices $395.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-Fatty Acid Synthase antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3636 concentration 10 ug/ml.

Anti-FASN / Fatty Acid Synthase Antibody (aa2263-2512, Biotin) - LS-C297202 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FASN / Fatty Acid Synthase Antibody (aa2257-2504, Biotin) - LS-C298408 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FASN / Fatty Acid Synthase Antibody (aa2263-2512, FITC) - LS-C302084 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FASN / Fatty Acid Synthase Antibody (aa2257-2504, FITC) - LS-C305099 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FASN / Fatty Acid Synthase Antibody (aa2263-2512) - LS-C293843 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant FASN / Fatty Acid Synthase.
Search more