HIST1H2BB

Gene Symbol HIST1H2BB
Entrez Gene 3018
Alt Symbol H2B.1, H2B/f, H2BFF
Species Human
Gene Type protein-coding
Description histone cluster 1, H2bb
Other Description H2B histone family, member F|histone 1, H2bb|histone H2B type 1-B|histone H2B.1|histone H2B.f
Swissprots P33778 Q4KN36
Accessions AAN06685 CAA40406 EAW55513 P33778 BC096728 AAH96728 EU446966 ABZ92495 NM_021062 NP_066406
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Location Nucleus. Chromosome.
Top Pathways Systemic lupus erythematosus, Viral carcinogenesis, Alcoholism

Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb - 6959 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D67H2
Western blot analysis of extracts from HL-60 cells, untreated or etoposide-treated (50 μM, 6 hr) using Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb (upper) or Histone H2B (53H3) Mouse mAb #2934 (lower). Antibody phospho-specificity was determined by λ phosphatase treatment.

Acetyl-Histone H2B (Lys5) (D5H1S) XP ® Rabbit mAb - 12799 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5H1S
Western blot analysis of extracts from HeLa and C6 cells, untreated (-) or treated with Trichostatin A (TSA) #9950 (1 μM, 18hr; +), using Acetyl-Histone H2B (Lys5) (D5H1S) XP ® Rabbit mAb (upper) or Histone H2B (D2H6) Rabbit mAb #12364 (lower).

Acetyl-Histone H2B (Lys15) (D8H1) XP ® Rabbit mAb - 9083 from Cell Signaling Technology

Prices $318.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8H1
Western blot analysis of extracts from HeLa and C2C12 cells, untreated (-) or treated (+) with Trichostatin A (TSA) #9950 (1 μM, 18 hr), using Acetyl-Histone H2B (Lys15) (D8H1) XP ® Rabbit mAb (upper) and Histone H2B (V119) Antibody #8135 (lower).

Acetyl-Histone H2B (Lys5) Antibody - 2574 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H2B (Lys5) Antibody.

Acetyl-Histone H2B (Lys20) Antibody - 2571 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H2B (Lys20) Antibody.

Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb - 9861 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7H4
Western blot analysis of extracts from HeLa and C2C12 cells, untreated (-) or treated (+) with Trichostatin A (TSA) #9950 (1 μM, 18 hr), using Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (upper) and Histone H2B (53H3) Mouse mAb #2934 (lower).

Acetyl-Histone H2B (Lys12) Antibody - 5410 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with Trichostatin A #9950 (1 μM for 18 h), using Acetyl-Histone H2B (Lys12) Antibody (upper) and Histone H2B (53H3) Mouse mAb #2934 (lower).

Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated) - 9072 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 immunoprecipitations)
Host Rabbit
Clonality Monoclonal
Clone D7H4
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 µl of Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated) or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, SimpleChIP ® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb - 5546 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D11
Western blot analysis of extracts from various cell lines using Ubiquityl-Histone H2B (Lys120) (D11) XP ® Rabbit mAb.

Histone H2B (53H3) Mouse mAb - 2934 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 53H3
Western blot analysis of extracts from various cell lines using Histone H2B (53H3) Mouse mAb.

Histone H2B (V119) Antibody - 8135 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Histone H2B (V119) Antibody.

Histone H2B (D2H6) Rabbit mAb - 12364 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2H6
Western blot analysis of extracts from various cell lines using Histone H2B (D2H6) Rabbit mAb.

Acetyl-Histone H2B (Lys15) Antibody - 5435 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or treated with Trichostatin A (TSA) #9950 (1 μM for 18 h), using Acetyl-Histone H2B (Lys15) Antibody (upper) and Histone H2B (53H3) Mouse mAb #2934 (lower).

Acetyl-Histone H2B (Lys12) Antibody - 2575 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H2B (Lys12) Antibody.

Histone H2B Antibody - 2722 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Anti-Histone H2B type 1B antibody - ab124054 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Histone H2B type 1B antibody (ab124054) at 1/100 dilution + Jurkat cell line lysates at 35 µg

Anti-Histone H2B type 1B antibody [EP2327] - ab76246 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2327
Anti-Histone H2B type 1B antibody [EP2327] (ab76246) at 1/1000 dilution + Jurkat cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/1000 dilution

Anti-HIST1H2BB Antibody (aa1-30) - LS-C203665 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of HIST1H2BB/HIST1H2BE (arrow) using rabbit polyclonal HIST1H2BB/HIST1H2BE Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HIST1H2BB/HIST1H2BE gene.

Histone H2B type 1B [ac Lys5, ac Lys12, ac Lys15, ac Lys20] Antibody - NB100-63300 from Novus Biologicals

Prices $329.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Histone H2B type 1B Antibody - NBP1-64754 from Novus Biologicals

Prices $229.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Histone H2B type 1B Antibody [NBP1-64754] - Analysis of extracts from Jurkat cells, treated with UV 15', using Histone H2B type 1B Antibody. The lane on the right is treated with the synthesized peptide.

Anti-Histone H2B, N-Terminal antibody produced in rabbit - SAB4501372 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-Histone H2B, N-Terminal: <B>Cat. No. SAB4501372</B>: Immunoblotting analysis of extracts from Jurkat cells, treated with UV 15′, using Anti-Histone H2B, N-Terminal antibody. The lane on the left was treated with the Anti-Histone H2B, N-Terminal antibody. The lane on the right (negative control) was treated with both Anti-Histone H2B, N-Terminal antibody and the synthesized immunogen peptide.

ANTI-HIST1H2BB/HIST1H2BE (N-TERM) antibody produced in rabbit - SAB1304961 from Sigma-Aldrich

Prices $326.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-HIST1H2BB/HIST1H2BE (N-term): <B>Cat. No. SAB1304961</B>: HIST1H2BB/HIST1H2BE Antibody (N-term) western blot analysis in Jurkat cell line lysates (35 μg/lane).

HIST1H2BBHIST1H2BE-Antibody-N-term - AP16190a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
HIST1H2BB/HIST1H2BE Antibody (N-term) (Cat. #AP16190a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HIST1H2BB/HIST1H2BE antibody detected the HIST1H2BB/HIST1H2BE protein (arrow).

Histone H2B antibody - GTX87385 from GeneTex

Prices $319.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Anti-Histone H2B antibody used in Western blot. GTX87385

Histone H2BK12ac (acetyl Lys12) antibody - GTX88011 from GeneTex

Prices $319.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Anti-Histone H2BK12ac (acetyl Lys12) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX88011
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