SMAD4
| Gene Symbol | SMAD4 |
|---|---|
| Entrez Gene | 4089 |
| Alt Symbol | DPC4, JIP, MADH4, MYHRS |
| Species | Human |
| Gene Type | protein-coding |
| Description | SMAD family member 4 |
| Other Description | MAD homolog 4|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|deletion target in pancreatic carcinoma 4|mothers against decapentaplegic homolog 4|mothers against decapentaplegic, Drosophila, homolog of, 4 |
| Swissprots | A8K405 Q13485 |
| Accessions | AAC03051 AHA34184 AHA34185 AHA34186 BAB40977 EAW62985 EAW62986 EAW62987 Q13485 AK290770 BAF83459 AU120224 BC002379 AAH02379 BM701399 BX647129 DQ891840 ABM82766 DQ895028 ABM85954 U44378 AAA91041 NM_005359 NP_005350 |
| Function | In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD |
| Subcellular Location | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD. PDPK1 prevents its nuclear translocation in response to TGF- beta. |
| Top Pathways | Wnt signaling pathway, Pancreatic cancer, HTLV-I infection, Colorectal cancer, Chronic myeloid leukemia |
Smad4 CRISPR/Cas9 KO Plasmid (h) - sc-400110 from Santa Cruz Biotechnology
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Smad4 HDR Plasmid (h) - sc-400110-HDR from Santa Cruz Biotechnology
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Smad4 Double Nickase Plasmid (h) - sc-400110-NIC from Santa Cruz Biotechnology
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Smad4 Double Nickase Plasmid (h2) - sc-400110-NIC-2 from Santa Cruz Biotechnology
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Smad4 CRISPR Activation Plasmid (h) - sc-400110-ACT from Santa Cruz Biotechnology
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Smad4 CRISPR Activation Plasmid (h2) - sc-400110-ACT-2 from Santa Cruz Biotechnology
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Smad4 Lentiviral Activation Particles (h) - sc-400110-LAC from Santa Cruz Biotechnology
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Smad4 Lentiviral Activation Particles (h2) - sc-400110-LAC-2 from Santa Cruz Biotechnology
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Smad4 siRNA (h) - sc-29484 from Santa Cruz Biotechnology
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Smad4 shRNA Plasmid (h) - sc-29484-SH from Santa Cruz Biotechnology
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Smad4 shRNA (h) Lentiviral Particles - sc-29484-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU018671 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1070 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1070 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1309 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR00028 from Sigma-Aldrich
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SMAD4 siRNA (Human) - CRH2772 from Cohesion Biosciences
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Smad4 RNAi - H00004089-R01 from Novus Biologicals
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SMAD4 - MBS8238803 from MyBioSource
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shRNA set against Human SMAD4 (NM_005359.5) - SHH412984 from Creative biogene
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SMAD4 shRNA Plasmid (Human) - CHH2772 from Cohesion Biosciences
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