G3BP1

Gene Symbol G3BP1
Entrez Gene 10146
Alt Symbol G3BP, HDH-VIII
Species Human
Gene Type protein-coding
Description GTPase activating protein (SH3 domain) binding protein 1
Other Description ATP-dependent DNA helicase VIII|G3BP-1|GAP SH3 domain-binding protein 1|GAP binding protein|Ras-GTPase-activating protein SH3-domain-binding protein|RasGAP-associated endoribonuclease G3BP|ras GTPase-activating protein-binding protein 1
Swissprots Q13283 Q5HYE9
Accessions CAH05301 EAW61659 EAW61660 EAW61661 EAW61662 Q13283 AK091592 AK128316 AK130003 BAC85272 AK222606 BAD96326 AK300098 BAG61899 AK303580 BAH13990 AK309172 AK311635 AK312572 BAG35467 BC006997 AAH06997 BC108278 AAI08279 BG703413 BT019384 AAV38191 BU517281 BX647869 CAI46065 BX648012 CAI46067 CR536535 CAG38772 DQ893058 ABM83984 DQ896308 ABM87307 U32519 AAB07787 X78262 XM_006714749 XP_006714812 XM_006714750 XP_006714813 NM_005754 NP_005745 NM_198395 NP_938405
Function May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. {ECO:0000269|PubMed:11604510, ECO:0000269|PubMed:9889278}.
Subcellular Location Cytoplasm. Cytoplasm, cytosol. Cytoplasmic granule. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS. {ECO:0000250}.
Tissue Specificity Ubiquitous.

G3BP1 (H-10) - sc-365338 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
G3BP1 (H-10): sc-365338. Western blot analysis of G3BP1 expression in Ramos (A) and K-562 (B) whole cell lysates.

G3BP1 (C-14) - sc-70283 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
G3BP1 (C-14): sc-70283. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear staining of cells in glomeruli and cytoplasmic and nuclear staining of cells in tubules.

G3BP1 (P-18) - sc-70286 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
G3BP1 (P-18): sc-70286. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in glomeruli and cytoplasmic staining of cells in tubules.

G3BP1 (H-94) - sc-98561 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
G3BP1 (H-94): sc-98561. Immunoperoxidase staining of formalin fixed, paraffin-embedded uterine cervix tissue showing cytoplasmic and nuclear staining of squamous epithelial cells.

G3BP1 (TT-Y) - sc-81940 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
G3BP1 (TT-Y): sc-81940. Western blot analysis of G3BP1 expression in A-431 whole cell lysate.

p-G3BP1 (Ser 232) - sc-101684 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated G3BP1 expression in 293 whole cell lysate (A,B). Blots were probed with p-G3BP1 (Ser 232): sc-101684 preincubated with cognate phosphorylated peptide (A) and p-G3BP1 (Ser 232): sc-101684 (B).

Anti-G3BP (phospho S232) antibody - ab38463 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab38463 at a 1:50 dilution staining G3BP in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue. Left image without phosphopeptide. Right image with phosphopeptide.

Anti-G3BP (phospho S232) antibody - ab131243 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-G3BP (phospho S232) antibody (ab131243) at 1/500 dilutionLane 1 : Untreated 293 cell extractLane 2 : Extract from 293 cells treated with starvation

Anti-G3BP (phospho S232) antibody - ab194925 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-G3BP (phospho S232) antibody (ab194925) at 1/500 dilutionLane 1 : Untreated 293 cell extractLane 2 : Starvation treated 293 cell extract

Anti-G3BP (phospho S232) antibody - ab192186 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-G3BP (phospho S232) antibody (ab192186) at 1/500 dilutionLane 1 : 293 whole cell lysate with antigen-specific peptideLane 2 : 293 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-G3BP antibody - ab56574 from Abcam

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ab56574 staining G3BP in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.Top row - untreated cells. Bottom row - cells treated with sodium arsenite. Left - G3BP, Middle - Nucleus, Right - Merge.Stress granules are visible in cells treated with sodium arsenite, whereas G3BP is dispersed in the cytoplasm in untreated cells.See Abreview

Anti-G3BP antibody - ab39533 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab39533 diluted 1:50, with (right) or without (left) pre-incubation with synthetic peptide.

Anti-G3BP antibody - ab123379 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-G3BP antibody (ab123379) at 1/500 dilutionLane 1 : 293 cell extract with blocking peptideLane 2 : 293 cell extract

Anti-G3BP antibody - ab48739 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-G3BP antibody (ab48739) at 2.5 µg/mlLane 1 : HepG2 cell lysateLane 2 : HepG2 cell lysate with blocking peptide at 2 µg/mlLysates/proteins at 25 µg per lane.

Anti-G3BP antibody - ab116365 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-G3BP antibody (ab116365) at 1/100 dilution + Jurkat cell line lysate at 35 µg

Anti-G3BP antibody [2F3] - ab117076 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2F3
All lanes : Anti-G3BP antibody [2F3] (ab117076)Lane 1 : G3BP transfected 293T cell line lysateLane 2 : non-transfected 293T cell line lysate

Anti-G3BP antibody [EPR13985(B)] - ab181149 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13985(B)
All lanes : Anti-G3BP antibody [EPR13985(B)] (ab181149) at 1/20000 dilutionLane 1 : Hela cell lysate with NFDM/TBSTLane 2 : Jurkat cell lysate with NFDM/TBSTLane 3 : Ramos cell lysate with NFDM/TBSTLane 4 : 293 cell lysate with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryPeroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Anti-G3BP antibody [EPR13986(B)] - ab181150 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13986(B)
All lanes : Anti-G3BP antibody [EPR13986(B)] (ab181150) at 1/5000 dilutionLane 1 : Jurkat cell lysate with NFDM/TBSTLane 2 : Ramos cell lysate with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryPeroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Anti-G3BP1 / G3BP Antibody (phospho-Ser232) - LS-C123588 from LifeSpan Bioscience

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-G3BP1 / G3BP Antibody - LS-C123589 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-G3BP1 / G3BP Antibody (C-Terminus) - LS-C125636 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-G3BP1 / G3BP Antibody (phospho-Ser232) - LS-C190818 from LifeSpan Bioscience

Prices $485.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-G3BP1 / G3BP Antibody (aa400-430, AP) - LS-C222582 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-G3BP1 / G3BP Antibody (aa400-430, APC) - LS-C222583 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-G3BP1 / G3BP Antibody (aa400-430, Biotin) - LS-C222584 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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