IRS2

Gene Symbol IRS2
Entrez Gene 8660
Alt Symbol IRS-2
Species Human
Gene Type protein-coding
Description insulin receptor substrate 2
Other Description -
Swissprots Q9BZG0 Q96RR2 Q9Y6I5 Q9Y4H2
Accessions AAD55239 AAG50013 AAK66750 AAK66751 AAK83053 BAA24500 BAB84688 CCQ43232 Q9Y4H2 AF073310 AAD21531 AF161416 AAF28976 BC012414 BC026044 BC042858 BC063516 BC066356 BC110638 CA416129 CA952668 NM_003749 NP_003740
Function May mediate the control of various cellular processes by insulin.
Subcellular Location Cytoplasm, cytosol {ECO:0000250}.
Top Pathways Insulin signaling pathway, Adipocytokine signaling pathway, Type II diabetes mellitus, MicroRNAs in cancer, Non-alcoholic fatty liver disease (NAFLD)

IRS-2 Antibody - 4502 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from CHO IR/4PS cells (transfected with insulin receptor and IRS-2) or CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), showing no change in total protein levels with insulin stimulation (100 nM for 5 min), using IRS-2 Antibody (top) or IRS-1 Antibody #2382 (bottom).

IRS-2 (L1326) Antibody - 3089 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of H-4-II-E and HeLa cells using IRS-2 (L1326) Antibody.

IRS-2 (B-5) - sc-390761 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRS-2 (B-5): sc-390761. Western blot analysis of IRS-2 expression in HeLa whole cell lysate.

IRS-2 (H-205) - sc-8299 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
IRS-2 (H-205): sc-8299. Western blot analysis of IRS-2 expression in HeLa (A) and 3T3-L1 (B) whole cell lysates.

IRS-2 (M-19) - sc-1555 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
IRS-2 (M-19): sc-1555. Immunofluorescence staining of methanol-fixed 3T3-L1 cells showing cytoplasmic staining.

Anti-IRS2 (phospho S731) antibody - ab3690 from Abcam

Prices $390.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
IP: 3T3 cell lysate immunoprecipitated with Anti-IRS-2 (pSER731) (ab3690)

Anti-IRS2 antibody - ab84906 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84906, at a 1/100 dilution, staining IRS2 in formalin fixed, paraffin embedded human testicular seminoma tissue by Immunohistochemistry, using DAB staining.

Anti-IRS2 antibody [EP903Y] - ab46811 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP903Y
Anti-IRS2 antibody [EP903Y] (ab46811) at 1/100000 dilution + 293 cell lysate at 10ug/lane, plus insulin SecondaryGoat-anti rabbit, HRP labelled, at 1/2000 dilution

Anti-IRS2 antibody [EP976Y] - ab52606 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP976Y
ab52606 stained SKNSH cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52606 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-IRS2 antibody [EPR1650(2)] - ab133276 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1650(2)
All lanes : Anti-IRS2 antibody [EPR1650(2)] (ab133276) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : 293T+insulin cell lysateSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-IRS2 antibody [EPR904(2)] - ab134101 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR904(2)
Overlay histogram showing Hela cells stained with ab134101 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134101, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-IRS2 / IRS-2 Antibody (phospho-Ser731) - LS-C8287 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (Ser731) - LS-C33930 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (phospho-Ser731) - LS-C33929 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (phospho-Ser731) - LS-C189059 from LifeSpan Bioscience

Prices $395.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (Tyr978, AP) - LS-C278553 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (Tyr978, APC) - LS-C278554 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (phospho-Tyr978) - LS-C97116 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Dot blot of anti-Phospho-IRS2-pY978 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Anti-IRS2 / IRS-2 Antibody (Tyr978, Biotin) - LS-C278555 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (Tyr978, Azide-free, HRP) - LS-C278556 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (Tyr978, FITC) - LS-C278557 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (Tyr978, PE) - LS-C278558 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody (aa1100-1150) - LS-C288228 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-IRS2 / IRS-2 Antibody - LS-C62152 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-IRS2 / IRS-2 Antibody (phospho-Tyr978) - LS-C330489 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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