CD3E

Gene Symbol CD3E
Entrez Gene 916
Alt Symbol IMD18, T3E, TCRE
Species Human
Gene Type protein-coding
Description CD3e molecule, epsilon (CD3-TCR complex)
Other Description CD3-epsilon|CD3e antigen, epsilon polypeptide (TiT3 complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain|T-cell surface glycoprotein CD3 epsilon chain
Swissprots A8K997 P07766
Accessions AAA52295 AAA61062 BAJ16130 EAW67360 EAW67361 EAW67362 EAW67363 EAW67364 P07766 AK292612 BAF85301 AK301255 BAG62822 AK311641 BC049847 AAH49847 BI910359 BP366535 X03884 CAA27516 NM_000733 NP_000724
Function The CD3 complex mediates signal transduction.
Subcellular Location Membrane; Single-pass type I membrane protein.
Top Pathways Hematopoietic cell lineage, T cell receptor signaling pathway, Primary immunodeficiency, Chagas disease (American trypanosomiasis), HTLV-I infection

CD3ε (CD3-12) Rat mAb - 4443 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rat
Clonality Monoclonal
Clone CD3-12
Western blot analysis of total cell lysates from DND41 and Molt4 cells using CD3ε (CD3-12) Rat mAb.

CD3 (PC3/188A) - sc-20047 from Santa Cruz Biotechnology

CD3 (PC3/188A) Alexa Fluor 488: sc-20047 AF488. Intracellular FCM analysis of fixed and permeabilized human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgG1: sc-3890.

CD3-ε (A-1) - sc-137095 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD3-ε (A-1): sc-137095. Western blot analysis of CD3-ε expression in non-transfected 293T: sc-117752 (A), human CD3-ε transfected 293T: sc-116055 (B), Jurkat (C) and CCRF-CEM (D) whole cell lysates.

CD3-ε (H-12) - sc-137234 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD3-ε (H-12): sc-137234. Western blot analysis of CD3-ε expression in Jurkat (A) and CCRF-CEM (B) whole cell lysates.

CD3-ε (G-5) - sc-137096 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD3-ε (G-5): sc-137096. Western blot analysis of CD3-ε expression in non-transfected 293T: sc-117752 (A), human CD3-ε transfected 293T: sc-116055 (B), Jurkat (C) and CCRF-CEM (D) whole cell lysates.

CD3-ε (FL-207) - sc-20918 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CD3- (FL-207): sc-20918. Western blot analysis of CD3- expression in HuT 78 (A) and H9 (B) whole cell lysates.

CD3-ε (M-20) - sc-1127 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CD3-ε (M-20): sc-1127. Western blot analysis of CD3-ε expression in Jurkat whole cell lysate.

CD3-ε (UCH-T1) - sc-1179 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CD3-ε (UCH-T1): sc-1179. Western blot analysis of CD3-ε expression in Jurkat whole cell lysate.

CD3-ε (E-13) - sc-26249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CD3 + CD44 antibody [M2AB, 7F1] (FITC) - ab52305 from Abcam

Prices $165.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone M2AB, 7F1

Anti-CD3 epsilon antibody - ab185811 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185811. Blue DAPI for nuclear staining.

Anti-CD3 epsilon antibody - ab119332 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of CD3-epsilon staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119332, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD3 epsilon antibody - ab186134 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CD3 epsilon antibody (ab186134) at 0.1 µg/mlLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-CD3 epsilon antibody [0.N.47] - ab33524 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 0.N.47

Anti-CD3 epsilon antibody [APA1/1] - ab134408 from Abcam

Prices $373.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone APA1/1

Anti-CD3 epsilon antibody [APA1/1] (Phycoerythrin) - ab91498 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone APA1/1

Anti-CD3 epsilon antibody [APA11] (Alexa Fluor® 488) - ab187572 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone APA11

Anti-CD3 epsilon antibody [E272] - ab32186 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E272
Western blot analysis of immunoprecipitation pellets from Jurkat cell lysate or PBS (lane 2: negative control), using ab32186 at a 1/50 dilution. The predicted band size of 23 kDa was observed.

Anti-CD3 epsilon antibody [E292] - ab32327 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E292
Anti-CD3 epsilon antibody [E292] (ab32327) at 1/5000 dilution + Jurkat cell lysate

Anti-CD3 epsilon antibody [EP449E] - ab52959 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP449E
Human peripheral blood lymphocytes stained with ab52959 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab52959, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blo

Anti-CD3 epsilon antibody [EPR5361(2)] - ab133628 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5361(2)
All lanes : Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : Human thymus cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-CD3 epsilon antibody [mAbcam97707] - ab97707 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam97707
All lanes : Anti-CD3 epsilon antibody [mAbcam97707] (ab97707) at 5 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-CD3 epsilon antibody [MM0167-3F43] - ab89205 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone MM0167-3F43
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CD3 with ab89205. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

Anti-CD3 epsilon antibody [PS1], prediluted - ab74705 from Abcam

Prices $376.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone PS1
Formalin-fixed, paraffin-embedded human tonsil stained with ab74705 using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of T cells.

Anti-CD3 epsilon antibody [UCHT1] (Biotin) - ab25611 from Abcam

Prices $373.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone UCHT1
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