HIST4H4

Gene Symbol HIST4H4
Entrez Gene 121504
Alt Symbol H4/p
Species Human
Gene Type protein-coding
Description histone cluster 4, H4
Other Description histone 4, H4|histone H4
Accessions AAN01438 AAT68253 EAW96325 AK311916 BAG34857 BC020884 NM_175054 NP_778224
Top Pathways Systemic lupus erythematosus, Viral carcinogenesis, Alcoholism

Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody - ab14723 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody (ab14723) at 1/1000 dilutionLane 1 : YDH8 cka 2-8 ts grown at 30 degrees CLane 2 : YDH8 cka 2-8 ts grown at 38 degrees CSecondaryHRP conjugated anti-Rabbit

Anti-Histone H4 (acetyl K12) antibody [EPR17906] - ab177793 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17906
All lanes : Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793) at 1/10000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysatesLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) untreated whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H4 (acetyl K12) antibody - ChIP Grade - ab46983 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Staining of HeLa cells with ab46983 at a dilution of 1/500.

Anti-Histone H4 (acetyl K16) antibody [EPR1004] - ab109463 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1004
All lanes : Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463) at 1/1000 dilution (unpurified)Lane 1 : HeLa cell lysates, untreated Lane 2 : HeLa cell lysates treated with TSALysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution

Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] - ab177790 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16606
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with ab177790 at 1/5000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Nuclear staining on HeLa cell line is observed. The expression increased after treatment with TSA (50 μg/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:1. ab177790 at 1/15000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. 

Anti-Histone H4 (acetyl K5) antibody - ab114146 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Histone H4 (acetyl K5) antibody (ab114146) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H4 (acetyl K5) antibody - ab124636 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab124636, at 5µg/mL, staining Human prostate by immunohistochemistry of paraffin embedded tissues (IHC-P).

Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade - ab51997 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1000Y
IHC image of ab51997 staining Histone H4 (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51997, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H4 (acetyl K8) antibody - ab61237 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H4 (acetyl K8) antibody (ab61237) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of acetylated peptide.

Anti-Histone H4 (acetyl K8) antibody - ab51827 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H4 (acetyl K8) antibody (ab51827) at 1/1000 dilutionLane 1 : Acid extract of HeLa cells, no treatmentLane 2 : Acid extract of HeLa cells, cells treated with sodium butyrateLysates/proteins at 10 µg per lane.

Anti-Histone H4 (acetyl K8) antibody [EP1002Y] - ChIP Grade - ab45166 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1002Y
IHC image of ab45166 staining Histone H4 (acetyl K8) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45166, 1/200, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H4 (asymmetric di methyl R3) antibody - ab194683 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemical analysis of 293T cells labeling Histone H4 (asymmetric di methyl R3) with ab194683 at 1/50 dilution (red) and DAPI (blue)

Anti-Histone H4 (asymmetric di methyl R3) antibody - ab129231 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All batches of ab129231 are tested in Peptide Array against peptides to different Histone H4 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - asymmetric di methyl R3 peptide (ab154780), indicating that this antibody specifically recognises the Histone H4 - asymmetric di methyl R3 modification.ab154780 - Histone H4 - asymmetric di methyl R3ab14791 - Histone H4 - symmetric di methyl R3ab17770 - Histone H4 - mono methyl R3ab154467 - Histone H4 - unmodified

Anti-Histone H4 (Biotinylated K5) antibody - ab17343 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H4 (Biotinylated K5) antibody (ab17343) at 1 µg/mlLane 1 : HeLa nuclear extractLane 2 : HeLa nuclear extract with Human Histone H4 (biotinylated K5) peptide (ab17632) at 1 µg/mlLane 3 : HeLa nuclear extract with Human Histone H4 peptide (ab15825) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Histone H4 (citrulline R3) antibody - ab81797 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ELISA control of the specificity of ab81797 using H4-3cit peptide, H4-Arg3 peptide and H3-cit2, 8, 17 peptide.

Anti-Histone H4 (crotonyl K5) antibody [EPR17903] - ab177844 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17903
All lanes : Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844) at 1/1000 dilutionLane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H4 (di methyl K20) antibody - ab118513 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
ab118513 staining Histone H4 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab118513 at 1µg/ml and ab6046 (anti beta Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Rabbit secondary (ab150084) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

Anti-Histone H4 (di methyl K20) antibody - ab51878 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
IHC image of Histone (di methyl K20) staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51878, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H4 (di methyl K20) antibody - ChIP Grade - ab9052 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H4 (di methyl K20) antibody - ChIP Grade (ab9052) at 1/2000 dilutionLane 1 : yH4 17-24 peptideLane 2 : Unmodified yH4 17-24 peptideLane 3 : Mono methyl K20 yH4 17-24 peptideLane 4 : Di methyl K20 yH4 17-24 peptideLane 5 : Tri methyl K20 yH4 17-24 peptideBlocking peptides at 1 µg/ml per lane.Performed under reducing conditions.Steve Sanders, Tony Kouzarides lab, University of Cambridge

Anti-Histone H4 (di methyl K79) antibody - ab2885 from Abcam

Prices $380.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H4 (di methyl K79) antibody (ab2885) at 1/500 dilutionLane 1 : Calf Thymus histone prepLane 2 : Calf Thymus histone prep with Human Histone H3 (mono methyl K79) peptide (ab4555) at 0.1 µgLane 3 : Calf Thymus histone prep with Human Histone H3 (di methyl K79) peptide (ab4556) at 0.1 µgLane 4 : Calf Thymus histone prep with Human Histone H3 (tri methyl K79) peptide (ab4557) at 0.1 µgLane 5 : Calf Thymus histone prep with Human Histone H4 (di methyl K79) peptide (ab4560) at 0.1 µgLane 6 : Calf Thymus histone prep with Human Histone H3 (di methyl K27) peptide (ab1781) at 0.1 µgLysates/proteins at 1 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilutionRabbit polyclonal to Histone H4 Di Methyl K79 at 1/500 on Calf Thymus histone prep (1 ug per lane).Lane 1: ab2885 1/500Lane 2: ab2885 1/500 H3 Mono Me K79 peptide (ab4555), 0.1 ugLane 3: ab2885 1/500 H3 Di Me K79 peptide (ab4556), 0.1 ugLane 4: ab2885 1/500 H3 Tri Me K79 peptide  (ab4557), 0.1

Anti-Histone H4 (di methyl R3) antibody - ab194696 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H4 (di methyl R3) antibody (ab194696) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : Histone H4 protein expressed in E.coli

Anti-Histone H4 (formyl K12) antibody - ab177068 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab177068 staining Histone H4 (formyl K12) in HeLa cells. The cells were fixed with 100% methanol (5min) and then permeabilised using 0.1% PBS-Triton. Cells were then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab177068 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 1/1000 dilution (shown in green). AlexaFluor®594 Mouse monoclonal [DM1A] to alpha Tubulin (ab195889) - Microtubule Marker was used at a 1/200 dilution and incubated for 1h with the cells, to label Microtubules (shown in Red). The nuclear counter stain is DAPI (blue), which was added to the secondary antibody mixture.

Anti-Histone H4 (mono methyl K16) antibody [EPR17910] - ab177849 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17910
All lanes : Anti-Histone H4 (mono methyl K16) antibody [EPR17910] (ab177849) at 1/2000 dilutionLane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H4 (mono methyl K20) antibody [EPR16999] - ChIP Grade - ab177188 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16999
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab177188 (blue), and 20μl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach). Primers are located in the first kb of the transcribed region.

Anti-Histone H4 (mono methyl K20) antibody [mAbcam 16974] - ab16974 from Abcam

Prices $376.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 16974
ELISA using ab16974 at antibody concentrations between 1/100 and 1/10000.The orange line indicates binding to the Histone H4 peptide - mono methyl K20 (ab17043). Binding to the following peptides was either not seen or was very weak:Histone H4 peptide - tri methyl K20 (ab17567)  - green line,Histone H4 peptide - di methyl K20 (ab14964)  - blue line,Histone H4 peptide - unmodified (ab2622) - red line.This indicates the specificity of ab16794 for mono methyl K20 of Histone H4.
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