His3.3B

Gene Symbol His3.3B
Entrez Gene 31848
Alt Symbol CG8989, Dmel\CG8989, H3.3, H3.3B, His3.3, HisH3-3Q, PH3, anon-EST:fe1B6, dH3.3B
Species Drosophila
Gene Type protein-coding
Description Histone H3.3B
Other Description CG8989-PA|CG8989-PB|CG8989-PC|CG8989-PE|His3.3B-PA|His3.3B-PB|His3.3B-PC|His3.3B-PE|HistoneH3|anon-fast-evolving-1B6|histone H3-3Q|histone H3.3|histone H3.3B|histone variant 3.3|histone variant H3.3
Accessions AAF46452 AAF52213 AAN09245 AAN10526 AAO41645 AGB92618 AGB95217 CAA57077 CAA57080 CAR80790 CBX84261 P84249 S20109 S61215 AY071057 AAL48679 AY118325 AAM48354 AY119629 AAM50283 X53822 CAA37819 X82257 CAA57712 NM_001272445 NP_001259374 NM_078540 NP_511095 NM_167180 NP_727314 NM_176719 NP_788892

Anti-Histone H3 (phospho S10) antibody [E173] - ab32107 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E173
All lanes : Anti-Histone H3 (phospho S10) antibody [E173] (ab32107) at 1/2500 dilutionLane 1 : Hela cell lysate, untreated.Lane 2 : Hela cell lysate, treated with Alkaline Phosphatase.

Anti-Histone H3 (phospho S28) antibody - ab5169 from Abcam

Prices $401.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 (phospho S28) antibody (ab5169) at 1 µg/ml + Hela Whole Cell Lysate - Colcemid Treated at 2.5 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 antibody - ab134198 from Abcam

Prices $376.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
All lanes : Anti-Histone H3 antibody (ab134198) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control and ChIP Grade - ab176842 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16987
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Histone H3 with ab176842 at 1/2000 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 Goat secondary antibody) at 1/500 dilution (red).The negative controls are as follows;-ve control 1: ab176842 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. 

Anti-Histone H3 antibody [mAbcam 10799] - ChIP Grade - ab10799 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 10799
Histone H3 [mAbcam 10799] - ChIP Grade was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Mouse monoclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10799.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 15kDa; [mAbcam 10799] Histone H3 - ChIP Grade

Anti-Histone H3 antibody - Nuclear Loading Control (HRP) - ab21054 from Abcam

Prices $382.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of Histone H3 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21054, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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