LDHA

Gene Symbol LDHA
Entrez Gene 3939
Alt Symbol GSD11, HEL-S-133P, LDH1, LDHM, PIG19
Species Human
Gene Type protein-coding
Description lactate dehydrogenase A
Other Description L-lactate dehydrogenase A chain|LDH muscle subunit|LDH-A|LDH-M|cell proliferation-inducing gene 19 protein|epididymis secretory sperm binding protein Li 133P|lactate dehydrogenase M|proliferation-inducing gene 19|renal carcinoma antigen NY-REN-59
Swissprots P00338 Q6ZNV1 B7Z5E3 Q6IBM7 Q9UDE8 F8W819 B4DKQ2 Q53G53 Q9UDE9 D3DQY3
Accessions CAA26879 EAW68395 EAW68396 EAW68397 P00338 AK026515 AK130035 AK130105 AK130587 BAC85389 AK223078 BAD96798 AK296089 BAG58843 AK296667 BAG59264 AK298834 BAH12879 AK307477 AY423727 AAS00490 BC067223 AAH67223 BG424022 BG760757 BM995301 BU507758 CD243986 CR456775 CAG33056 CR541714 CAG46515 DA609380 DB502157 DC392310 DC396757 EU794632 ACJ13686 X02152 CAA26088 NM_001135239 NP_001128711 NM_001165414 NP_001158886 NM_001165415 NP_001158887 NM_001165416 NP_001158888 NM_005566 NP_005557 NR_028500
Subcellular Location Cytoplasm.
Top Pathways Glycolysis / Gluconeogenesis, Cysteine and methionine metabolism, Central carbon metabolism in cancer, Glucagon signaling pathway, Pyruvate metabolism

Phospho-LDHA (Tyr10) Antibody - 8176 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Phospho-LDHA (Tyr10) Antibody.

LDHA/LDHC (C28H7) Rabbit mAb - 3558 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C28H7
Western blot analysis of extracts from various cell types using LDHA/LDHC (C28H7) Rabbit mAb.

LDHA (C4B5) Rabbit mAb - 3582 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C4B5
Western blot analysis of extracts from various cell types using LDHA (C4B5) Rabbit mAb.

LDHA Antibody - 2012 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using LDHA Antibody.

LDH (H-160) - sc-33781 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LDH (H-160): sc-33781. Immuno?fluorescence staining of methanol-fixed HeLa cells showing cytoplasmic local?ization.

LDH (S-12) - sc-27231 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

LDH (V-17) - sc-27232 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LDH (V-17): sc-27232. Western blot analysis of LDH expression in LADMAC (A), SK-N-SH (B), SJRH30 (C), F9 (D) and HS 181.Tes (E) whole cell lysates.

LDH-A (5) - sc-130327 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LDH-A (5): sc-130327. Western blot analysis of human recombinant LDH-A.

LDH-A (E-9) - sc-137243 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LDH-A (E-9): sc-137243. Western blot analysis of LDH-A expression in HeLa (A), Y79 (B), SJRH30 (C) and SK-N-SH (D) whole cell lysates.

LDH-A (F-3) - sc-137244 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LDH-A (F-3): sc-137244. Western blot analysis of LDH-A expression in HeLa (A), Y79 (B), SJRH30 (C) and SK-N-SH (D) whole cell lysates.

LDH-A (N-14) - sc-27230 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LDH-A (N-14): sc-27230. Western blot analysis of LDH-A expression in HeLa (A) and ES-2 (B) whole cell lysates and rat skeletal muscle tissue extract (C).

Anti-Lactate Dehydrogenase antibody - ab2101 from Abcam

Host Goat
Clonality Polyclonal
ab2101 staining Lactate Dehydrogenase in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Rats were anesthetized and intracardially perfused with 500 ml of normal saline at room temperature, followed by 500 ml of ice-cold, freshly made 4% paraformaldehyde in phosphate buffer (PB, 0.1 M, pH 7.4). Alexa Fluor® 546 conjugated anti goat antibody was used as secondary.

Anti-Lactate Dehydrogenase antibody - ab191332 from Abcam

Prices $384.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
All lanes : Anti-Lactate Dehydrogenase antibody (ab191332) at 1/400 dilutionLane 1 : HeLa cell extract cytoplasmic fractionLane 2 : HeLa cell extract cytoplasmic fractionLane 3 : HeLa cell extract cytoplasmic fractionLane 4 : HeLa cell extract cytoplasmic fractionLane 5 : HeLa cell extract nuclear fractionLane 6 : HeLa cell extract nuclear fractionLane 7 : HeLa cell extract nuclear fractionLane 8 : HeLa cell extract nuclear fractionLysates/proteins at 30 µg per lane.SecondaryIRDye800™ secondary antibody at 1/10000 dilution

Anti-Lactate Dehydrogenase antibody - ab55433 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab55433 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55433, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Lactate Dehydrogenase antibody - ab71494 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-Lactate Dehydrogenase antibody - ab103804 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Lactate Dehydrogenase antibody (ab103804) at 5 µg/ml + Human kidney tissue lysate at 50 µg

Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] - ab135396 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 98A-1F9BB1
All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/mlLane 1 : LadderLane 2 : Human liver homogenate at 15 µgLane 3 : Human heart homogenate at 15 µgLane 4 : Rat liver homogenate at 15 µgLane 5 : Rat heart homogenate at 15 µgLane 6 : Mouse liver homogenate at 15 µgLane 7 : Mouse heart homogenate at 15 µgLane 8 : LadderLane 9 : Human fibroblast whole cell lysate at 15 µgLane 10 : HeLa whole Cell Lysate at 15 µgLane 11 : HepG2 whole cell lysate at 15 µgLane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µgLane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µgLane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µgLane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µgSecondaryGoat polyclonal to Mouse IgG - AP at 1/3000 dilution

Anti-Lactate Dehydrogenase antibody [AF14A11] - ab85326 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AF14A11
All lanes : Anti-Lactate Dehydrogenase antibody [AF14A11] (ab85326) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLane 5 : SK-N-MC cell lysateLane 6 : RD cell lysateLane 7 : IMR32 cell lysateObserved band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-Lactate Dehydrogenase antibody [AF9D1] - ab85325 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AF9D1
All lanes : Anti-Lactate Dehydrogenase antibody [AF9D1] (ab85325) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : 293T cell lysateLane 4 : SK-N-MC cell lysateLane 5 : RD cell lysateLane 6 : PC12 cell lysateLane 7 : NIH3T3 cell lysateObserved band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-Lactate Dehydrogenase antibody (Biotin) - ab7638 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-Lactate Dehydrogenase antibody [EP1563Y] - ab134187 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1563Y
Anti-Lactate Dehydrogenase antibody [EP1563Y] (ab134187) at 1/1000 dilution + A549 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Lactate Dehydrogenase antibody [EP1566Y] - ab52488 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1566Y
Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488) at 1/100000 dilution + Hela cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Anti-Lactate Dehydrogenase Isoenzyme V antibody - ab9002 from Abcam

Prices $384.00
Sizes 500 µl
Host Sheep
Clonality Polyclonal
All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002) at 1 µg/mlLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryRabbit polyclonal Secondary Antibody to Sheep IgG - H&L (HRP) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Lactate Dehydrogenase Isoenzyme V antibody - ab85472 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 6 : Cervix (Human) Whole Cell Lysate - adult normal tissue (ab29226)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Lactate Dehydrogenase Isoenzyme V antibody [EPR1564] - ab101562 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1564
All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody [EPR1564] (ab101562) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : A375 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.
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