EZH2

Gene Symbol EZH2
Entrez Gene 2146
Alt Symbol ENX-1, ENX1, EZH1, EZH2b, KMT6, KMT6A, WVS, WVS2
Species Human
Gene Type protein-coding
Description enhancer of zeste 2 polycomb repressive complex 2 subunit
Other Description enhancer of zeste homolog 2|histone-lysine N-methyltransferase EZH2|lysine N-methyltransferase 6
Swissprots B7Z7L6 B3KS30 Q92857 B2RAQ1 B7Z1D6 Q15755 Q75MG3 Q15910 Q96FI6
Accessions AAC50591 AAS02035 AAS02036 AAS07448 AEN79481 CAA03482 CCO13726 EAL24423 EAL24424 EAW80067 EAW80068 EAW80069 EAW80070 Q15910 AB208895 BAD92132 AK092676 BAG52592 AK293239 BAH11472 AK302216 BAH13652 AK303585 BAH13991 AK303637 BAH14002 AK314291 BAG36948 AY519465 AAS09975 BC010858 AAH10858 BG829016 BM974565 BU953788 CN283479 DQ894179 ABM85105 JF327843 AEA07595 U61145 AAC51520 X95653 CAA64955 XM_005249962 XP_005250019 XM_005249963 XP_005250020 XM_005249964 XP_005250021 XM_011515883 XP_011514185 XM_011515884 XP_011514186 XM_011515885 XP_011514187 XM_011515886 XP_011514188 XM_011515887 XP_011514189 XM_011515888 XP_011514190 XM_011515889 XP_011514191 XM_011515890 XP_011514192 XM_011515891 XP_011514193 XM_011515892 XP_011514194 XM_011515893 XP_011514195 XM_011515894 XP_011514196 XM_011515895 XP_011514197 XM_011515896 XP_011514198 XM_011515897 XP_011514199 XM_011515898 XP_011514200 XM_011515899 XP_011514201 XM_011515900 XP_011514202 XM_011515901 XP_011514203 XM_011515902 XP_011514204 NM_001203247 NP_001190176 NM_001203248 NP_001190177 NM_001203249 NP_001190178 NM_004456 NP_004447 NM_152998 NP_694543
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian gen
Subcellular Location Nucleus {ECO:0000269|PubMed:12101246, ECO:0000269|PubMed:14532106, ECO:0000269|PubMed:15231737, ECO:0000269|PubMed:9584199}.
Tissue Specificity Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. {ECO:0000269|PubMed:14532106}.
Top Pathways MicroRNAs in cancer

Ezh2 (D2C9) XP ® Rabbit mAb (Biotinylated) - 12408 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2C9
Western blot analysis of extracts from MCF7 and COS-7 cells using Ezh2 (D2C9) XP ® Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for western detection.

Ezh2 (D2C9) XP ® Rabbit mAb - 5246 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2C9
Western blot analysis of extracts from MCF7, Neuro-2a, and COS-7 cell lines using Ezh2 (D2C9) XP ® Rabbit mAb.

Ezh2 (AC22) Mouse mAb - 3147 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone AC22
Western blot analysis of extracts from various cell lines using Ezh2 (AC22) Mouse mAb.

Ezh2 Antibody - 4905 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Ezh2 Antibody.

ENX-1 (C-1) - sc-166609 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ENX-1 (C-1): sc-166609. Western blot analysis of ENX-1 expression in HeLa (A), HS 181.Tes (B), NTERA-2 cl.D1 (C), JAR (D) and F9 (E) whole cell lysates.

ENX-1 (D-8) - sc-137255 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ENX-1 (D-8): sc-137255. Western blot analysis of ENX-1 expression in non-transfected: sc-117752 (A) and mouse ENX-1 transfected: sc-120053 (B) 293T whole cell lysates.

ENX-1 (N-20) - sc-17268 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ENX-1 (N-20): sc-17268. Western blot analysis of ENX-1 expression in non-transfected: sc-110760 (A) and human ENX-1 transfected: sc-111345 (B) 293 whole cell lysates.

ENX-1 (H-80) - sc-25383 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ENX-1 (H-80): sc-25383. Western blot analysis of ENX-1 expression in non-transfected: sc-110760 (A) and human ENX-1 transfected: sc-111345 (B) 293 whole cell lysates.

ENX-1 (T-20) - sc-17270 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ENX-1 (T-20): sc-17270. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

Anti-KMT6 / EZH2 [EPR9307(2)] antibody - N-terminal - ab191080 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9307(2)
Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100-permeabilized) labeling KMT6 / EZH2 with ab191080 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). 

Anti-KMT6 / EZH2 (phospho S21) antibody - ab84989 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84989 at 1/250 dilution staining KMT6 / EZH2 in serial sections of human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right).

Anti-KMT6 / EZH2 (phospho T487) antibody [EPR1410] - ab109398 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1410
All lanes : Anti-KMT6 / EZH2 (phospho T487) antibody [EPR1410] (ab109398) at 1/1000 dilutionLane 1 : HeLa cell lysatesLane 2 : HeLa cell lysates treated with LPLysates/proteins at 10 µg per lane.

Anti-KMT6 / EZH2 (phospho T487) antibody [EPR1410] (Alexa Fluor® 488) - ab196466 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1410
ab196466 staining KMT6 / EZH2 in HeLa cells. The cells were incubated with 2000 units/ml LP at 31°C for 5 hours (Treated) or solvent-only for control purposes (Non-treated). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196466 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-KMT6 / EZH2 (phospho T487) antibody [EPR1410] (Alexa Fluor® 647) - ab196197 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1410
ab196197 staining KMT6 / EZH2 in HeLa cells. The cells were incubated with 2000 units/ml LP at 31°C for 5 hours (Treated) or solvent-only for control purposes (Non-treated). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196197 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/200 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-KMT6 / EZH2 antibody - ab186006 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µgLanes 2 - 4 : Anti-KMT6 / EZH2 antibody (ab186006) at 1 µg/mlLane 1 : 293T whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : Mouse TCMK-1 cells.lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-KMT6 / EZH2 antibody - ab156269 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence of KMT6 / EZH2 in 293 cells with ab156269 at 20 ug/mL.

Anti-KMT6 / EZH2 antibody - ab124522 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-KMT6 / EZH2 antibody (ab124522) at 4 µg/ml + Rat Kidney tissue lysate

Anti-KMT6 / EZH2 antibody - ab168764 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-KMT6 / EZH2 antibody (ab168764) at 1 µg/mlLane 1 : KMT6 / EZH2 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-KMT6 / EZH2 antibody - ab186022 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of KMT6 / EZH2 in immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186022 at 6 µg/mg lysate for IP. An  alternative anti-KMT6 / EZH2 antibody was used at 1 µg/ml for subsequent western blot detection.Detection: Chemiluminescence with exposure time of 3 minutes.

Anti-KMT6 / EZH2 antibody - ab133793 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin fixed, paraffin-embedded Human placenta tissue labelling KMT6/EZH2 with ab133793 at 5 µg/ml. Primary incubation was followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-KMT6 / EZH2 antibody - ab135084 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-KMT6 / EZH2 antibody (ab135084) at 1/1000 dilution + HeLa total cell extract

Anti-KMT6 / EZH2 antibody [EPR1409(2)] - ab110646 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1409(2)
All lanes : Anti-KMT6 / EZH2 antibody [EPR1409(2)] (ab110646) at 1/1000 dilutionLane 1 : Hela cell lysates Lane 2 : MCF7 cell lysates Lane 3 : K562 cell lysates Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-KMT6 / EZH2 antibody [EPR9307] - ab150433 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9307
All lanes : Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-KMT6 / EZH2 antibody - Aminoterminal end - ab135075 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-KMT6 / EZH2 antibody - Aminoterminal end (ab135075) at 1/1000 dilution + Hela whole cell lysate

Anti-KMT6 / EZH2 antibody - ChIP Grade - ab3748 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-KMT6 / EZH2 antibody - ChIP Grade (ab3748) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Additional bands at : 23 kDa,48 kDa. We are unsure as to the identity of these extra bands.Exposure time : 16 minutes
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