GAB1

Gene Symbol GAB1
Entrez Gene 2549
Alt Symbol -
Species Human
Gene Type protein-coding
Description GRB2-associated binding protein 1
Other Description GRB2-associated binder 1|GRB2-associated-binding protein 1|growth factor receptor bound protein 2-associated protein 1
Swissprots Q13480 A8K152 Q6P1W2 Q4W5G2
Accessions AAY26398 AAY40964 CCH22599 CCH22600 EAX05072 EAX05073 Q13480 AK022142 BAB13970 AK074381 BAB85065 AK289767 BAF82456 AK295684 BAH12155 AK314635 AL049449 BC030115 BC030751 BC064848 AAH64848 DA109618 DA249555 U43885 AAC50380 XM_005262881 XP_005262938 XM_005262882 XP_005262939 XM_006714167 XP_006714230 XM_006714168 XP_006714231 NM_002039 NP_002030 NM_207123 NP_997006
Function Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR).
Top Pathways Renal cell carcinoma, ErbB signaling pathway, Neurotrophin signaling pathway, Bacterial invasion of epithelial cells, Proteoglycans in cancer

Phospho-Gab1 (Tyr307) Antibody - 3234 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HEK293 cells, untreated or treated with In1B for indicated times, using Phospho-Gab1 (Tyr307) Antibody (upper) or Gab1 Antibody #3232 (lower).

Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb - 3233 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C32H2
Western blot analysis of cell extracts from T47D cells, untreated or stimulated with heregulin, using Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb (upper) or Gab1 Antibody #3232 (lower).

Phospho-Gab1 (Tyr627) Antibody - 3231 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HEK293 cells, untreated or treated with In1B for the indicated times, using Phospho-Gab1 (Tyr627) Antibody (upper) or Gab1 Antibody #3232 (lower).

Gab1 Antibody - 3232 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HEK293, 3T3-L1, C6 and COS cells, using Gab1 Antibody.

Phospho-Gab1 (Tyr659) Antibody - 12745 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of cell extracts from serum-starved T-47D cells, untreated (-) or treated with neuregulin (100 ng/ml, 15 min; +), using Phospho-Gab1 (Tyr659) Antibody (upper) or Gab1 Antibody #3232 (lower).

Gab 1 (C-20) - sc-6292 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Gab 1 (C-20): sc-6292. Western blot analysis of Gab 1 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.

Gab 1 (G-12) - sc-33892 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Gab 1 (G-9) - sc-271848 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gab 1 (G-9): sc-271848. Western blot analysis of Gab 1 expression in non-transfected: sc-117752 (A) and mouse Gab 1 transfected: sc-120377 (B) 293T whole cell lysates.

Gab 1 (H-198) - sc-9049 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Gab 1 (H-198): sc-9049. Western blot analysis of Gab 1 expression in K-562 whole cell lysate.

Gab 1 (H-7) - sc-133191 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gab 1 (H-7): sc-133191. Western blot analysis of Gab 1 expression in non-transfected 293T: sc-117752 (A), human Gab 1 transfected 293T: sc-111467 (B), U-698-M (C) and NIH/3T3 (D) whole cell lysates.

Gab 1 (V-15) - sc-33891 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Gab 1 (V-15): sc-33891. Western blot analysis of Gab 1 expression in non-transfected: sc-117752 (A) and human Gab 1 transfected: sc-111467 (B) 293T whole cell lysates.

p-Gab 1 (Tyr 627) - sc-16794 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-Gab 1 (Tyr 627) - sc-101685 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Gab 1 (Tyr 627): sc-101685. Western blot analysis of phosphorylated Gab 1 expression in untreated (A) and EGF-treated (B) HUVEC whole cell lysates.

Anti-GAB1 (phospho Y627) antibody - ab131458 from Abcam

Host Rabbit
Clonality Polyclonal
Immunofluorescence staining of methanol fixed HeLa cells labelling GAB1 (phospho Y627) with ab131458 antibody at a dilution of 1/100.

Anti-GAB1 (phospho Y627) antibody - ab12863 from Abcam

Host Rabbit
Clonality Polyclonal
Peptide Competition Lysates prepared from NIH3T3 cells left untreated (1) or treated with PDGF (2-5). were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with ab12863 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphosotyrosine containing peptide (4), the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to ab12863 blocks the signal, verifying the specificity of the antibody.

Anti-GAB1 (phospho Y627) antibody - ab47771 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-GAB1 (phospho Y627) antibody (ab47771) at 1/500 dilutionLane 1 : HuvEc cell extract, untreated.Lane 2 : HuvEc cell extract treated with EGF (200ng/ml, 10min)

Anti-GAB1 (phospho Y659) antibody - ab59410 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-GAB1 (phospho Y659) antibody (ab59410) at 1/500 dilutionLane 1 : NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins)Lane 2 : NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins) with immunizing phosphopeptide

Anti-GAB1 antibody - ab59362 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-GAB1 antibody (ab59362) at 1/500 dilutionLane 1 : Extracts from NIH/3T3 cells with no immunizing peptideLane 2 : Extracts from NIH/3T3 cells with immunizing peptideObserved band size : 77 kDa (why is the actual band size different from the predicted?)

Anti-GAB1 antibody - ab47513 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-GAB1 antibody (ab47513)Lane 1 : HUVEC cell extract treated with EGF (200ng/ml, 10 min)Lane 2 : HUVEC cell extract treated with EGF (200ng/ml, 10 min) with peptideLysates/proteins at 30 µg per lane.

Anti-GAB1 antibody - ab117774 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-GAB1 antibody (ab117774) at 0.4 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-GAB1 antibody [1A7] - ab129374 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1A7
ab129374 at 1/200 dilution (green) or negative control (red) staining GAB1 in Jurkat cells by flow cytometry.

Anti-GAB1 antibody [EPR375] - ab133486 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR375
All lanes : Anti-GAB1 antibody [EPR375] (ab133486) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : K562 cell lysateLane 3 : 293T cell lysateLane 4 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-GAB1 antibody [Y423] - ab32093 from Abcam

Host Rabbit
Clonality Monoclonal
Clone Y423
Anti-GAB1 antibody [Y423] (ab32093) at 1/5000 dilution + 293 cell lysate.

Anti-GAB1 antibody produced in rabbit - AV09028 from Sigma-Aldrich

Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: HepG2 (<B>Cat. No. AV09028</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 2.5 μg/mL in cell type HepG2

Anti-phospho-GAB1 (pTyr627) antibody produced in rabbit - SAB4504412 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-phoshpho-GAB1 (pTyr<SUP>627</SUP>): <B>Cat. No. SAB4504412</B>: Immunohistochemistry analysis of paraffin-embedded Human brain gliomas.
Search more