ACTB

Gene Symbol ACTB
Entrez Gene 60
Alt Symbol BRWS1, PS1TP5BP1
Species Human
Gene Type protein-coding
Description actin, beta
Other Description PS1TP5-binding protein 1|actin, cytoplasmic 1|beta cytoskeletal actin
Swissprots Q96HG5 Q96B34 Q75MN2 P60709
Accessions AAA51567 AAP22343 AAS79319 AAX82251 CAI95947 CAR95103 CAS92761 CAY56012 EAW87341 EAW87343 P60709 AK025375 AK058019 AK098751 AK130062 AK130157 AK222925 BAD96645 AK223032 BAD96752 AK223055 BAD96775 AK225414 AK301372 BAG62914 AK304552 BAG65347 AK308277 AK309997 AK316361 BAH14732 BC001301 AAH01301 BC002409 AAH02409 BC004251 AAH04251 BC008633 AAH08633 BC009636 BC012854 AAH12854 BC013380 AAH13380 BC013835 BC014401 BC014861 AAH14861 BC016045 AAH16045 BC023204 BC113036 AAI13037 D28354 BAA05720 DQ407611 ABD66582 DQ471327 ABF01018 DQ890960 ABM81886 DQ894128 ABM85054 EF036500 ABO65086 EF095209 ABL01513 K00790 AAA51578 M28424 AAB16906 V00478 CAA23745 X00351 CAA25099 X63432 CAA45026 XM_006715764 XP_006715827 NM_001101 NP_001092
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Location Cytoplasm, cytoskeleton {ECO:0000269|PubMed:17289661}. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Top Pathways Hippo signaling pathway, Oxytocin signaling pathway, Salmonella infection, Proteoglycans in cancer, Influenza A

PathScan ® Total β-Actin Sandwich ELISA Kit - 7880 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. Treatment of HeLa cells with IFN-α stimulates phosphorylation of Stat3 at Tyr705 as detected by PathScan ® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit #7300, but does not affect the level of β-actin as detected by PathScan ® Total β-Actin Sandwich ELISA Kit #7880. HeLa cells were treated with 100 ng/ml IFN-α for ten minutes at 37ºC before lysis. Absorbance at 450 nm is shown in the top figure, while the corresponding western blots using Phospho-Stat3 (Tyr705) (3E2) Mouse mAb #9138 (right panel) or Total β-Actin (13E5) Rabbit mAb #4970 (left panel) are shown in the bottom figure.

PathScan ® Total β-Actin Sandwich ELISA Antibody Pair - 7881 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
Figure 1. The relationship between the protein concentration of the lysate from HeLa cells and the absorbance at 450 nm using the PathScan ® Total β-Actin Sandwich ELISA Antibody Pair is shown.

SimpleChIP ® Human β-Actin 3' UTR Primers - 13669 from Cell Signaling Technology

Category ChIP Kit
Prices $102.00
Sizes 500 µl (250 PCR reactions)
SimpleChIP ® Human β-Actin 3' UTR Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR ® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R 2 ) were calculated based on the corresponding threshold cycle (C T ) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).

SimpleChIP ® Human β-Actin Promoter Primers - 13653 from Cell Signaling Technology

Category ChIP Kit
Prices $102.00
Sizes 500 µl (250 PCR reactions)
SimpleChIP ® Human β-Actin Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR ® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R 2 ) were calculated based on the corresponding threshold cycle (C T ) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).

Human ACTB / Beta Actin ELISA Kit (Sandwich ELISA) - LS-F10737 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 0.312 ng/ml
Range 0.312-20 ng/ml

Beta-actin, Human, ELISA Kit - CSB-E13298h from ARP American Research Products

Category ELISA Kit
Prices $495.00, $695.00, $696.00, $699.00
Sizes 48 t, 96 t, 5 x 96t, 10 x 96t
Sensitivity 0.078 ng/ml
Range 0.312 ng/ml-20 ng/ml

Actin Beta, ELISA Kit - MBS729157 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS742517 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS736645 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS724618 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS735129 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1 ng/mL.

Actin Beta, ELISA Kit - MBS740507 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS734373 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 1.0 ng/mL.

Actin beta, ELISA Kit - MBS726697 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1 ng/mL.

Actin Beta, ELISA Kit - MBS742842 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS721123 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.1 ng/mL.

Actin Beta, ELISA Kit - MBS736752 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Actin Beta, ELISA Kit - MBS728822 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

actin, beta, ELISA Kit - MBS702655 from MyBioSource

Category ELISA Kit
Prices $250.00, $495.00, $710.00, $2,705.00, $5,145.00
Sizes 24 -strip-wells (Limit 1), 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity <0.078 ng/ml
Range 0.312 ng/ml-20 ng/ml

ELISA Kit for Actin Beta (ACTb) - SEB340Mi from Uscn Life Science Inc.

Category ELISA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.64ng/mL.
Range 1.56-100ng/mL The standard curve concentrations used for the ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL, 1.56ng/mL

CLIA Kit for Actin Beta (ACTb) - SCB340Mi from Uscn Life Science Inc.

Category CLIA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.05ng/mL.
Range 0.14-100ng/mL The standard curve concentrations used for the CLIA’s were 100ng/mL, 33.33ng/mL, 11.11ng/mL, 3.7ng/mL, 1.23ng/mL, 0.41ng/mL, 0.14ng/mL

Human β-actin ELISA Kit - CSB-E13298h from Cusabio

Category ELISA Kit
Sensitivity 0.078 ng/ml
Range 0.312 ng/ml-20 ng/ml

ELISA Kit for Actin Beta (ACTb) - SEB340Mi from Cloud-clone

Category ELISA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.64ng/mL.
Range 1.563-100ng/mL The standard curve concentrations used for the ELISA's were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL

CLIA Kit for Actin Beta (ACTb) - SCB340Mi from Cloud-clone

Category CLIA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.05ng/mL.
Range 0.137-100ng/mL The standard curve concentrations used for the ELISA's were 100ng/mL, 33.33ng/mL, 11.11ng/mL, 3.7ng/mL, 1.235ng/mL, 0.412ng/mL, 0.137ng/mL

Rat ACTB / Beta Actin ELISA Kit (Custom ELISA) - LS-F20160 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity To be determined
Range 0.312-20 ng/ml
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