BRD2

Gene Symbol BRD2
Entrez Gene 6046
Alt Symbol D6S113E, FSH, FSRG1, NAT, RING3, RNF3
Species Human
Gene Type protein-coding
Description bromodomain containing 2
Other Description O27.1.1|bromodomain-containing 2|bromodomain-containing protein 2|female sterile homeotic-related gene 1|really interesting new gene 3 protein
Swissprots B0S7P0 P25440 Q63HQ9 O00699 A2AAU0 Q658Y7 B1AZT1 O00700 Q969U4 Q6P3U2 Q15310 Q5STC9
Accessions AAD24963 AHW47930 AHW47947 AHW47964 AHW47981 CAA65450 CAC69989 EAX03662 EAX03663 EAX03664 EAX03665 EAX03666 P25440 AI983495 AK056504 AK309797 AL832722 CAH56208 AY152720 BC000477 BC001885 BC007715 BC063840 AAH63840 BP271262 BX647233 CAH56179 BX648109 CAH56171 CN398334 D42040 BAA07641 DA127395 DA505721 DC379337 H11588 HY065625 M80613 AAA68890 S78771 X62083 CAA43996 XR_241911 XR_246967 XR_247375 XR_247406 XR_247427 XR_926296 XR_952210 XR_952834 XR_953008 XR_953082 NM_001113182 NP_001106653 NM_001199455 NP_001186384 NM_001199456 NP_001186385 NM_005104 NP_005095 NR_037625 NM_001291986 NP_001278915
Function May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly. {ECO:0000250, ECO:0000269|PubMed:18406326}.
Subcellular Location Nucleus {ECO:0000305}. Note=Detected on chromatin and nucleosomes.

Brd2 (D89B4) Rabbit mAb - 5848 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D89B4
Western blot analysis of extracts from MOLT-4 and NCCIT cells using Brd2 (D89B4) Rabbit mAb.

BRD2 (A-2) - sc-514103 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BRD2 (A-2): sc-514103. Western blot analysis of BRD2 expression in non-transfected: sc-117752 (A) and human BRD2 transfected: sc-117289 (B) 293T whole cell lysates.

BRD2 (D-2) - sc-514102 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BRD2 (D-2): sc-514102. Western blot analysis of BRD2 expression in non-transfected: sc-117752 (A) and human BRD2 transfected: sc-117289 (B) 293T whole cell lysates.

BRD2 (G-4) - sc-393720 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BRD2 (G-4): sc-393720. Western blot analysis of BRD2 expression in non-transfected: sc-117752 (A) and human BRD2 transfected: sc-117289 (B) 293T whole cell lysates.

BRD2 (C-12) - sc-46805 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BRD2 (C-12): sc-46805. Western blot analysis of BRD2 expression in non-transfected: sc-117752 (A) and human BRD2 transfected: sc-117289 (B) 293T whole cell lysates.

BRD2 (K-12) - sc-46806 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BRD2 (K-12): sc-46806. Western blot analysis of BRD2 expression in non-transfected: sc-117752 (A) and human BRD2 transfected: sc-117289 (B) 293T whole cell lysates.

BRD2 (N-16) - sc-46807 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BRD2 (N-16): sc-46807. Western blot analysis of BRD2 expression in non-transfected: sc-117752 (A) and human BRD2 transfected: sc-117289 (B) 293T whole cell lysates.

BRD2 (32-E) - sc-81825 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
BRD2 (32-E): sc-81825. Western blot analysis of BRD2 expression in non-transfected: sc-117752 (A) and human BRD2 transfected: sc-117289 (B) 293T whole cell lysates.

Anti-BRD2 antibody - ab167321 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-BRD2 antibody (ab167321) at 1 µg/mlLane 1 : BRD2-transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.

Anti-BRD2 antibody - ab37633 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-BRD2 antibody (ab37633) at 1/100 dilution + A375 cell lysateSecondaryHRP-anti-rabbit

Anti-BRD2 antibody [EPR7642] - ab139690 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7642
Overlay histogram showing HeLa cells stained with ab139690 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139690, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-BRD2 antibody [EPR7642] (Alexa Fluor® 488) - ab197865 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7642
ab197865 staining BRD2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197865 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-BRD2 antibody [EPR7642] (Alexa Fluor® 647) - ab197866 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7642
ab197866 staining BRD2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197866 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-BRD2 antibody [EPR7642] (HRP) - ab198536 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7642
All lanes : Anti-BRD2 antibody [EPR7642] (HRP) (ab198536) at 1/5000 dilutionLane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : NCCIT (Human pluripotent embryonal teratocarcinoma) Whole Cell LysateLane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-BRD2 / RING3 Antibody - LS-C83014 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BRD2 / RING3 Antibody (clone D89B4) - LS-C183764 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone D89B4

Anti-BRD2 / RING3 Antibody (Internal, AP) - LS-C275658 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BRD2 / RING3 Antibody (Internal, Biotin) - LS-C275661 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BRD2 / RING3 Antibody (Internal, APC) - LS-C275660 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BRD2 / RING3 Antibody (Internal, FITC) - LS-C275662 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BRD2 / RING3 Antibody (Internal, PE) - LS-C275663 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BRD2 / RING3 Antibody (aa675-725) - LS-C286824 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BRD2 / RING3 Antibody (Internal, Azide-free, HRP) - LS-C275664 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BRD2 / RING3 Antibody (C-Terminus) - LS-C31425 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Jurkat Cell Lysate

Anti-BRD2 / RING3 Antibody (aa675-725) - LS-C292272 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
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