IRF3

Gene Symbol IRF3
Entrez Gene 3661
Alt Symbol -
Species Human
Gene Type protein-coding
Description interferon regulatory factor 3
Other Description -
Swissprots Q5FBY1 B2RAZ3 Q5FBY4 Q7Z5G6 Q14653 A8K7L2 Q5FBY2
Accessions AAC68818 AAD05262 EAW52510 EAW52511 EAW52512 EAW52513 Q14653 AA568913 AB102884 BAD89413 AB102886 BAD89415 AB102887 BAD89416 AK057577 AK292027 BAF84716 AK314421 BAG37040 BC000660 AAH00660 BC009395 AAH09395 BC071721 AAH71721 BM477213 BP347627 BU859806 CB961512 DA938709 DA999381 DQ893455 ABM84381 DQ896306 ABM87305 HM357932 ADN86147 HM357933 ADN86148 HM357934 ADN86149 HM357935 ADN86150 HM357936 ADN86151 HM357937 ADN86152 Z56281 CAA91227 XM_006723197 XP_006723260 XM_006723198 XP_006723261 XM_006723199 XP_006723262 XM_006723200 XP_006723263 XM_006723201 XP_006723264 XM_006723202 XP_006723265 XR_935819 XR_935820 NM_001197122 NP_001184051 NM_001197123 NP_001184052 NM_001197124 NP_001184053 NM_001197125 NP_001184054 NM_001197126 NP_001184055 NM_001197127 NP_001184056 NM_001197128 NP_001184057 NM_001571 NP_001562 NR_045568
Function Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG
Subcellular Location Cytoplasm {ECO:0000269|PubMed:10805757}. Nucleus {ECO:0000269|PubMed:10805757}. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
Tissue Specificity Expressed constitutively in a variety of tissues.
Top Pathways Hepatitis C, Pertussis, Toll-like receptor signaling pathway, Measles, Influenza A

Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb - 4947 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 4D4G
Western blot analysis of extracts from HT29 and THP1 cells, control or plpC-transfected (1 hour), using Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb.

IRF-3 (D6I4C) XP ® Rabbit mAb - 11904 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6I4C
Western blot analysis of extracts from various cell lines using IRF-3 (D6I4C) XP ® Rabbit mAb.

IRF-3 (D83B9) Rabbit mAb - 4302 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D83B9
Western blot analysis of extracts from COS-7 cells, mock transfected or transfected with human, mouse or rat IRF-3 constructs, using IRF-3 (D83B9) Rabbit mAb. The human and mouse constructs contain epitope tags.

Phospho-IRF-3 (Ser396) Antibody - 4961 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT29 untreated, or 1 hour post-transfection with poly(rI):poly(rC) using Phospho-IRF-3 (Ser396) Antibody (top) or total IRF-3 Antibody #4962 (bottom).

IRF-3 Antibody - 4962 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947 (upper) and IRF-3 Antibody (lower).

IRF-3 (D-3) - sc-376455 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRF-3 (D-3): sc-376455. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts and Leydig cells.

IRF-3 (FL-425) - sc-9082 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
IRF-3 (FL-425): sc-9082. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

IRF-3 (SL-12) - sc-33641 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRF-3 (SL-12): sc-33641. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

IRF-3 (SL-14) - sc-33642 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRF-3 (SL-14): sc-33642. Western blot analysis of IRF-3 expression in Jurkat (A) and HL-60 (B) whole cell lysates and U-937 nuclear extract (C).

IRF-3 (C-20) - sc-15991 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
IRF-3 (C-20): sc-15991. Western blot analysis of IRF-3 expression in non-transfected: sc-117752 (A) and human IRF-3 transfected: sc-117389 (B) 293T whole cell lysates.

Anti-IRF3 (phospho S385) antibody - ab196035 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IRF3 (phospho S385) antibody (ab196035) at 1/500 dilutionLane 1 : Insulin treated HT29 whole cell lysates with antigen specific peptideLane 2 : Insulin treated HT29 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-IRF3 (phospho S386) antibody - ab192796 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IRF3 (phospho S386) antibody (ab192796) at 1/500 dilutionLane 1 : 293 cell extract with antigen-specific peptideLane 2 : 293 cell extractLane 3 : colo cell extract

Anti-IRF3 (phospho S386) antibody [EPR2346] - ab76493 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2346
ab76493 staining IRF3 (green) in untreated MCF7 cells by Immunocytochemistry/ Immunofluorescense. Nuclei were counterstained blue with DAPI.

Anti-IRF3 (phospho S386) antibody [EPR2346] (Alexa Fluor® 488) - ab193243 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2346
ab193243 staining IRF3 in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab193243 at a working dilution of 1 in 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-IRF3 (phospho S386) antibody [EPR2346] (Alexa Fluor® 647) - ab192981 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2346
ab192981 staining IRF3 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192981 at a working dilution of 1 in 50 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-IRF3 (phospho S396) antibody - ab138449 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-IRF3 (phospho S396) antibody (ab138449) at 1/500 dilution + Jurkat cells treated with 200 ng/ml of EGF for 30 minutes.

Anti-IRF3 antibody - ab25950 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab25950 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab25950 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IRF3 antibody - ab46194 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of IRF3 staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46194, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-IRF3 antibody - ab76367 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IRF3 antibody (ab76367) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-IRF3 antibody - ab25951 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab25951 at 2µg/ml staining IRF3 in Ramos cells by ICC/IF 

Anti-IRF3 antibody - ab74160 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IRF3 antibody (ab74160) at 1/500 dilutionLane 1 : HeLa cell extractsLane 2 : HeLa cell extracts with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-IRF3 antibody - ab59226 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IRF3 antibody (ab59226) at 1/500 dilutionLane 1 : K562 cell extractLane 2 : K562 cell extract with immunizing peptide

Anti-IRF3 antibody - ab84508 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84508, at 1/250 dilution, staining IRF3 in formalin-fixed, paraffin-embedded human breast adenocarcinoma by Immunohistochemistry, using DAB staining.

Anti-IRF3 antibody - ab21680 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-IRF3 antibody (ab21680) at 1/1000 dilutionLane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ugLane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ugSecondaryRabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Anti-IRF3 antibody [3F10] - ab63468 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F10
Immunofluorescence of human HeLa cells stained with ab63468 with Texas-Red and Hoechst 33342 (blue) for nucleus staining.  
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